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- EMDB-8361: Structure and assembly model for the Trypanosoma cruzi 60S riboso... -

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Basic information

Entry
Database: EMDB / ID: EMD-8361
TitleStructure and assembly model for the Trypanosoma cruzi 60S ribosomal subunit
Map dataStructure and assembly model for the Trypanosoma cruzi 60S ribosomal subunit
Sample
  • Complex: trypanosoma cruzi 60S ribosomal subunit
    • RNA: x 8 types
    • Protein or peptide: x 38 types
  • Ligand: x 3 types
Function / homology
Function and homology information


maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / nucleosome assembly / nucleosome / large ribosomal subunit / 5S rRNA binding / cytosolic large ribosomal subunit / membrane => GO:0016020 / rRNA binding / ribosome / structural constituent of ribosome ...maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / nucleosome assembly / nucleosome / large ribosomal subunit / 5S rRNA binding / cytosolic large ribosomal subunit / membrane => GO:0016020 / rRNA binding / ribosome / structural constituent of ribosome / translation / DNA binding / RNA binding / metal ion binding / cytosol / cytoplasm
Similarity search - Function
Linker histone H1/H5 / Ribosomal protein L30e / Ribosomal protein L28e / Ribosomal L28e/Mak16 / Ribosomal L28e protein family / Ribosomal protein L29e / Ribosomal L29e protein family / Eukaryotic Ribosomal Protein L27, KOW domain / Ribosomal protein L44e / Ribosomal protein L38e ...Linker histone H1/H5 / Ribosomal protein L30e / Ribosomal protein L28e / Ribosomal L28e/Mak16 / Ribosomal L28e protein family / Ribosomal protein L29e / Ribosomal L29e protein family / Eukaryotic Ribosomal Protein L27, KOW domain / Ribosomal protein L44e / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal protein L27e / Ribosomal protein L27e superfamily / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L38e protein family / Ribosomal L22e protein family / 60S ribosomal protein L35 / Ribosomal protein L30e, conserved site / Ribosomal protein L44 / Ribosomal protein L34Ae / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / Ribosomal L27e protein family / Ribosomal Protein L6, KOW domain / Ribosomal protein L30/YlxQ / Ribosomal protein L13e / Ribosomal protein L13e / Ribosomal protein L34e / Ribosomal protein L37ae / 60S ribosomal protein L6E / Ribosomal protein L19, eukaryotic / Ribosomal protein L35A / Ribosomal protein L36e / Ribosomal protein L36e domain superfamily / Ribosomal protein L36e / Ribosomal protein L35A superfamily / Ribosomal protein L44e signature. / Ribosomal protein L7A/L8 / 60S ribosomal protein L4, C-terminal domain / 60S ribosomal protein L18a/ L20, eukaryotes / Ribosomal protein L7, eukaryotic / Ribosomal protein L6e / Ribosomal protein L30, N-terminal / Ribosomal L30 N-terminal domain / Ribosomal L37ae protein family / Ribosomal protein L35Ae / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal protein L39e, conserved site / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / 60S ribosomal protein L4 C-terminal domain / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / Ribosomal protein L31e / Ribosomal protein L31e domain superfamily / Ribosomal_L31e / Ribosomal protein L30e signature 1. / Ribosomal protein L21e / Ribosomal protein L21e, conserved site / Ribosomal protein L21 superfamily / Ribosomal protein L14e domain / Ribosomal protein 60S L18 and 50S L18e / 60S ribosomal protein L19 / Ribosomal protein L4/L1e, eukaryotic/archaeal, conserved site / Ribosomal protein L37e, conserved site / Ribosomal protein L37e / Ribosomal protein L14 / Ribosomal protein L39e signature. / Ribosomal protein L21e / Ribosomal_L15e / Ribosomal protein L15e / Ribosomal protein L15e core domain superfamily / Ribosomal protein L14 / Ribosomal protein L31e / Ribosomal protein L37ae/L37e / Ribosomal protein L14, KOW motif / Ribosomal protein L24e-related / Ribosomal protein L22/L17, eukaryotic/archaeal / Ribosomal protein L24e/L24 superfamily / Ribosomal protein L39e / Ribosomal protein L26/L24, eukaryotic/archaeal / Ribosomal protein L39e domain superfamily / Ribosomal_L19e / Ribosomal protein L13, eukaryotic/archaeal / Ribosomal protein L19/L19e / Ribosomal protein L19/L19e, domain 1 / Ribosomal protein L19/L19e superfamily / Ribosomal protein L4, eukaryotic and archaeal type / Ribosomal protein L19e / Ribosomal protein L18e / Ribosomal L39 protein / Ribosomal protein L37e / Ribosomal protein L24e / Ribosomal L15 / Ribosomal protein L7, eukaryotic/archaeal / Ribosomal protein L7/L30 / Ribosomal proteins L26 eukaryotic, L24P archaeal / Ribosomal protein L5 eukaryotic/L18 archaeal / Ribosomal large subunit proteins 60S L5, and 50S L18
