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TitleDomain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation.
Journal, issue, pagesEMBO J, Vol. 23, Issue 5, Page 1008-1019, Year 2004
Publish dateMar 10, 2004
AuthorsChristian M T Spahn / Maria G Gomez-Lorenzo / Robert A Grassucci / Rene Jørgensen / Gregers R Andersen / Roland Beckmann / Pawel A Penczek / Juan P G Ballesta / Joachim Frank /
PubMed AbstractAn 11.7-A-resolution cryo-EM map of the yeast 80S.eEF2 complex in the presence of the antibiotic sordarin was interpreted in molecular terms, revealing large conformational changes within eEF2 and ...An 11.7-A-resolution cryo-EM map of the yeast 80S.eEF2 complex in the presence of the antibiotic sordarin was interpreted in molecular terms, revealing large conformational changes within eEF2 and the 80S ribosome, including a rearrangement of the functionally important ribosomal intersubunit bridges. Sordarin positions domain III of eEF2 so that it can interact with the sarcin-ricin loop of 25S rRNA and protein rpS23 (S12p). This particular conformation explains the inhibitory action of sordarin and suggests that eEF2 is stalled on the 80S ribosome in a conformation that has similarities with the GTPase activation state. A ratchet-like subunit rearrangement (RSR) occurs in the 80S.eEF2.sordarin complex that, in contrast to Escherichia coli 70S ribosomes, is also present in vacant 80S ribosomes. A model is suggested, according to which the RSR is part of a mechanism for moving the tRNAs during the translocation reaction.
External linksEMBO J / PubMed:14976550 / PubMed Central
MethodsEM (single particle)
Resolution11.7 Å
Structure data

1067

4v4b

EMDB-1067: Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation.
PDB-4v4b: Structure of the ribosomal 80S-eEF2-sordarin complex from yeast obtained by docking atomic models for RNA and protein components into a 11.7 A cryo-EM map.
Method: EM (single particle) / Resolution: 11.7 Å

Source
  • saccharomyces cerevisiae (brewer's yeast)
KeywordsRIBOSOME / 80S ribosome / 40S ribosomal subunit / eEF2 / tRNA translocation / sordarin / cryo-EM

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