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TitleThe architecture of respiratory supercomplexes.
Journal, issue, pagesNature, Vol. 537, Issue 7622, Page 644-648, Year 2016
Publish dateSep 29, 2016
AuthorsJames A Letts / Karol Fiedorczuk / Leonid A Sazanov /
PubMed AbstractMitochondrial electron transport chain complexes are organized into supercomplexes responsible for carrying out cellular respiration. Here we present three architectures of mammalian (ovine) ...Mitochondrial electron transport chain complexes are organized into supercomplexes responsible for carrying out cellular respiration. Here we present three architectures of mammalian (ovine) supercomplexes determined by cryo-electron microscopy. We identify two distinct arrangements of supercomplex CICIIICIV (the respirasome)-a major 'tight' form and a minor 'loose' form (resolved at the resolution of 5.8 Å and 6.7 Å, respectively), which may represent different stages in supercomplex assembly or disassembly. We have also determined an architecture of supercomplex CICIII at 7.8 Å resolution. All observed density can be attributed to the known 80 subunits of the individual complexes, including 132 transmembrane helices. The individual complexes form tight interactions that vary between the architectures, with complex IV subunit COX7a switching contact from complex III to complex I. The arrangement of active sites within the supercomplex may help control reactive oxygen species production. To our knowledge, these are the first complete architectures of the dominant, physiologically relevant state of the electron transport chain.
External linksNature / PubMed:27654913
MethodsEM (single particle)
Resolution5.8 - 7.8 Å
Structure data

8128

5j7y

EMDB-8128: Architecture of ovine respiratory supercomplex (I-III2-IV) conformation loose
PDB-5j7y: Architecture of loose respirasome
Method: EM (single particle) / Resolution: 6.7 Å

8129

5j8k

EMDB-8129: Architecture of ovine respiratory supercomplex (I-III2)
PDB-5j8k: Architecture of supercomplex I-III2
Method: EM (single particle) / Resolution: 6.7 Å

8130

5j4z

EMDB-8130: Architecture of ovine respiratory supercomplex (I-III2-IV) conformation tight
PDB-5j4z: Architecture of tight respirasome
Method: EM (single particle) / Resolution: 5.8 Å

Chemicals

ChemComp-SF4:
IRON/SULFUR CLUSTER

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE

ChemComp-CU:
COPPER (II) ION

ChemComp-HEA:
HEME-A

ChemComp-CUA:
DINUCLEAR COPPER ION

Source
  • ovis aries (sheep)
  • Sheep (sheep)
KeywordsELECTRON TRANSPORT / membrane protein complex / supercomplex / respirasome / MEMBRANE PROTEIN / Mitochondrial supercomplex / NADH-ubiquinone oxidoreductase / coenzyme Q-cytochrome c Oxidoreductase / cytochrome c oxidase

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