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TitleProgression of the ribosome recycling factor through the ribosome dissociates the two ribosomal subunits.
Journal, issue, pagesMol Cell, Vol. 27, Issue 2, Page 250-261, Year 2007
Publish dateJul 20, 2007
AuthorsChandana Barat / Partha P Datta / V Samuel Raj / Manjuli R Sharma / Hideko Kaji / Akira Kaji / Rajendra K Agrawal /
PubMed AbstractAfter the termination step of translation, the posttermination complex (PoTC), composed of the ribosome, mRNA, and a deacylated tRNA, is processed by the concerted action of the ribosome-recycling ...After the termination step of translation, the posttermination complex (PoTC), composed of the ribosome, mRNA, and a deacylated tRNA, is processed by the concerted action of the ribosome-recycling factor (RRF), elongation factor G (EF-G), and GTP to prepare the ribosome for a fresh round of protein synthesis. However, the sequential steps of dissociation of the ribosomal subunits, and release of mRNA and deacylated tRNA from the PoTC, are unclear. Using three-dimensional cryo-electron microscopy, in conjunction with undecagold-labeled RRF, we show that RRF is capable of spontaneously moving from its initial binding site on the 70S Escherichia coli ribosome to a site exclusively on the large 50S ribosomal subunit. This movement leads to disruption of crucial intersubunit bridges and thereby to the dissociation of the two ribosomal subunits, the central event in ribosome recycling. Results of this study allow us to propose a model of ribosome recycling.
External linksMol Cell / PubMed:17643374
MethodsEM (single particle)
Resolution15.3 - 18.4 Å
Structure data

EMDB-1369:
Progression of the ribosome recycling factor through the ribosome dissociates the two ribosomal subunits.
Method: EM (single particle) / Resolution: 18.4 Å

EMDB-1370:
Progression of the ribosome recycling factor through the ribosome dissociates the two ribosomal subunits.
Method: EM (single particle) / Resolution: 15.3 Å

Source
  • Escherichia coli (E. coli)

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