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TitleMechanosignaling between central apparatus and radial spokes controls axonemal dynein activity.
Journal, issue, pagesJ Cell Biol, Vol. 204, Issue 5, Page 807-819, Year 2014
Publish dateMar 3, 2014
AuthorsToshiyuki Oda / Haruaki Yanagisawa / Toshiki Yagi / Masahide Kikkawa /
PubMed AbstractCilia/flagella are conserved organelles that generate fluid flow in eukaryotes. The bending motion of flagella requires concerted activity of dynein motors. Although it has been reported that the ...Cilia/flagella are conserved organelles that generate fluid flow in eukaryotes. The bending motion of flagella requires concerted activity of dynein motors. Although it has been reported that the central pair apparatus (CP) and radial spokes (RSs) are important for flagellar motility, the molecular mechanism underlying CP- and RS-mediated dynein regulation has not been identified. In this paper, we identified nonspecific intermolecular collision between CP and RS as one of the regulatory mechanisms for flagellar motility. By combining cryoelectron tomography and motility analyses of Chlamydomonas reinhardtii flagella, we show that binding of streptavidin to RS heads paralyzed flagella. Moreover, the motility defect in a CP projection mutant could be rescued by the addition of exogenous protein tags on RS heads. Genetic experiments demonstrated that outer dynein arms are the major downstream effectors of CP- and RS-mediated regulation of flagellar motility. These results suggest that mechanosignaling between CP and RS regulates dynein activity in eukaryotic flagella.
External linksJ Cell Biol / PubMed:24590175 / PubMed Central
MethodsEM (subtomogram averaging)
Resolution68.0 - 72.0 Å
Structure data

EMDB-5845:
Cryo-electron tomography of Chlamydomonas axoneme labeled with streptavidin (Wild type)
Method: EM (subtomogram averaging) / Resolution: 68.0 Å

EMDB-5846:
Cryo-electron tomography of Chlamydomonas axoneme labeled with streptavidin (RSP3 N-terminus)
Method: EM (subtomogram averaging) / Resolution: 69.0 Å

EMDB-5847:
Cryo-electron tomography of Chlamydomonas axoneme labeled with streptavidin (RSP3 C-terminus tag)
Method: EM (subtomogram averaging) / Resolution: 69.0 Å

EMDB-5848:
Cryo-electron tomography of Chlamydomonas axoneme labeled with streptavidin (RSP4 N-terminus)
Method: EM (subtomogram averaging) / Resolution: 69.0 Å

EMDB-5849:
Cryo-electron tomography of Chlamydomonas axoneme labeled with streptavidin (RSP4 C-terminus tag)
Method: EM (subtomogram averaging) / Resolution: 70.0 Å

EMDB-5850:
Cryo-electron tomography of Chlamydomonas axoneme labeled with streptavidin (RSP6 N-terminus tag)
Method: EM (subtomogram averaging) / Resolution: 71.0 Å

EMDB-5851:
Cryo-electron tomography of Chlamydomonas axoneme labeled with streptavidin (RSP6 C-terminus tag)
Method: EM (subtomogram averaging) / Resolution: 70.0 Å

EMDB-5852:
Cryo-electron tomography of Chlamydomonas axoneme labeled with streptavidin (RSP11 C-terminus tag)
Method: EM (subtomogram averaging) / Resolution: 71.0 Å

EMDB-5853:
Cryo-electron tomography of Chlamydomonas axoneme, central pair apparatus
Method: EM (subtomogram averaging) / Resolution: 71.0 Å

EMDB-5854:
Cryo-electron tomography of Chlamydomonas axoneme, central pair apparatus (pf6)
Method: EM (subtomogram averaging) / Resolution: 72.0 Å

EMDB-5855:
Cryo-electron tomography of Chlamydomonas axoneme, central pair apparatus (cpc1)
Method: EM (subtomogram averaging) / Resolution: 71.0 Å

Source
  • Chlamydomonas reinhardtii (plant)

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