3D Electron Microscopy (3D-EM) Data Navigator [English / 日本語]
Top Gallery List Diagram Statistics Viewer Document
PDBj>EM Navigator>Document page
Glossary

3D electron microscopy (3D-EM)

A generic term of electron microscopic analyses to obtain 3D structurres

  • Analyses such as electron tomography, single particle analysis, and electron diffraction are included.
    Aggregation statesMethods generally used
    "individual structure"electron tomography
    "single particle"single particle analysis
    "icosahedral"single particle analysis
    "helical"helical reconstruction / single particle analysis
    "2D-crystal"electron diffraction / Fourier filtering
  • Characteristics compared to X-ray crystallography and NMR:
  • Advantage: Wider applicability of sample (not require high-purity or high-concentration smaple, and useful for more huge, complex, flexible, and less uniform sample)
  • Disadvantage: Lower resolution (hard to get atomic-level resolution data)
  • electron cryo microscopy (cryo-EM)

    Electron microscopy where the sample is studied at cryogenic temperatures.

    Cryo-electron microscopy - Wikipedia

    EMDB: Electron Microscopy Data Bank

    Data bank for 3D-EM

  • EMDataBank.org
  • "Movies out of date"

    Movies with this annotation may not be up-to-date.

    EMDB map data are sometimes remediated. Unfortunately, the process of making movies in the EM Navigaotr are not fully automated, and it is very hard to remake the all to catch up. So, some movies and movie parameters are based on the older data, and may be improper for new map data.