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Structure paper

Title3'-Processing and strand transfer catalysed by retroviral integrase in crystallo.
Journal, issue, pagesEmbo J., Vol. 31, Page 3020-3028, Year 2012
Publish dateMar 17, 2012 (structure data deposition date)
AuthorsHare, S. / Maertens, G.N. / Cherepanov, P.
External linksEmbo J. / PubMed:22580823
MethodsX-ray diffraction
Resolution2.5301 - 3.1501 Å
Structure data

PDB-4e7h:
PFV intasome prior to 3'-processing, Apo form (UI-Apo)
Method: X-RAY DIFFRACTION / Resolution: 2.5701 Å

PDB-4e7i:
PFV intasome freeze-trapped prior to 3'-processing, Mn-bound form (UI-Mn)
Method: X-RAY DIFFRACTION / Resolution: 2.5301 Å

PDB-4e7j:
PFV integrase Target Capture Complex, Apo form (TCC-Apo), at 3.15 A resolution
Method: X-RAY DIFFRACTION / Resolution: 3.1501 Å

PDB-4e7k:
PFV integrase Target Capture Complex (TCC-Mn), freeze-trapped prior to strand transfer, at 3.0 A resolution
Method: X-RAY DIFFRACTION / Resolution: 3.02 Å

PDB-4e7l:
PFV integrase Strand Transfer Complex (STC-Mn*) following reaction in crystallo, at 3.0 A resolution.
Method: X-RAY DIFFRACTION / Resolution: 3.0001 Å

Chemicals

ChemComp-SO4:
SULFATE ION / Sulfate

ChemComp-GOL:
GLYCEROL / Glycerol

ChemComp-HEZ:
HEXANE-1,6-DIOL / 1,6-Hexanediol

ChemComp-ZN:
Unknown entry

ChemComp-HOH:
WATER / Water

ChemComp-MN:
Unknown entry

ChemComp-MES:
2-(N-MORPHOLINO)-ETHANESULFONIC ACID / pH buffer*YM / MES (buffer)

ChemComp-PGE:
TRIETHYLENE GLYCOL / Polyethylene glycol

Source
  • human spumaretrovirus
  • synthetic dna (others)
KeywordsRECOMBINATION/DNA / protein-DNA complex / tetramer / HHCC motif / endonuclease / metal-binding / multifunctional enzyme / nuclease / nucleotidyltransferase / nucleus / transferase / virion / DNA-binding / zinc-binding / viral protein / recombination / viral protein-DNA complex / RECOMBINATION-DNA complex

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