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TitleGlycoprotein organization of Chikungunya virus particles revealed by X-ray crystallography.
Journal, issue, pagesNature, Vol. 468, Issue 7324, Page 709-712, Year 2010
Publish dateDec 2, 2010
AuthorsJames E Voss / Marie-Christine Vaney / Stéphane Duquerroy / Clemens Vonrhein / Christine Girard-Blanc / Elodie Crublet / Andrew Thompson / Gérard Bricogne / Félix A Rey /
PubMed AbstractChikungunya virus (CHIKV) is an emerging mosquito-borne alphavirus that has caused widespread outbreaks of debilitating human disease in the past five years. CHIKV invasion of susceptible cells is ...Chikungunya virus (CHIKV) is an emerging mosquito-borne alphavirus that has caused widespread outbreaks of debilitating human disease in the past five years. CHIKV invasion of susceptible cells is mediated by two viral glycoproteins, E1 and E2, which carry the main antigenic determinants and form an icosahedral shell at the virion surface. Glycoprotein E2, derived from furin cleavage of the p62 precursor into E3 and E2, is responsible for receptor binding, and E1 for membrane fusion. In the context of a concerted multidisciplinary effort to understand the biology of CHIKV, here we report the crystal structures of the precursor p62-E1 heterodimer and of the mature E3-E2-E1 glycoprotein complexes. The resulting atomic models allow the synthesis of a wealth of genetic, biochemical, immunological and electron microscopy data accumulated over the years on alphaviruses in general. This combination yields a detailed picture of the functional architecture of the 25 MDa alphavirus surface glycoprotein shell. Together with the accompanying report on the structure of the Sindbis virus E2-E1 heterodimer at acidic pH (ref. 3), this work also provides new insight into the acid-triggered conformational change on the virus particle and its inbuilt inhibition mechanism in the immature complex.
External linksNature / PubMed:21124458
MethodsEM (single particle) / X-ray diffraction
Resolution2.17 - 9 Å
Structure data

PDB-2xfb:
CHIKUNGUNYA E1 E2 ENVELOPE GLYCOPROTEINS FITTED IN SINDBIS VIRUS cryo- EM MAP
Method: ELECTRON MICROSCOPY / Resolution: 9.0 Å

PDB-2xfc:
CHIKUNGUNYA E1 E2 ENVELOPE GLYCOPROTEINS FITTED IN SEMLIKI FOREST VIRUS cryo-EM MAP
Method: ELECTRON MICROSCOPY / Resolution: 9.0 Å

PDB-3n40:
Crystal structure of the immature envelope glycoprotein complex of Chikungunya virus.
Method: X-RAY DIFFRACTION / Resolution: 2.17 Å

PDB-3n41:
Crystal structure of the mature envelope glycoprotein complex (spontaneous cleavage) of Chikungunya virus.
Method: X-RAY DIFFRACTION / Resolution: 3.01 Å

PDB-3n42:
Crystal structures of the mature envelope glycoprotein complex (furin cleavage) of Chikungunya virus.
Method: X-RAY DIFFRACTION / Resolution: 3.0 Å

PDB-3n43:
Crystal structures of the mature envelope glycoprotein complex (trypsin cleavage) of Chikungunya virus.
Method: X-RAY DIFFRACTION / Resolution: 2.58 Å

PDB-3n44:
Crystal structure of the mature envelope glycoprotein complex (trypsin cleavage; Osmium soak) of Chikungunya virus.
Method: X-RAY DIFFRACTION / Resolution: 2.35 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose / N-Acetylglucosamine

ChemComp-ACT:
ACETATE ION / Acetate

ChemComp-HOH:
WATER / Water

ChemComp-OS:
OSMIUM ION / Osmium

Source
  • chikungunya virus
KeywordsVIRUS / ALPHAVIRUS / RECEPTOR BINDING / MEMBRANE FUSION / ICOSAHEDRAL ENVELOPED VIRUS / VIRAL PROTEIN / IMMATURE HETERODIMER

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