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TitleMechanism for the disassembly of the posttermination complex inferred from cryo-EM studies.
Journal, issue, pagesMol Cell, Vol. 18, Issue 6, Page 663-674, Year 2005
Publish dateJun 10, 2005
AuthorsNing Gao / Andrey V Zavialov / Wen Li / Jayati Sengupta / Mikel Valle / Richard P Gursky / Måns Ehrenberg / Joachim Frank /
PubMed AbstractRibosome recycling, the disassembly of the posttermination complex after each round of protein synthesis, is an essential step in mRNA translation, but its mechanism has remained obscure. In ...Ribosome recycling, the disassembly of the posttermination complex after each round of protein synthesis, is an essential step in mRNA translation, but its mechanism has remained obscure. In eubacteria, recycling is catalyzed by RRF (ribosome recycling factor) and EF-G (elongation factor G). By using cryo-electron microscopy, we have obtained two density maps, one of the RRF bound posttermination complex and one of the 50S subunit bound with both EF-G and RRF. Comparing the two maps, we found domain I of RRF to be in the same orientation, while domain II in the EF-G-containing 50S subunit is extensively rotated (approximately 60 degrees) compared to its orientation in the 70S complex. Mapping the 50S conformation of RRF onto the 70S posttermination complex suggests that it can disrupt the intersubunit bridges B2a and B3, and thus effect a separation of the two subunits. These observations provide the structural basis for the mechanism by which the posttermination complex is split into subunits by the joint action of RRF and EF-G.
External linksMol Cell / PubMed:15949441
MethodsEM (single particle)
Resolution14.1 - 15.5 Å
Structure data

1127

1zn0

1zn1

EMDB-1127: Mechanism for the disassembly of the posttermination complex inferred from cryo-EM studies.
PDB-1zn0: Coordinates of RRF and EF-G fitted into Cryo-EM map of the 50S subunit bound with both EF-G (GDPNP) and RRF
PDB-1zn1: Coordinates of RRF fitted into Cryo-EM map of the 70S post-termination complex
Method: EM (single particle) / Resolution: 15.5 Å

1128

1zn0

1zn1

EMDB-1128: Mechanism for the disassembly of the posttermination complex inferred from cryo-EM studies.
PDB-1zn0: Coordinates of RRF and EF-G fitted into Cryo-EM map of the 50S subunit bound with both EF-G (GDPNP) and RRF
PDB-1zn1: Coordinates of RRF fitted into Cryo-EM map of the 70S post-termination complex
Method: EM (single particle) / Resolution: 14.1 Å

Source
  • escherichia coli (E. coli)
KeywordsTRANSLATION/BIOSYNTHETIC PROTEIN/RNA / ribosome recycling factor / elongation factor G / 50S subunit / TRANSLATION-BIOSYNTHETIC PROTEIN-RNA COMPLEX / BIOSYNTHETIC/structural protein/RNA / 70S / post-termination complex / BIOSYNTHETIC-structural protein-RNA COMPLEX

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