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TitleStructure of green-type Rubisco activase from tobacco.
Journal, issue, pagesNat Struct Mol Biol, Vol. 18, Issue 12, Page 1366-1370, Year 2011
Publish dateNov 6, 2011
AuthorsMathias Stotz / Oliver Mueller-Cajar / Susanne Ciniawsky / Petra Wendler / F Ulrich Hartl / Andreas Bracher / Manajit Hayer-Hartl /
PubMed AbstractRubisco, the enzyme that catalyzes the fixation of atmospheric CO(2) in photosynthesis, is subject to inactivation by inhibitory sugar phosphates. Here we report the 2.95-Å crystal structure of ...Rubisco, the enzyme that catalyzes the fixation of atmospheric CO(2) in photosynthesis, is subject to inactivation by inhibitory sugar phosphates. Here we report the 2.95-Å crystal structure of Nicotiana tabacum Rubisco activase (Rca), the enzyme that facilitates the removal of these inhibitors. Rca from tobacco has a classical AAA(+)-protein domain architecture. Although Rca populates a range of oligomeric states when in solution, it forms a helical arrangement with six subunits per turn when in the crystal. However, negative-stain electron microscopy of the active mutant R294V suggests that Rca functions as a hexamer. The residues determining species specificity for Rubisco are located in a helical insertion of the C-terminal domain and probably function in conjunction with the N-domain in Rubisco recognition. Loop segments exposed toward the central pore of the hexamer are required for the ATP-dependent remodeling of Rubisco, resulting in the release of inhibitory sugar.
External linksNat Struct Mol Biol / PubMed:22056769
MethodsEM (single particle) / X-ray diffraction
Resolution2.95 - 20.0 Å
Structure data

1940

3zw6

EMDB-1940: Negative stain EM density of green-type rubisco activase (R294V) from tobacco
PDB-3zw6: MODEL OF HEXAMERIC AAA DOMAIN ARRANGEMENT OF GREEN-TYPE RUBISCO ACTIVASE FROM TOBACCO.
Method: EM (single particle) / Resolution: 20.0 Å

PDB-3t15:
Structure of green-type Rubisco activase from tobacco
Method: X-RAY DIFFRACTION / Resolution: 2.95 Å

Source
  • nicotiana tabacum (common tobacco)
KeywordsPHOTOSYNTHESIS / Rubisco activase / AAA+ protein / NEGATIVE STAIN EM

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