Similarity search - Domain/homology
60S ribosomal protein L28 / 60S ribosomal protein L11, putative / 60S ribosomal protein L29 / 60S ribosomal protein L4, putative / Ribosomal protein L15 / 60S ribosomal protein L5, putative / 60S ribosomal protein L44, putative / 60S ribosomal protein L32, putative / 60S ribosomal protein L37a, putative / 60S ribosomal protein L34, putative ...60S ribosomal protein L28 / 60S ribosomal protein L11, putative / 60S ribosomal protein L29 / 60S ribosomal protein L4, putative / Ribosomal protein L15 / 60S ribosomal protein L5, putative / 60S ribosomal protein L44, putative / 60S ribosomal protein L32, putative / 60S ribosomal protein L37a, putative / 60S ribosomal protein L34, putative / 60S ribosomal protein L17, putative / Ribosomal protein L21E (60S), putative / 60S ribosomal protein L26, putative / 60S ribosomal protein L30, putative / 60S ribosomal protein L6, putative / 60S ribosomal protein L7, putative / 60S ribosomal protein L2, putative / 40S ribosomal protein L14, putative / Ribosomal protein L38, putative / Ribosomal protein L39, putative / Ribosomal protein L37 / 60S ribosomal protein L18a / 60S ribosomal protein L22, putative / 60S ribosomal protein L18 / Ribosomal protein L24, putative / 60S ribosomal protein L35, putative / 60S ribosomal protein L23a, putative / 60S ribosomal protein L27A/L29, putative / 60S ribosomal protein L23, putative / Ribosomal protein L35A, putative / 60S ribosomal protein L13 / 60S ribosomal protein L7a / Ribosomal protein L19-like protein / 60S ribosomal protein L27 / Ribosomal protein L13 / Ribosomal protein L36 / 60S ribosomal subunit protein L31 / 60S ribosomal protein L13a
Similarity search - Component
Biological speciesTrypanosoma cruzi (eukaryote)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.54 Å
AuthorsLiu Z / Gutierrez-Vargas C / Wei J / Grassucci RA / Ramesh M / Espina N / Sun M / Tutuncuoglu B / Madison-Antenucci S / Woolford Jr JL ...Liu Z / Gutierrez-Vargas C / Wei J / Grassucci RA / Ramesh M / Espina N / Sun M / Tutuncuoglu B / Madison-Antenucci S / Woolford Jr JL / Tong L / Frank J
Funding support United States, 5 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM29169 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM028301 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)35GM118093 United States
Howard Hughes Medical Institute (HHMI)HHMI United States
National Science Foundation (NSF, United States)GRFP DGE-11-44155 United States
CitationJournal: Proc Natl Acad Sci U S A / Year: 2016
Title: Structure and assembly model for the Trypanosoma cruzi 60S ribosomal subunit.
Authors: Zheng Liu / Cristina Gutierrez-Vargas / Jia Wei / Robert A Grassucci / Madhumitha Ramesh / Noel Espina / Ming Sun / Beril Tutuncuoglu / Susan Madison-Antenucci / John L Woolford / Liang Tong / Joachim Frank /
Abstract: Ribosomes of trypanosomatids, a family of protozoan parasites causing debilitating human diseases, possess multiply fragmented rRNAs that together are analogous to 28S rRNA, unusually large rRNA ...Ribosomes of trypanosomatids, a family of protozoan parasites causing debilitating human diseases, possess multiply fragmented rRNAs that together are analogous to 28S rRNA, unusually large rRNA expansion segments, and r-protein variations compared with other eukaryotic ribosomes. To investigate the architecture of the trypanosomatid ribosomes, we determined the 2.5-Å structure of the Trypanosoma cruzi ribosome large subunit by single-particle cryo-EM. Examination of this structure and comparative analysis of the yeast ribosomal assembly pathway allowed us to develop a stepwise assembly model for the eight pieces of the large subunit rRNAs and a number of ancillary "glue" proteins. This model can be applied to the characterization of Trypanosoma brucei and Leishmania spp. ribosomes as well. Together with other details, our atomic-level structure may provide a foundation for structure-based design of antitrypanosome drugs.
History
DepositionSep 30, 2016-
Header (metadata) releaseOct 12, 2016-
Map releaseOct 12, 2016-
UpdateDec 18, 2019-
Current statusDec 18, 2019Processing site: RCSB / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.09
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by height
  • Surface level: 0.09
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-5t5h
  • Surface level: 0.09
  • Imaged by UCSF Chimera
  • Download
Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_8361.png

