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TitleElongation arrest by SecM via a cascade of ribosomal RNA rearrangements.
Journal, issue, pagesMol Cell, Vol. 22, Issue 4, Page 533-543, Year 2006
Publish dateMay 19, 2006
AuthorsKakoli Mitra / Christiane Schaffitzel / Felcy Fabiola / Michael S Chapman / Nenad Ban / Joachim Frank /
PubMed AbstractIn E. coli, the SecM nascent polypeptide causes elongation arrest, while interacting with 23S RNA bases A2058 and A749-753 in the exit tunnel of the large ribosomal subunit. We compared atomic models ...In E. coli, the SecM nascent polypeptide causes elongation arrest, while interacting with 23S RNA bases A2058 and A749-753 in the exit tunnel of the large ribosomal subunit. We compared atomic models fitted by real-space refinement into cryo-electron microscopy reconstructions of a pretranslocational and SecM-stalled E. coli ribosome complex. A cascade of RNA rearrangements propagates from the exit tunnel throughout the large subunit, affecting intersubunit bridges and tRNA positions, which in turn reorient small subunit RNA elements. Elongation arrest could result from the inhibition of mRNA.(tRNAs) translocation, E site tRNA egress, and perhaps translation factor activation at the GTPase-associated center. Our study suggests that the specific secondary and tertiary arrangement of ribosomal RNA provides the basis for internal signal transduction within the ribosome. Thus, the ribosome may itself have the ability to regulate its progression through translation by modulating its structure and consequently its receptivity to activation by cofactors.
External linksMol Cell / PubMed:16713583
MethodsEM (single particle)
Resolution15 Å
Structure data

PDB-4v4v:
Structure of a pre-translocational E. coli ribosome obtained by fitting atomic models for RNA and protein components into cryo-EM map EMD-1056
Method: ELECTRON MICROSCOPY / Resolution: 15.0 Å

PDB-4v4w:
Structure of a SecM-stalled E. coli ribosome complex obtained by fitting atomic models for RNA and protein components into cryo-EM map EMD-1143
Method: ELECTRON MICROSCOPY / Resolution: 15.0 Å

Source
  • escherichia coli (E. coli)
KeywordsRIBOSOME / SecM; nascent chain; signal transduction; RNA world; polypeptide exit tunnel; translocation; ribosome; elongation arrest / SecM; nascent chain; signal transduction; RNA world; polypeptide exit tunnel; translocation; ribosome; elongation arrest; protein-conducting channel

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