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TitleRF3 induces ribosomal conformational changes responsible for dissociation of class I release factors.
Journal, issue, pagesCell, Vol. 129, Issue 5, Page 929-941, Year 2007
Publish dateJun 1, 2007
AuthorsHaixiao Gao / Zhihong Zhou / Urmila Rawat / Chenhui Huang / Lamine Bouakaz / Chernhoe Wang / Zhihong Cheng / Yuying Liu / Andrey Zavialov / Richard Gursky / Suparna Sanyal / Måns Ehrenberg / Joachim Frank / Haiwei Song /
PubMed AbstractDuring translation termination, class II release factor RF3 binds to the ribosome to promote rapid dissociation of a class I release factor (RF) in a GTP-dependent manner. We present the crystal ...During translation termination, class II release factor RF3 binds to the ribosome to promote rapid dissociation of a class I release factor (RF) in a GTP-dependent manner. We present the crystal structure of E. coli RF3*GDP, which has a three-domain architecture strikingly similar to the structure of EF-Tu*GTP. Biochemical data on RF3 mutants show that a surface region involving domains II and III is important for distinct steps in the action cycle of RF3. Furthermore, we present a cryo-electron microscopy (cryo-EM) structure of the posttermination ribosome bound with RF3 in the GTP form. Our data show that RF3*GTP binding induces large conformational changes in the ribosome, which break the interactions of the class I RF with both the decoding center and the GTPase-associated center of the ribosome, apparently leading to the release of the class I RF.
External linksCell / PubMed:17540173
MethodsEM (single particle) / X-ray diffraction
Resolution2.8 - 15.5 Å
Structure data

1302

2o0f

EMDB-1302: RF3 induces ribosomal conformational changes responsible for dissociation of class I release factors.
PDB-2o0f: Docking of the modified RF3 X-ray structure into cryo-EM map of E.coli 70S ribosome bound with RF3
Method: EM (single particle) / Resolution: 15.5 Å

PDB-2h5e:
Crystal structure of E.coli polypeptide release factor RF3
Method: X-RAY DIFFRACTION / Resolution: 2.8 Å

Chemicals

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM / Guanosine diphosphate

ChemComp-HOH:
WATER / Water

Source
  • escherichia coli (E. coli)
KeywordsTRANSLATION / BETA BARREL / RF3 / Ribosome / cryo-EM

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