Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Molecular architecture of the 40S⋅eIF1⋅eIF3 translation initiation complex.
Journal, issue, pages	Cell, Vol. 158, Issue 5, Page 1123-1135, Year 2014
Publish date	Aug 28, 2014
Authors	Jan P Erzberger / Florian Stengel / Riccardo Pellarin / Suyang Zhang / Tanja Schaefer / Christopher H S Aylett / Peter Cimermančič / Daniel Boehringer / Andrej Sali / Ruedi Aebersold / Nenad Ban /
PubMed Abstract	Eukaryotic translation initiation requires the recruitment of the large, multiprotein eIF3 complex to the 40S ribosomal subunit. We present X-ray structures of all major components of the minimal, ...Eukaryotic translation initiation requires the recruitment of the large, multiprotein eIF3 complex to the 40S ribosomal subunit. We present X-ray structures of all major components of the minimal, six-subunit Saccharomyces cerevisiae eIF3 core. These structures, together with electron microscopy reconstructions, cross-linking coupled to mass spectrometry, and integrative structure modeling, allowed us to position and orient all eIF3 components on the 40S⋅eIF1 complex, revealing an extended, modular arrangement of eIF3 subunits. Yeast eIF3 engages 40S in a clamp-like manner, fully encircling 40S to position key initiation factors on opposite ends of the mRNA channel, providing a platform for the recruitment, assembly, and regulation of the translation initiation machinery. The structures of eIF3 components reported here also have implications for understanding the architecture of the mammalian 43S preinitiation complex and the complex of eIF3, 40S, and the hepatitis C internal ribosomal entry site RNA.
External links	Cell / PubMed:25171412 / PubMed Central
Methods	EM (single particle) / X-ray diffraction
Resolution	2 - 28.1 Å
Structure data	EMDB-2670: Occupied class from the supervised classification of a negatively stained Lachancea kluyveri 40S-eIF1-eIF3 complex. Method: EM (single particle) / Resolution: 28.1 Å EMDB-2671: Unoccupied class from the supervised classification of a negatively stained Lachancea kluyveri 40S-eIF1-eIF3 complex. Method: EM (single particle) / Resolution: 24.6 Å PDB-3j8b: Model of the human eIF3 PCI-MPN octamer docked into the 43S-HCV IRES EM map Method: ELECTRON MICROSCOPY / Resolution: 9.3 Å PDB-3j8c: Model of the human eIF3 PCI-MPN octamer docked into the 43S EM map Method: ELECTRON MICROSCOPY / Resolution: 11.6 Å PDB-4u1c: Crystal structure of the eIF3a/eIF3c PCI-domain heterodimer Method: X-RAY DIFFRACTION / Resolution: 3.5 Å PDB-4u1d: Structure of the PCI domain of translation initiation factor eIF3a Method: X-RAY DIFFRACTION / Resolution: 3.3 Å PDB-4u1e: Crystal structure of the eIF3b-CTD/eIF3i/eIF3g-NTD translation initiation complex Method: X-RAY DIFFRACTION / Resolution: 2.0 Å PDB-4u1f: Crystal structure of middle domain of eukaryotic translation initiation factor eIF3b Method: X-RAY DIFFRACTION / Resolution: 2.2 Å
Chemicals	ChemComp-HOH: WATER / Water
Source	Lachancea kluyveri (fungus) homo sapiens (human) saccharomyces cerevisiae (brewer's yeast)
Keywords	TRANSLATION / translation initiation / eIF3 complex / PCI-domains / PCI domain / beta-propeller