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TitleSelf-assembly of the general membrane-remodeling protein PVAP into sevenfold virus-associated pyramids.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 111, Issue 10, Page 3829-3834, Year 2014
Publish dateMar 11, 2014
AuthorsBertram Daum / Tessa E F Quax / Martin Sachse / Deryck J Mills / Julia Reimann / Özkan Yildiz / Sabine Häder / Cosmin Saveanu / Patrick Forterre / Sonja-Verena Albers / Werner Kühlbrandt / David Prangishvili /
PubMed AbstractViruses have developed a wide range of strategies to escape from the host cells in which they replicate. For egress some archaeal viruses use a pyramidal structure with sevenfold rotational symmetry. ...Viruses have developed a wide range of strategies to escape from the host cells in which they replicate. For egress some archaeal viruses use a pyramidal structure with sevenfold rotational symmetry. Virus-associated pyramids (VAPs) assemble in the host cell membrane from the virus-encoded protein PVAP and open at the end of the infection cycle. We characterize this unusual supramolecular assembly using a combination of genetic, biochemical, and electron microscopic techniques. By whole-cell electron cryotomography, we monitored morphological changes in virus-infected host cells. Subtomogram averaging reveals the VAP structure. By heterologous expression of PVAP in cells from all three domains of life, we demonstrate that the protein integrates indiscriminately into virtually any biological membrane, where it forms sevenfold pyramids. We identify the protein domains essential for VAP formation in PVAP truncation mutants by their ability to remodel the cell membrane. Self-assembly of PVAP into pyramids requires at least two different, in-plane and out-of-plane, protein interactions. Our findings allow us to propose a model describing how PVAP arranges to form sevenfold pyramids and suggest how this small, robust protein may be used as a general membrane-remodeling system.
External linksProc Natl Acad Sci U S A / PubMed:24567401 / PubMed Central
MethodsEM (subtomogram averaging)
Structure data

EMDB-5844:
Subtomogram average of a virus-associated pyramid
Method: EM (subtomogram averaging)

Source
  • Sulfolobus islandicus rod-shaped virus 2

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