Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Seeing tobacco mosaic virus through direct electron detectors.
Journal, issue, pages	J Struct Biol, Vol. 189, Issue 2, Page 87-97, Year 2015
Publish date	Dec 17, 2014
Authors	Simon A Fromm / Tanmay A M Bharat / Arjen J Jakobi / Wim J H Hagen / Carsten Sachse /
PubMed Abstract	With the introduction of direct electron detectors (DED) to the field of electron cryo-microscopy, a wave of atomic-resolution structures has become available. As the new detectors still require ...With the introduction of direct electron detectors (DED) to the field of electron cryo-microscopy, a wave of atomic-resolution structures has become available. As the new detectors still require comparative characterization, we have used tobacco mosaic virus (TMV) as a test specimen to study the quality of 3D image reconstructions from data recorded on the two direct electron detector cameras, K2 Summit and Falcon II. Using DED movie frames, we explored related image-processing aspects and compared the performance of micrograph-based and segment-based motion correction approaches. In addition, we investigated the effect of dose deposition on the atomic-resolution structure of TMV and show that radiation damage affects negative carboxyl chains first in a side-chain specific manner. Finally, using 450,000 asymmetric units and limiting the effects of radiation damage, we determined a high-resolution cryo-EM map at 3.35Å resolution. Here, we provide a comparative case study of highly ordered TMV recorded on different direct electron detectors to establish recording and processing conditions that enable structure determination up to 3.2Å in resolution using cryo-EM.
External links	J Struct Biol / PubMed:25528571 / PubMed Central
Methods	EM (helical sym.)
Resolution	3.35 - 4.2 Å
Structure data	EMDB-2833: Cryo-EM structure of TMV reconstructed from micrographs recorded with the K2 Summit DED Method: EM (helical sym.) / Resolution: 4.0 Å EMDB-2834: Cryo-EM structure of TMV reconstructed from micrographs recorded with the Falcon II DED Method: EM (helical sym.) / Resolution: 3.7 Å EMDB-2835: Cryo-EM structure of TMV reconstructed from data ranging from 2.0-6.1 e-/A^2 accumulated dose Method: EM (helical sym.) / Resolution: 3.5 Å EMDB-2836: Cryo-EM structure of TMV reconstructed from data ranging from 6.1-10.2 e-/A^2 accumulated dose Method: EM (helical sym.) / Resolution: 3.5 Å EMDB-2837: Cryo-EM structure of TMV reconstructed from data ranging from 10.1-14.3 e-/A^2 accumulated dose Method: EM (helical sym.) / Resolution: 3.5 Å EMDB-2838: Cryo-EM structure of TMV reconstructed from data ranging from 14.3-18.4 e-/A^2 accumulated dose Method: EM (helical sym.) / Resolution: 3.6 Å EMDB-2839: Cryo-EM structure of TMV reconstructed from data ranging from 18.4-22.5 e-/A^2 accumulated dose Method: EM (helical sym.) / Resolution: 3.8 Å EMDB-2840: Cryo-EM structure of TMV reconstructed from data ranging from 22.5-26.6 e-/A^2 accumulated dose Method: EM (helical sym.) / Resolution: 4.1 Å EMDB-2841: Cryo-EM structure of TMV reconstructed from data ranging from 26.6-30.7 e-/A^2 accumulated dose Method: EM (helical sym.) / Resolution: 4.2 Å 2842 4udv EMDB-2842, PDB-4udv: Cryo-EM structure of TMV at 3.35 A resolution Method: EM (helical sym.) / Resolution: 3.35 Å
Source	tobacco mosaic virus
Keywords	VIRAL PROTEIN / DIRECT ELECTRON DETECTORS / SINGLE PARTICLE HELICAL RECONSTRUCTION / HIGH RESOLUTION