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TitleTwo alternative conformations of a voltage-gated sodium channel.
Journal, issue, pagesJ Mol Biol, Vol. 425, Issue 22, Page 4074-4088, Year 2013
Publish dateNov 15, 2013
AuthorsChing-Ju Tsai / Kazutoshi Tani / Katsumasa Irie / Yoko Hiroaki / Takushi Shimomura / Duncan G McMillan / Gregory M Cook / Gebhard F X Schertler / Yoshinori Fujiyoshi / Xiao-Dan Li /
PubMed AbstractActivation and inactivation of voltage-gated sodium channels (Navs) are well studied, yet the molecular mechanisms governing channel gating in the membrane remain unknown. We present two ...Activation and inactivation of voltage-gated sodium channels (Navs) are well studied, yet the molecular mechanisms governing channel gating in the membrane remain unknown. We present two conformations of a Nav from Caldalkalibacillus thermarum reconstituted into lipid bilayers in one crystal at 9Å resolution based on electron crystallography. Despite a voltage sensor arrangement identical with that in the activated form, we observed two distinct pore domain structures: a prominent form with a relatively open inner gate and a closed inner-gate conformation similar to the first prokaryotic Nav structure. Structural differences, together with mutational and electrophysiological analyses, indicated that widening of the inner gate was dependent on interactions among the S4-S5 linker, the N-terminal part of S5 and its adjoining part in S6, and on interhelical repulsion by a negatively charged C-terminal region subsequent to S6. Our findings suggest that these specific interactions result in two conformational structures.
External linksJ Mol Biol / PubMed:23831224
MethodsEM (electron crystallography)
Resolution9.0 Å
Structure data

2347

4bgn

EMDB-2347, PDB-4bgn:
cryo-EM structure of the NavCt voltage-gated sodium channel
Method: EM (electron crystallography) / Resolution: 9.0 Å

Source
  • caldalkalibacillus thermarum (bacteria)
KeywordsTRANSPORT PROTEIN

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