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TitleVisualizing GroEL/ES in the act of encapsulating a folding protein.
Journal, issue, pagesCell, Vol. 153, Issue 6, Page 1354-1365, Year 2013
Publish dateJun 6, 2013
AuthorsDong-Hua Chen / Damian Madan / Jeremy Weaver / Zong Lin / Gunnar F Schröder / Wah Chiu / Hays S Rye /
PubMed AbstractThe GroEL/ES chaperonin system is required for the assisted folding of many proteins. How these substrate proteins are encapsulated within the GroEL-GroES cavity is poorly understood. Using symmetry- ...The GroEL/ES chaperonin system is required for the assisted folding of many proteins. How these substrate proteins are encapsulated within the GroEL-GroES cavity is poorly understood. Using symmetry-free, single-particle cryo-electron microscopy, we have characterized a chemically modified mutant of GroEL (EL43Py) that is trapped at a normally transient stage of substrate protein encapsulation. We show that the symmetric pattern of the GroEL subunits is broken as the GroEL cis-ring apical domains reorient to accommodate the simultaneous binding of GroES and an incompletely folded substrate protein (RuBisCO). The collapsed RuBisCO folding intermediate binds to the lower segment of two apical domains, as well as to the normally unstructured GroEL C-terminal tails. A comparative structural analysis suggests that the allosteric transitions leading to substrate protein release and folding involve concerted shifts of GroES and the GroEL apical domains and C-terminal tails.
External linksCell / PubMed:23746846 / PubMed Central
MethodsEM (single particle)
Resolution8.9 - 15.9 Å
Structure data

2325

3zpz

EMDB-2325: Visualizing GroEL/ES in the Act of Encapsulating a Non-Native Substrate Protein
PDB-3zpz: Visualizing GroEL-ES in the Act of Encapsulating a Non-Native Substrate Protein
Method: EM (single particle) / Resolution: 8.9 Å

2326

3zq0

EMDB-2326: Visualizing GroEL/ES in the Act of Encapsulating a Non-Native Substrate Protein
PDB-3zq0: Visualizing GroEL-ES in the Act of Encapsulating a Non-Native Substrate Protein
Method: EM (single particle) / Resolution: 9.2 Å

2327

3zq1

EMDB-2327: Visualizing GroEL/ES in the Act of Encapsulating a Non-Native Substrate Protein
PDB-3zq1: Visualizing GroEL-ES in the Act of Encapsulating a Non-Native Substrate Protein
Method: EM (single particle) / Resolution: 15.9 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM / Adenosine diphosphate

Source
  • escherichia coli k-12 (bacteria)
  • synthetic construct (others)
  • Rhodospirillum rubrum (bacteria)
  • escherichia coli bl21 (bacteria)
KeywordsCHAPERONE / PROTEIN FOLDING / HETEROGENEITY / ENCAPSULATION / RUBISCO

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