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TitleIdentification of the active sites in the methyltransferases of a transcribing dsRNA virus.
Journal, issue, pagesJ Mol Biol, Vol. 426, Issue 11, Page 2167-2174, Year 2014
Publish dateMay 29, 2014
AuthorsBin Zhu / Chongwen Yang / Hongrong Liu / Lingpeng Cheng / Feng Song / Songjun Zeng / Xiaojun Huang / Gang Ji / Ping Zhu /
PubMed AbstractMany double-stranded RNA (dsRNA) viruses are capable of transcribing and capping RNA within a stable icosahedral viral capsid. The turret of turreted dsRNA viruses belonging to the family Reoviridae ...Many double-stranded RNA (dsRNA) viruses are capable of transcribing and capping RNA within a stable icosahedral viral capsid. The turret of turreted dsRNA viruses belonging to the family Reoviridae is formed by five copies of the turret protein, which contains domains with both 7-N-methyltransferase and 2'-O-methyltransferase activities, and serves to catalyze the methylation reactions during RNA capping. Cypovirus of the family Reoviridae provides a good model system for studying the methylation reactions in dsRNA viruses. Here, we present the structure of a transcribing cypovirus to a resolution of ~3.8Å by cryo-electron microscopy. The binding sites for both S-adenosyl-L-methionine and RNA in the two methyltransferases of the turret were identified. Structural analysis of the turret in complex with RNA revealed a pathway through which the RNA molecule reaches the active sites of the two methyltransferases before it is released into the cytoplasm. The pathway shows that RNA capping reactions occur in the active sites of different turret protein monomers, suggesting that RNA capping requires concerted efforts by at least three turret protein monomers. Thus, the turret structure provides novel insights into the precise mechanisms of RNA methylation.
External linksJ Mol Biol / PubMed:24690366 / PubMed Central
MethodsEM (single particle)
Resolution3.8 Å
Structure data

5926

3j6q

EMDB-5926, PDB-3j6q:
Identification of the active sites in the methyltransferases of a transcribing dsRNA virus
Method: EM (single particle) / Resolution: 3.8 Å

Chemicals

ChemComp-SAH:
S-ADENOSYL-L-HOMOCYSTEINE / S-Adenosyl-L-homocysteine

Source
  • bombyx mori cypovirus 1
KeywordsVIRUS / dsRNA virus / Reoviridae / RNA capping / RNA methyltransferase

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