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TitleTemplate-free 13-protofilament microtubule-MAP assembly visualized at 8 A resolution.
Journal, issue, pagesJ Cell Biol, Vol. 191, Issue 3, Page 463-470, Year 2010
Publish dateNov 1, 2010
AuthorsFranck J Fourniol / Charles V Sindelar / Béatrice Amigues / Daniel K Clare / Geraint Thomas / Mylène Perderiset / Fiona Francis / Anne Houdusse / Carolyn A Moores /
PubMed AbstractMicrotubule-associated proteins (MAPs) are essential for regulating and organizing cellular microtubules (MTs). However, our mechanistic understanding of MAP function is limited by a lack of detailed ...Microtubule-associated proteins (MAPs) are essential for regulating and organizing cellular microtubules (MTs). However, our mechanistic understanding of MAP function is limited by a lack of detailed structural information. Using cryo-electron microscopy and single particle algorithms, we solved the 8 Å structure of doublecortin (DCX)-stabilized MTs. Because of DCX's unusual ability to specifically nucleate and stabilize 13-protofilament MTs, our reconstruction provides unprecedented insight into the structure of MTs with an in vivo architecture, and in the absence of a stabilizing drug. DCX specifically recognizes the corner of four tubulin dimers, a binding mode ideally suited to stabilizing both lateral and longitudinal lattice contacts. A striking consequence of this is that DCX does not bind the MT seam. DCX binding on the MT surface indirectly stabilizes conserved tubulin-tubulin lateral contacts in the MT lumen, operating independently of the nucleotide bound to tubulin. DCX's exquisite binding selectivity uncovers important insights into regulation of cellular MTs.
External linksJ Cell Biol / PubMed:20974813 / PubMed Central
MethodsEM (single particle) / EM (helical sym.)
Resolution8.2 - 13.5 Å
Structure data

EMDB-1787:
Asymmetric reconstruction of doublecortin-stabilised microtubules decorated with kinesin motor domain
Method: EM (single particle) / Resolution: 13.5 Å

1788

2xrp

EMDB-1788: Doublecortin-stabilised microtubules at secondary structure resolution
PDB-2xrp: Human Doublecortin N-DC Repeat (1MJD) and Mammalian Tubulin (1JFF and 3HKE) Docked into the 8-Angstrom Cryo-EM Map of Doublecortin- Stabilised Microtubules
Method: EM (helical sym.) / Resolution: 8.2 Å

Chemicals

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM / Guanosine diphosphate

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate

Source
  • bos taurus (cattle)
  • homo sapiens (human)
  • Rattus norvegicus (Norway rat)
KeywordsSTRUCTURAL PROTEIN

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