Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structure and assembly of a paramyxovirus matrix protein.
Journal, issue, pages	Proc Natl Acad Sci U S A, Vol. 109, Issue 35, Page 13996-14000, Year 2012
Publish date	Aug 28, 2012
Authors	Anthony J Battisti / Geng Meng / Dennis C Winkler / Lori W McGinnes / Pavel Plevka / Alasdair C Steven / Trudy G Morrison / Michael G Rossmann /
PubMed Abstract	Many pleomorphic, lipid-enveloped viruses encode matrix proteins that direct their assembly and budding, but the mechanism of this process is unclear. We have combined X-ray crystallography and ...Many pleomorphic, lipid-enveloped viruses encode matrix proteins that direct their assembly and budding, but the mechanism of this process is unclear. We have combined X-ray crystallography and cryoelectron tomography to show that the matrix protein of Newcastle disease virus, a paramyxovirus and relative of measles virus, forms dimers that assemble into pseudotetrameric arrays that generate the membrane curvature necessary for virus budding. We show that the glycoproteins are anchored in the gaps between the matrix proteins and that the helical nucleocapsids are associated in register with the matrix arrays. About 90% of virions lack matrix arrays, suggesting that, in agreement with previous biological observations, the matrix protein needs to dissociate from the viral membrane during maturation, as is required for fusion and release of the nucleocapsid into the host's cytoplasm. Structure and sequence conservation imply that other paramyxovirus matrix proteins function similarly.
External links	Proc Natl Acad Sci U S A / PubMed:22891297 / PubMed Central
Methods	EM (subtomogram averaging) / X-ray diffraction
Resolution	2.2 - 45.6 Å
Structure data	EMDB-5448: Cryo-tomography and subtomogram averaging of the Newcastle disease virus matrix protein array Method: EM (subtomogram averaging) / Resolution: 45.6 Å PDB-4g1g: Crystal structure of Newcastle disease virus matrix protein Method: X-RAY DIFFRACTION / Resolution: 2.2 Å PDB-4g1l: Crystal structure of Newcastle disease virus matrix protein Method: X-RAY DIFFRACTION / Resolution: 2.207 Å PDB-4g1o: Crystal structure of Newcastle disease virus matrix protein Method: X-RAY DIFFRACTION / Resolution: 2.2 Å
Chemicals	ChemComp-HOH: WATER / Water
Source	newcastle disease virus
Keywords	VIRAL PROTEIN / Beta strand / Virus assembly / Membrane / Virus / Beta-strand / matrix protein / Viurs