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TitleAtomic model of a cypovirus built from cryo-EM structure provides insight into the mechanism of mRNA capping.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 108, Issue 4, Page 1373-1378, Year 2011
Publish dateJan 25, 2011
AuthorsLingpeng Cheng / Jingchen Sun / Kai Zhang / Zongjun Mou / Xiaoxing Huang / Gang Ji / Fei Sun / Jingqiang Zhang / Ping Zhu /
PubMed AbstractThe cytoplasmic polyhedrosis virus (CPV) from the family Reoviridae belongs to a subgroup of "turreted" reoviruses, in which the mRNA capping activity occurs in a pentameric turret. We report a full ...The cytoplasmic polyhedrosis virus (CPV) from the family Reoviridae belongs to a subgroup of "turreted" reoviruses, in which the mRNA capping activity occurs in a pentameric turret. We report a full atomic model of CPV built from a 3D density map obtained using cryoelectron microscopy. The image data for the 3D reconstruction were acquired exclusively from a CCD camera. Our structure shows that the enzymatic domains of the pentameric turret of CPV are topologically conserved and that there are five unique channels connecting the guanylyltransferase and methyltransferase regions. This structural organization reveals how the channels guide nascent mRNA sequentially to guanylyltransferase, 7-N-methyltransferase, and 2'-O-methyltransferase in the turret, undergoing the highly coordinated mRNA capping activity. Furthermore, by fitting the deduced amino acid sequence of the protein VP5 to 120 large protrusion proteins on the CPV capsid shell, we confirmed that this protrusion protein is encoded by CPV RNA segment 7.
External linksProc Natl Acad Sci U S A / PubMed:21220303 / PubMed Central
MethodsEM (single particle)
Resolution3.9 Å
Structure data

5233

3iz3

EMDB-5233, PDB-3iz3:
CryoEM structure of cytoplasmic polyhedrosis virus
Method: EM (single particle) / Resolution: 3.9 Å

Source
  • bombyx mori cypovirus 1
KeywordsVIRUS / cryoelectron microscopy / cytoplasmic polyhedrosis virus / reovirus / transcription / icosahedral virus

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