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TitleStructure of the mammalian ribosome-Sec61 complex to 3.4 Å resolution.
Journal, issue, pagesCell, Vol. 157, Issue 7, Page 1632-1643, Year 2014
Publish dateJun 19, 2014
AuthorsRebecca M Voorhees / Israel S Fernández / Sjors H W Scheres / Ramanujan S Hegde /
PubMed AbstractCotranslational protein translocation is a universally conserved process for secretory and membrane protein biosynthesis. Nascent polypeptides emerging from a translating ribosome are either ...Cotranslational protein translocation is a universally conserved process for secretory and membrane protein biosynthesis. Nascent polypeptides emerging from a translating ribosome are either transported across or inserted into the membrane via the ribosome-bound Sec61 channel. Here, we report structures of a mammalian ribosome-Sec61 complex in both idle and translating states, determined to 3.4 and 3.9 Å resolution. The data sets permit building of a near-complete atomic model of the mammalian ribosome, visualization of A/P and P/E hybrid-state tRNAs, and analysis of a nascent polypeptide in the exit tunnel. Unprecedented chemical detail is observed for both the ribosome-Sec61 interaction and the conformational state of Sec61 upon ribosome binding. Comparison of the maps from idle and translating complexes suggests how conformational changes to the Sec61 channel could facilitate translocation of a secreted polypeptide. The high-resolution structure of the mammalian ribosome-Sec61 complex provides a valuable reference for future functional and structural studies.
External linksCell / PubMed:24930395 / PubMed Central
MethodsEM (single particle)
Resolution3.35 - 3.9 Å
Structure data

2644

3j7r

EMDB-2644: Structure of the mammalian ribosome-Sec61 complex
PDB-3j7r: Structure of the translating mammalian ribosome-Sec61 complex
Method: EM (single particle) / Resolution: 3.9 Å

2646

3j7p

EMDB-2646: Structure of the mammalian ribosome-Sec61 complex
PDB-3j7p: Structure of the 80S mammalian ribosome bound to eEF2
Method: EM (single particle) / Resolution: 3.5 Å

2649

3j7o

EMDB-2649: Structure of the mammalian ribosome-Sec61 complex
PDB-3j7o: Structure of the mammalian 60S ribosomal subunit
Method: EM (single particle) / Resolution: 3.35 Å

2650

3j7q

EMDB-2650: Structure of the mammalian ribosome-Sec61 complex
PDB-3j7q: Structure of the idle mammalian ribosome-Sec61 complex
Method: EM (single particle) / Resolution: 3.4 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

Source
  • Sus scrofa domesticus (domestic pig)
  • sus scrofa (pig)
KeywordsRIBOSOME / mammalian / Sec61 / translocation / translation / eEF2

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