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TitleStructural basis of pore formation by the bacterial toxin pneumolysin.
Journal, issue, pagesCell, Vol. 121, Issue 2, Page 247-256, Year 2005
Publish dateApr 22, 2005
AuthorsSarah J Tilley / Elena V Orlova / Robert J C Gilbert / Peter W Andrew / Helen R Saibil /
PubMed AbstractThe bacterial toxin pneumolysin is released as a soluble monomer that kills target cells by assembling into large oligomeric rings and forming pores in cholesterol-containing membranes. Using cryo-EM ...The bacterial toxin pneumolysin is released as a soluble monomer that kills target cells by assembling into large oligomeric rings and forming pores in cholesterol-containing membranes. Using cryo-EM and image processing, we have determined the structures of membrane-surface bound (prepore) and inserted-pore oligomer forms, providing a direct observation of the conformational transition into the pore form of a cholesterol-dependent cytolysin. In the pore structure, the domains of the monomer separate and double over into an arch, forming a wall sealing the bilayer around the pore. This transformation is accomplished by substantial refolding of two of the four protein domains along with deformation of the membrane. Extension of protein density into the bilayer supports earlier predictions that the protein inserts beta hairpins into the membrane. With an oligomer size of up to 44 subunits in the pore, this assembly creates a transmembrane channel 260 A in diameter lined by 176 beta strands.
External linksCell / PubMed:15851031
MethodsEM (single particle)
Resolution28.0 - 29.0 Å
Structure data

1106

2bk2

EMDB-1106: Structural basis of pore formation by the bacterial toxin pneumolysin.
PDB-2bk2: The prepore structure of pneumolysin, obtained by fitting the alpha carbon trace of perfringolysin O into a cryo-EM map
Method: EM (single particle) / Resolution: 28.0 Å

1107

2bk1

EMDB-1107: Structural basis of pore formation by the bacterial toxin pneumolysin.
PDB-2bk1: The pore structure of pneumolysin, obtained by fitting the alpha carbon trace of perfringolysin O into a cryo-EM map
Method: EM (single particle) / Resolution: 29.0 Å

1108

2bk1

EMDB-1108: Structural basis of pore formation by the bacterial toxin pneumolysin.
PDB-2bk1: The pore structure of pneumolysin, obtained by fitting the alpha carbon trace of perfringolysin O into a cryo-EM map
Method: EM (single particle) / Resolution: 28.0 Å

Source
  • synthetic construct (others)
  • Streptococcus pneumoniae (bacteria)
  • clostridium perfringens (bacteria)
KeywordsTOXIN / CYTOLYSIS / HEMOLYSIS / THIOL-ACTIVATED CYTOLYSIN / CRYOEM / CYTOLYTIC PROTEIN

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