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Structure paper

TitleMolecular architecture of a eukaryotic translational initiation complex.
Journal, issue, pagesScience, Vol. 342, Issue 6160, Page 1240585, Year 2013
Publish dateNov 15, 2013
AuthorsIsrael S Fernández / Xiao-Chen Bai / Tanweer Hussain / Ann C Kelley / Jon R Lorsch / V Ramakrishnan / Sjors H W Scheres /
PubMed AbstractThe last step in eukaryotic translational initiation involves the joining of the large and small subunits of the ribosome, with initiator transfer RNA (Met-tRNA(i)(Met)) positioned over the start ...The last step in eukaryotic translational initiation involves the joining of the large and small subunits of the ribosome, with initiator transfer RNA (Met-tRNA(i)(Met)) positioned over the start codon of messenger RNA in the P site. This step is catalyzed by initiation factor eIF5B. We used recent advances in cryo-electron microscopy (cryo-EM) to determine a structure of the eIF5B initiation complex to 6.6 angstrom resolution from <3% of the population, comprising just 5143 particles. The structure reveals conformational changes in eIF5B, initiator tRNA, and the ribosome that provide insights into the role of eIF5B in translational initiation. The relatively high resolution obtained from such a small fraction of a heterogeneous sample suggests a general approach for characterizing the structure of other dynamic or transient biological complexes.
External linksScience / PubMed:24200810 / PubMed Central
MethodsEM (single particle)
Resolution4.3 - 6.6 Å
Structure data

2421

4v8y

EMDB-2421: Molecular architecture of the 80S-eIF5B-Met-itRNAMet Eukaryotic Translation Initiation Complex
PDB-4v8y: Cryo-EM reconstruction of the 80S-eIF5B-Met-itRNAMet Eukaryotic Translation Initiation Complex
Method: EM (single particle) / Resolution: 4.3 Å

2422

4v8z

EMDB-2422: Molecular architecture of the 80S-eIF5B-Met-itRNAMet Eukaryotic Translation Initiation Complex
PDB-4v8z: Cryo-EM reconstruction of the 80S-eIF5B-Met-itRNAMet Eukaryotic Translation Initiation Complex
Method: EM (single particle) / Resolution: 6.6 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-MG:
Unknown entry

ChemComp-OHX:
osmium (III) hexammine

ChemComp-GCP:
PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / GMP-PCP, energy-carrying molecule analogue*YM

Source
  • saccharomyces cerevisiae (brewer's yeast)
  • methanothermobacter thermautotrophicus (archaea)
KeywordsRIBOSOME / RIBOSOME INITIATION COMPLEX / INITIATOR FACTOR EIF5B / SINGLE PARTICLE ANALYSIS

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