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TitleThe full-length cell-cell fusogen EFF-1 is monomeric and upright on the membrane.
Journal, issue, pagesNat Commun, Vol. 5, Page 3912, Year 2014
Publish dateMay 28, 2014
AuthorsTzviya Zeev-Ben-Mordehai / Daven Vasishtan / C Alistair Siebert / Kay Grünewald /
PubMed AbstractFusogens are membrane proteins that remodel lipid bilayers to facilitate membrane merging. Although several fusogen ectodomain structures have been solved, structural information on full-length, ...Fusogens are membrane proteins that remodel lipid bilayers to facilitate membrane merging. Although several fusogen ectodomain structures have been solved, structural information on full-length, natively membrane-anchored fusogens is scarce. Here we present the electron cryo microscopy three-dimensional reconstruction of the Caenorhabditis elegans epithelial fusion failure 1 (EFF-1) protein natively anchored in cell-derived membrane vesicles. This reveals a membrane protruding, asymmetric, elongated monomer. Flexible fitting of a protomer of the EFF-1 crystal structure, which is homologous to viral class-II fusion proteins, shows that EFF-1 has a hairpin monomeric conformation before fusion. These structural insights, when combined with our observations of membrane-merging intermediates between vesicles, enable us to propose a model for EFF-1 mediated fusion. This process, involving identical proteins on both membranes to be fused, follows a mechanism that shares features of SNARE-mediated fusion while using the structural building blocks of the unilaterally acting class-II viral fusion proteins.
External linksNat Commun / PubMed:24867324 / PubMed Central
MethodsEM (subtomogram averaging) / EM (tomography)
Resolution22.2 - 45.0 Å
Structure data

2530

4cyl

EMDB-2530, PDB-4cyl:
Tomographic subvolume average of EFF-1 fusogen on extracellular vesicles
Method: EM (subtomogram averaging) / Resolution: 22.2 Å

EMDB-2531:
Tomographic subvolume average of EFF-1 fusogen on extracellular vesicles
Method: EM (subtomogram averaging)

EMDB-2532:
Tomographic subvolume average of EFF-1 fusogen on extracellular vesicles
Method: EM (subtomogram averaging) / Resolution: 45.0 Å

Source
  • caenorhabditis elegans (invertebrata)
  • Mesocricetus auratus (golden hamster)
KeywordsCELL ADHESION / CELL-CELL FUSION / EXTRACELLULAR FUSION / MEMBRANE FUSION / PRE-FUSION STATE

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