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TitleCryoelectron tomography reveals the sequential assembly of bacterial flagella in Borrelia burgdorferi.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 110, Issue 35, Page 14390-14395, Year 2013
Publish dateAug 27, 2013
AuthorsXiaowei Zhao / Kai Zhang / Tristan Boquoi / Bo Hu / M A Motaleb / Kelly A Miller / Milinda E James / Nyles W Charon / Michael D Manson / Steven J Norris / Chunhao Li / Jun Liu /
PubMed AbstractPeriplasmic flagella are essential for the distinctive morphology, motility, and infectious life cycle of the Lyme disease spirochete Borrelia burgdorferi. In this study, we genetically trapped ...Periplasmic flagella are essential for the distinctive morphology, motility, and infectious life cycle of the Lyme disease spirochete Borrelia burgdorferi. In this study, we genetically trapped intermediates in flagellar assembly and determined the 3D structures of the intermediates to 4-nm resolution by cryoelectron tomography. We provide structural evidence that secretion of rod substrates triggers remodeling of the central channel in the flagellar secretion apparatus from a closed to an open conformation. This open channel then serves as both a gateway and a template for flagellar rod assembly. The individual proteins assemble sequentially to form a modular rod. The hook cap initiates hook assembly on completion of the rod, and the filament cap facilitates filament assembly after formation of the mature hook. Cryoelectron tomography and mutational analysis thus combine synergistically to provide a unique structural blueprint of the assembly process of this intricate molecular machine in intact cells.
External linksProc Natl Acad Sci U S A / PubMed:23940315 / PubMed Central
MethodsEM (subtomogram averaging)
Resolution35.0 - 39.0 Å
Structure data

EMDB-5627:
Cryo-electron tomography of in situ flagellar motor of Borrelia burgdorferi
Method: EM (subtomogram averaging) / Resolution: 35.0 Å

EMDB-5628:
Cryo-electron tomography of in situ flagellar motor of Borrelia burgdorferi FliE mutant
Method: EM (subtomogram averaging) / Resolution: 39.0 Å

EMDB-5629:
Cryo-electron tomography of in situ flagellar motor of Borrelia burgdorferi FlgB mutant
Method: EM (subtomogram averaging) / Resolution: 39.0 Å

EMDB-5630:
Cryo-electron tomography of in situ flagellar motor of Borrelia burgdorferi FlgC mutant
Method: EM (subtomogram averaging) / Resolution: 38.0 Å

EMDB-5631:
Cryo-electron tomography of in situ flagellar motor of Borrelia burgdorferi FlhO mutant
Method: EM (subtomogram averaging) / Resolution: 39.0 Å

EMDB-5632:
Cryo-electron tomography of in situ flagellar motor of Borrelia burgdorferi FlgG mutant
Method: EM (subtomogram averaging) / Resolution: 37.0 Å

EMDB-5633:
Cryo-electron tomography of in situ flagellar motor of Borrelia burgdorferi FlgE mutant
Method: EM (subtomogram averaging) / Resolution: 38.0 Å

Source
  • Borrelia burgdorferi (Lyme disease spirochete)

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