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TitleThree-dimensional model of Salmonella's needle complex at subnanometer resolution.
Journal, issue, pagesScience, Vol. 331, Issue 6021, Page 1192-1195, Year 2011
Publish dateMar 4, 2011
AuthorsOliver Schraidt / Thomas C Marlovits /
PubMed AbstractType III secretion systems (T3SSs) are essential virulence factors used by many Gram-negative bacteria to inject proteins that make eukaryotic host cells accessible to invasion. The T3SS core ...Type III secretion systems (T3SSs) are essential virulence factors used by many Gram-negative bacteria to inject proteins that make eukaryotic host cells accessible to invasion. The T3SS core structure, the needle complex (NC), is a ~3.5 megadalton-sized, oligomeric, membrane-embedded complex. Analyzing cryo-electron microscopy images of top views of NCs or NC substructures from Salmonella typhimurium revealed a 24-fold symmetry for the inner rings and a 15-fold symmetry for the outer rings, giving an overall C3 symmetry. Local refinement and averaging showed the organization of the central core and allowed us to reconstruct a subnanometer composite structure of the NC, which together with confident docking of atomic structures reveal insights into its overall organization and structural requirements during assembly.
External linksScience / PubMed:21385715
MethodsEM (single particle)
Resolution6.4 - 11.7 Å
Structure data

EMDB-1871, PDB-2y9k:
Three-dimensional model of Salmonella's needle complex at subnanometer resolution
Method: EM (single particle) / Resolution: 8.3 Å

EMDB-1874: Three-dimensional model of Salmonella's needle complex at subnanometer resolution
PDB-2y9j: THREE-DIMENSIONAL MODEL OF SALMONELLA'S NEEDLE COMPLEX AT SUBNANOMETER RESOLUTION
Method: EM (single particle) / Resolution: 6.4 Å

EMDB-1875:
Three dimensional structure of the injectisome
Method: EM (single particle) / Resolution: 11.7 Å

Chemicals

ChemComp-HOH:
WATER / Water

Source
  • salmonella enterica subsp. enterica serovar typhimurium (bacteria)
KeywordsPROTEIN TRANSPORT / TYPE III SECRETION / IR1 / INNER MEMBRANE RING / C24-FOLD / TYPE III SECRETION SYSTEM / OUTER MEMBRANE RING / SECRETIN FAMILY / C15 FOLD

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