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Structure paper

TitleStructural basis for the gating mechanism of the type 2 ryanodine receptor RyR2.
Journal, issue, pagesScience, Vol. 354, Issue 6310, Year 2016
Publish dateOct 21, 2016
AuthorsWei Peng / Huaizong Shen / Jianping Wu / Wenting Guo / Xiaojing Pan / Ruiwu Wang / S R Wayne Chen / Nieng Yan /
PubMed AbstractRyR2 is a high-conductance intracellular calcium (Ca) channel that controls the release of Ca from the sarco(endo)plasmic reticulum of a variety of cells. Here, we report the structures of RyR2 from ...RyR2 is a high-conductance intracellular calcium (Ca) channel that controls the release of Ca from the sarco(endo)plasmic reticulum of a variety of cells. Here, we report the structures of RyR2 from porcine heart in both the open and closed states at near-atomic resolutions determined using single-particle electron cryomicroscopy. Structural comparison reveals a breathing motion of the overall cytoplasmic region resulted from the interdomain movements of amino-terminal domains (NTDs), Helical domains, and Handle domains, whereas almost no intradomain shifts are observed in these armadillo repeats-containing domains. Outward rotations of the Central domains, which integrate the conformational changes of the cytoplasmic region, lead to the dilation of the cytoplasmic gate through coupled motions. Our structural and mutational characterizations provide important insights into the gating and disease mechanism of RyRs.
External linksScience / PubMed:27708056
MethodsEM (single particle)
Resolution4.2 - 4.4 Å
Structure data

EMDB-9528, PDB-5go9:
Cryo-EM structure of RyR2 in closed state
Method: EM (single particle) / Resolution: 4.4 Å

EMDB-9529, PDB-5goa:
Cryo-EM structure of RyR2 in open state
Method: EM (single particle) / Resolution: 4.2 Å

Chemicals

ChemComp-ZN:
Unknown entry

Source
  • sus scrofa (pig)
KeywordsTRANSPORT PROTEIN / Membrane protein / Channel / Membrane protein; Channel

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