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Structure paper

TitleStructural basis for the prion-like MAVS filaments in antiviral innate immunity.
Journal, issue, pagesElife, Vol. 3, Page e01489, Year 2014
Publish dateJan 1, 2014
AuthorsHui Xu / Xiaojing He / Hui Zheng / Lily J Huang / Fajian Hou / Zhiheng Yu / Michael Jason de la Cruz / Brian Borkowski / Xuewu Zhang / Zhijian J Chen / Qiu-Xing Jiang /
PubMed AbstractMitochondrial antiviral signaling (MAVS) protein is required for innate immune responses against RNA viruses. In virus-infected cells MAVS forms prion-like aggregates to activate antiviral signaling ...Mitochondrial antiviral signaling (MAVS) protein is required for innate immune responses against RNA viruses. In virus-infected cells MAVS forms prion-like aggregates to activate antiviral signaling cascades, but the underlying structural mechanism is unknown. Here we report cryo-electron microscopic structures of the helical filaments formed by both the N-terminal caspase activation and recruitment domain (CARD) of MAVS and a truncated MAVS lacking part of the proline-rich region and the C-terminal transmembrane domain. Both structures are left-handed three-stranded helical filaments, revealing specific interfaces between individual CARD subunits that are dictated by electrostatic interactions between neighboring strands and hydrophobic interactions within each strand. Point mutations at multiple locations of these two interfaces impaired filament formation and antiviral signaling. Super-resolution imaging of virus-infected cells revealed rod-shaped MAVS clusters on mitochondria. These results elucidate the structural mechanism of MAVS polymerization, and explain how an α-helical domain uses distinct chemical interactions to form self-perpetuating filaments. DOI: http://dx.doi.org/10.7554/eLife.01489.001.
External linksElife / PubMed:24569476 / PubMed Central
MethodsEM (helical sym.) / X-ray diffraction
Resolution1.944 - 16.4 Å
Structure data

5890

3j6c

EMDB-5890, PDB-3j6c:
Cryo-EM structure of MAVS CARD filament
Method: EM (helical sym.) / Resolution: 9.6 Å

EMDB-5891:
Cryo-EM structure of MAVSdeltaProTM filament
Method: EM (helical sym.) / Resolution: 16.4 Å

PDB-4o9f:
crystal structure of horse MAVS card domain mutant R64C
Method: X-RAY DIFFRACTION / Resolution: 2.348 Å

PDB-4o9l:
crystal structure of horse MAVS card domain mutant E26R
Method: X-RAY DIFFRACTION / Resolution: 1.944 Å

Chemicals

ChemComp-HOH:
WATER / Water

Source
  • homo sapiens (human)
  • equus caballus (horse)
KeywordsSIGNALING PROTEIN / Innate immunity / helical filament / ANTIVIRAL PROTEIN

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