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TitleStructure of the E. coli protein-conducting channel bound to a translating ribosome.
Journal, issue, pagesNature, Vol. 438, Issue 7066, Page 318-324, Year 2005
Publish dateNov 17, 2005
AuthorsKakoli Mitra / Christiane Schaffitzel / Tanvir Shaikh / Florence Tama / Simon Jenni / Charles L Brooks / Nenad Ban / Joachim Frank /
PubMed AbstractSecreted and membrane proteins are translocated across or into cell membranes through a protein-conducting channel (PCC). Here we present a cryo-electron microscopy reconstruction of the Escherichia ...Secreted and membrane proteins are translocated across or into cell membranes through a protein-conducting channel (PCC). Here we present a cryo-electron microscopy reconstruction of the Escherichia coli PCC, SecYEG, complexed with the ribosome and a nascent chain containing a signal anchor. This reconstruction shows a messenger RNA, three transfer RNAs, the nascent chain, and detailed features of both a translocating PCC and a second, non-translocating PCC bound to mRNA hairpins. The translocating PCC forms connections with ribosomal RNA hairpins on two sides and ribosomal proteins at the back, leaving a frontal opening. Normal mode-based flexible fitting of the archaeal SecYEbeta structure into the PCC electron microscopy densities favours a front-to-front arrangement of two SecYEG complexes in the PCC, and supports channel formation by the opening of two linked SecY halves during polypeptide translocation. On the basis of our observation in the translocating PCC of two segregated pores with different degrees of access to bulk lipid, we propose a model for co-translational protein translocation.
External linksNature / PubMed:16292303 / PubMed Central
MethodsEM (single particle)
Resolution14.9 Å
Structure data

1143

2akh

2aki

EMDB-1143: Structure of the E. coli protein-conducting channel bound to a translating ribosome.
PDB-2akh: Normal mode-based flexible fitted coordinates of a non-translocating SecYEG protein-conducting channel into the cryo-EM map of a SecYEG-nascent chain-70S ribosome complex from E. coli
PDB-2aki: Normal mode-based flexible fitted coordinates of a translocating SecYEG protein-conducting channel into the cryo-EM map of a SecYEG-nascent chain-70S ribosome complex from E. coli
Method: EM (single particle) / Resolution: 14.9 Å

Source
  • escherichia coli (E. coli)
  • unidentified (others)
KeywordsPROTEIN TRANSPORT / Translocation / Transmembrane / Transport

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