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TitleStructural conservation of the myoviridae phage tail sheath protein fold.
Journal, issue, pagesStructure, Vol. 19, Issue 12, Page 1885-1894, Year 2011
Publish dateDec 7, 2011
AuthorsAnastasia A Aksyuk / Lidia P Kurochkina / Andrei Fokine / Farhad Forouhar / Vadim V Mesyanzhinov / Liang Tong / Michael G Rossmann /
PubMed AbstractBacteriophage phiKZ is a giant phage that infects Pseudomonas aeruginosa, a human pathogen. The phiKZ virion consists of a 1450 Å diameter icosahedral head and a 2000 Å-long contractile tail. The ...Bacteriophage phiKZ is a giant phage that infects Pseudomonas aeruginosa, a human pathogen. The phiKZ virion consists of a 1450 Å diameter icosahedral head and a 2000 Å-long contractile tail. The structure of the whole virus was previously reported, showing that its tail organization in the extended state is similar to the well-studied Myovirus bacteriophage T4 tail. The crystal structure of a tail sheath protein fragment of phiKZ was determined to 2.4 Å resolution. Furthermore, crystal structures of two prophage tail sheath proteins were determined to 1.9 and 3.3 Å resolution. Despite low sequence identity between these proteins, all of these structures have a similar fold. The crystal structure of the phiKZ tail sheath protein has been fitted into cryo-electron-microscopy reconstructions of the extended tail sheath and of a polysheath. The structural rearrangement of the phiKZ tail sheath contraction was found to be similar to that of phage T4.
External linksStructure / PubMed:22153511 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution2.3996 - 19.0 Å
Structure data

EMDB-5331: The cryoEM structure of the bacteriophage phiKZ polysheath
PDB-3j0i: Fitting of the phiKZ gp29PR structure into the cryo-EM density map of the phiKZ polysheath
Method: EM (single particle) / Resolution: 19.0 Å

EMDB-5332: The cryoEM structure of the bacteriophage phiKZ polysheath
PDB-3j0h: Fitting of the bacteriophage phiKZ gp29PR structure into the cryo-EM density map of the phiKZ extended tail sheath
Method: EM (single particle) / Resolution: 18.0 Å

PDB-3spe:
Crystal structure of the tail sheath protein protease resistant fragment from bacteriophage phiKZ
Method: X-RAY DIFFRACTION / Resolution: 2.3996 Å

Chemicals

ChemComp-PO4:
PHOSPHATE ION / Phosphate

ChemComp-GOL:
GLYCEROL / Glycerol

ChemComp-HOH:
WATER / Water

Source
  • pseudomonas phage phikz (virus)
KeywordsSTRUCTURAL PROTEIN / bacteriophage tail sheath

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