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TitleCryo-EM structure of the ribosome-SecYE complex in the membrane environment.
Journal, issue, pagesNat Struct Mol Biol, Vol. 18, Issue 5, Page 614-621, Year 2011
Publish dateApr 17, 2011
AuthorsJens Frauenfeld / James Gumbart / Eli O van der Sluis / Soledad Funes / Marco Gartmann / Birgitta Beatrix / Thorsten Mielke / Otto Berninghausen / Thomas Becker / Klaus Schulten / Roland Beckmann /
PubMed AbstractThe ubiquitous SecY-Sec61 complex translocates nascent secretory proteins across cellular membranes and integrates membrane proteins into lipid bilayers. Several structures of mostly detergent- ...The ubiquitous SecY-Sec61 complex translocates nascent secretory proteins across cellular membranes and integrates membrane proteins into lipid bilayers. Several structures of mostly detergent-solubilized Sec complexes have been reported. Here we present a single-particle cryo-EM structure of the SecYEG complex in a membrane environment, bound to a translating ribosome, at subnanometer resolution. Using the SecYEG complex reconstituted in a so-called Nanodisc, we could trace the nascent polypeptide chain from the peptidyltransferase center into the membrane. The reconstruction allowed for the identification of ribosome-lipid interactions. The rRNA helix 59 (H59) directly contacts the lipid surface and appears to modulate the membrane in immediate vicinity to the proposed lateral gate of the protein-conducting channel (PCC). On the basis of our map and molecular dynamics simulations, we present a model of a signal anchor-gated PCC in the membrane.
External linksNat Struct Mol Biol / PubMed:21499241 / PubMed Central
MethodsEM (single particle)
Resolution7.1 Å
Structure data

1858

4v6m

EMDB-1858, PDB-4v6m:
Structure of the ribosome-SecYE complex in the membrane environment
Method: EM (single particle) / Resolution: 7.1 Å

Chemicals

ChemComp-PEV:
(1S)-2-{[(2-AMINOETHOXY)(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL STEARATE / POPE, phospholipid*YM / Phosphatidylethanolamine

ChemComp-PGV:
(1R)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL (11E)-OCTADEC-11-ENOATE / phospholipid*YM / Phosphatidylglycerol

Source
  • escherichia coli (E. coli)
  • escherichia coli dh1 (bacteria)
  • escherichia coli o157:h7 (bacteria)
  • escherichia coli k-12 (bacteria)
  • escherichia coli 536 (bacteria)
  • homo sapiens (human)
KeywordsRIBOSOME/RIBOSOMAL PROTEIN / RIBOSOMAL PROTEIN / RIBONUCLEOPROTEIN / NUCLEOTIDE-BINDING / PROTEIN BIOSYNTHESIS / TRANSLATION / ZINC-FINGER / 70S RIBOSOME / RIBOSOME / TRANSLOCON / SECYEG / NANODISC / RIBOSOME-RIBOSOMAL PROTEIN complex

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