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TitleStructure and transport mechanism of the sodium/proton antiporter MjNhaP1.
Journal, issue, pagesElife, Vol. 3, Page e03583, Year 2014
Publish dateNov 26, 2014
AuthorsCristina Paulino / David Wöhlert / Ekaterina Kapotova / Özkan Yildiz / Werner Kühlbrandt /
PubMed AbstractSodium/proton antiporters are essential for sodium and pH homeostasis and play a major role in human health and disease. We determined the structures of the archaeal sodium/proton antiporter MjNhaP1 ...Sodium/proton antiporters are essential for sodium and pH homeostasis and play a major role in human health and disease. We determined the structures of the archaeal sodium/proton antiporter MjNhaP1 in two complementary states. The inward-open state was obtained by x-ray crystallography in the presence of sodium at pH 8, where the transporter is highly active. The outward-open state was obtained by electron crystallography without sodium at pH 4, where MjNhaP1 is inactive. Comparison of both structures reveals a 7° tilt of the 6 helix bundle. (22)Na(+) uptake measurements indicate non-cooperative transport with an activity maximum at pH 7.5. We conclude that binding of a Na(+) ion from the outside induces helix movements that close the extracellular cavity, open the cytoplasmic funnel, and result in a ∼5 Å vertical relocation of the ion binding site to release the substrate ion into the cytoplasm.
External linksElife / PubMed:25426803 / PubMed Central
MethodsEM (electron crystallography) / X-ray diffraction
Resolution3.5 - 6.0 Å
Structure data

2636

4d0a

EMDB-2636, PDB-4d0a:
3D EM map of the sodium proton antiporter MjNhaP1 from Methanocaldococcus jannaschii
Method: EM (electron crystallography) / Resolution: 6.0 Å

PDB-4czb:
Structure of the sodium proton antiporter MjNhaP1 from Methanocaldococcus jannaschii at pH 8.
Method: X-RAY DIFFRACTION / Resolution: 3.5 Å

Chemicals

ChemComp-K:
Unknown entry

ChemComp-TAM:
TRIS(HYDROXYETHYL)AMINOMETHANE / pH buffer*YM / Tris

Source
  • methanocaldococcus jannaschii (archaea)
  • methanocaldococcus jannaschii dsm 2661 (archaea)
KeywordsMEMBRANE PROTEIN / ANTIPORTER / TRANSPORTER / EXCHANGER / CPA / TRANSPORT PROTEIN

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