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Structure paper

TitleThe structure of M.EcoKI Type I DNA methyltransferase with a DNA mimic antirestriction protein.
Journal, issue, pagesNucleic Acids Res, Vol. 37, Issue 3, Page 762-770, Year 2009
Publish dateDec 11, 2008
AuthorsChristopher K Kennaway / Agnieszka Obarska-Kosinska / John H White / Irina Tuszynska / Laurie P Cooper / Janusz M Bujnicki / John Trinick / David T F Dryden /
PubMed AbstractType-I DNA restriction-modification (R/M) systems are important agents in limiting the transmission of mobile genetic elements responsible for spreading bacterial resistance to antibiotics. EcoKI, a ...Type-I DNA restriction-modification (R/M) systems are important agents in limiting the transmission of mobile genetic elements responsible for spreading bacterial resistance to antibiotics. EcoKI, a Type I R/M enzyme from Escherichia coli, acts by methylation- and sequence-specific recognition, leading to either methylation of DNA or translocation and cutting at a random site, often hundreds of base pairs away. Consisting of one specificity subunit, two modification subunits, and two DNA translocase/endonuclease subunits, EcoKI is inhibited by the T7 phage antirestriction protein ocr, a DNA mimic. We present a 3D density map generated by negative-stain electron microscopy and single particle analysis of the central core of the restriction complex, the M.EcoKI M(2)S(1) methyltransferase, bound to ocr. We also present complete atomic models of M.EcoKI in complex with ocr and its cognate DNA giving a clear picture of the overall clamp-like operation of the enzyme. The model is consistent with a large body of experimental data on EcoKI published over 40 years.
External linksNucleic Acids Res / PubMed:19074193 / PubMed Central
MethodsEM (single particle)
Resolution18.0 Å
Structure data

1534

2y7c

2y7h

EMDB-1534: EcoKI type I RM methyltransferase with DNA mimic Ocr. Negative stain 3D.
PDB-2y7c: Atomic model of the Ocr-bound methylase complex from the Type I restriction-modification enzyme EcoKI (M2S1). Based on fitting into EM map 1534.
PDB-2y7h: Atomic model of the DNA-bound methylase complex from the Type I restriction-modification enzyme EcoKI (M2S1). Based on fitting into EM map 1534.
Method: EM (single particle) / Resolution: 18.0 Å

Chemicals

ChemComp-SAM:
S-ADENOSYLMETHIONINE / S-Adenosyl methionine

Source
  • escherichia coli (E. coli)
  • enterobacteria phage t7 (virus)
KeywordsTRANSFERASE / TRANSFERASE/DNA / TRANSFERASE-DNA COMPLEX

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