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Structure paper

TitleModular Assembly of the Bacterial Large Ribosomal Subunit.
Journal, issue, pagesCell, Vol. 167, Issue 6, Page 1610-1622.e15, Year 2016
Publish dateDec 1, 2016
AuthorsJoseph H Davis / Yong Zi Tan / Bridget Carragher / Clinton S Potter / Dmitry Lyumkis / James R Williamson /
PubMed AbstractThe ribosome is a complex macromolecular machine and serves as an ideal system for understanding biological macromolecular assembly. Direct observation of ribosome assembly in vivo is difficult, as ...The ribosome is a complex macromolecular machine and serves as an ideal system for understanding biological macromolecular assembly. Direct observation of ribosome assembly in vivo is difficult, as few intermediates have been isolated and thoroughly characterized. Herein, we deploy a genetic system to starve cells of an essential ribosomal protein, which results in the accumulation of assembly intermediates that are competent for maturation. Quantitative mass spectrometry and single-particle cryo-electron microscopy reveal 13 distinct intermediates, which were each resolved to ∼4-5 Å resolution and could be placed in an assembly pathway. We find that ribosome biogenesis is a parallel process, that blocks of structured rRNA and proteins assemble cooperatively, and that the entire process is dynamic and can be "re-routed" through different pathways as needed. This work reveals the complex landscape of ribosome assembly in vivo and provides the requisite tools to characterize additional assembly pathways for ribosomes and other macromolecular machines.
External linksCell / PubMed:27912064 / PubMed Central
MethodsEM (single particle)
Resolution3.7 - 7.9 Å
Structure data

EMDB-8434:
Structure of bL17-depleted large ribosomal subunit assembly intermediate - Class A
Method: EM (single particle) / Resolution: 6.5 Å

EMDB-8440:
Structure of bL17-depleted large ribosomal subunit assembly intermediate - Class B
Method: EM (single particle) / Resolution: 4.5 Å

EMDB-8441:
Structure of bL17-depleted large ribosomal subunit assembly intermediate - Class C
Method: EM (single particle) / Resolution: 3.7 Å

EMDB-8442:
Structure of bL17-depleted large ribosomal subunit assembly intermediate - Class C1
Method: EM (single particle) / Resolution: 4.5 Å

EMDB-8443:
Structure of bL17-depleted large ribosomal subunit assembly intermediate - Class C2
Method: EM (single particle) / Resolution: 4.6 Å

EMDB-8444:
Structure of bL17-depleted large ribosomal subunit assembly intermediate - Class C3
Method: EM (single particle) / Resolution: 4.0 Å

EMDB-8445:
Structure of bL17-depleted large ribosomal subunit assembly intermediate - Class D
Method: EM (single particle) / Resolution: 4.0 Å

EMDB-8446:
Structure of bL17-depleted large ribosomal subunit assembly intermediate - Class D1
Method: EM (single particle) / Resolution: 4.7 Å

EMDB-8447:
Structure of bL17-depleted large ribosomal subunit assembly intermediate - Class D2
Method: EM (single particle) / Resolution: 4.9 Å

EMDB-8448:
Structure of bL17-depleted large ribosomal subunit assembly intermediate - Class D3
Method: EM (single particle) / Resolution: 4.6 Å

EMDB-8449:
Structure of bL17-depleted large ribosomal subunit assembly intermediate - Class D4
Method: EM (single particle) / Resolution: 4.7 Å

EMDB-8450:
Structure of bL17-depleted large ribosomal subunit assembly intermediate - Class E
Method: EM (single particle) / Resolution: 3.7 Å

EMDB-8451:
Structure of bL17-depleted large ribosomal subunit assembly intermediate - Class E1
Method: EM (single particle) / Resolution: 4.2 Å

EMDB-8452:
Structure of bL17-depleted large ribosomal subunit assembly intermediate - Class E2
Method: EM (single particle) / Resolution: 4.5 Å

EMDB-8453:
Structure of bL17-depleted large ribosomal subunit assembly intermediate - Class E3
Method: EM (single particle) / Resolution: 5.0 Å

EMDB-8455:
Structure of bL17-depleted large ribosomal subunit assembly intermediate - Class E4
Method: EM (single particle) / Resolution: 4.5 Å

EMDB-8456:
Structure of bL17-depleted large ribosomal subunit assembly intermediate - Class E5
Method: EM (single particle) / Resolution: 4.9 Å

EMDB-8457:
Structure of 30S Ribosome - Class F
Method: EM (single particle) / Resolution: 7.9 Å

Source
  • Escherichia coli (E. coli)

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