Downloads & links

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Map

FileDownload / File: emd_8361.map.gz / Format: CCP4 / Size: 46.4 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationStructure and assembly model for the Trypanosoma cruzi 60S ribosomal subunit
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.05 Å/pix.
x 230 pix.
= 240.35 Å		1.05 Å/pix.
x 230 pix.
= 240.35 Å		1.05 Å/pix.
x 230 pix.
= 240.35 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.045 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.07 / Movie #1: 0.09
Minimum - Maximum-0.45471793 - 0.8159538
Average (Standard dev.)0.0041980413 (±0.053391784)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions230230230
Spacing230230230
CellA=B=C: 240.34999 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.0451.0451.045
M x/y/z230230230
origin x/y/z0.0000.0000.000
length x/y/z240.350240.350240.350
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS230230230
D min/max/mean-0.4550.8160.004

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Supplemental data

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Sample components

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Entire : trypanosoma cruzi 60S ribosomal subunit

EntireName: trypanosoma cruzi 60S ribosomal subunit
Components
  • Complex: trypanosoma cruzi 60S ribosomal subunit
    • RNA: RNA LARGE SUBUNIT ALPHA
    • RNA: RNA LARGE SUBUNIT BETA
    • RNA: 5.8S rRNA5.8S ribosomal RNA
    • RNA: 5S rRNA5S ribosomal RNA
    • RNA: srRNA1
    • RNA: srRNA3
    • RNA: srRNA2
    • RNA: srRNA4
    • Protein or peptide: 60S ribosomal protein L18
    • Protein or peptide: 60S ribosomal protein L11
    • Protein or peptide: 60S ribosomal protein L13
    • Protein or peptide: 60S ribosomal protein L13a
    • Protein or peptide: 40S ribosomal protein L14
    • Protein or peptide: Ribosomal protein L15
    • Protein or peptide: 60S ribosomal protein L17
    • Protein or peptide: 60S ribosomal protein L18a
    • Protein or peptide: Ribosomal protein L19-like proteinRibosome
    • Protein or peptide: Ribosomal protein L21E (60S)Ribosome
    • Protein or peptide: 60S ribosomal protein L22
    • Protein or peptide: 60S ribosomal protein L23
    • Protein or peptide: 60S ribosomal protein L23a
    • Protein or peptide: Ribosomal protein L24
    • Protein or peptide: 60S ribosomal protein L26
    • Protein or peptide: Ribosomal protein L27
    • Protein or peptide: 60S ribosomal protein L27A/L29Ribosome
    • Protein or peptide: 60S ribosomal protein L28
    • Protein or peptide: Ribosomal protein L29
    • Protein or peptide: 60S ribosomal protein L2
    • Protein or peptide: Ribosomal protein L1360S ribosomal protein L13
    • Protein or peptide: 60S ribosomal protein L30
    • Protein or peptide: 60S ribosomal subunit protein L31
    • Protein or peptide: 60S ribosomal protein L32
    • Protein or peptide: 60S ribosomal protein L34
    • Protein or peptide: 60S ribosomal protein L35
    • Protein or peptide: Ribosomal protein L35ARibosome
    • Protein or peptide: Ribosomal protein L36
    • Protein or peptide: Ribosomal protein L37
    • Protein or peptide: 60S ribosomal protein L37a
    • Protein or peptide: Ribosomal protein L38
    • Protein or peptide: Ribosomal protein L39
    • Protein or peptide: 60S ribosomal protein L4
    • Protein or peptide: 60S ribosomal protein L44
    • Protein or peptide: 60S ribosomal protein L5
    • Protein or peptide: 60S ribosomal protein L6
    • Protein or peptide: 60S ribosomal protein L7
    • Protein or peptide: Ribosomal protein L7a-like proteinRibosome
  • Ligand: MAGNESIUM ION
  • Ligand: ZINC ION
  • Ligand: water

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Supramolecule #1: trypanosoma cruzi 60S ribosomal subunit

SupramoleculeName: trypanosoma cruzi 60S ribosomal subunit / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#46
Source (natural)Organism: Trypanosoma cruzi (eukaryote)

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Macromolecule #1: RNA LARGE SUBUNIT ALPHA

MacromoleculeName: RNA LARGE SUBUNIT ALPHA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 414.400031 KDa
SequenceString: CAGACCUGAG UGUGGCAGGA CUACCCGCCG AACUUAAGCA UAUUACUAAG CGGAGGAAAA GAAAACAACC GUGAUUCUCU CAGUCAGCG GCGAGCGAAG AGGGAUCCAA CUCGUUGCCG AAUCGGUUCU UAUAGGAACU UGAGUUGUGG CAAUGGUGCA G GAAUGGUG ...String:
CAGACCUGAG UGUGGCAGGA CUACCCGCCG AACUUAAGCA UAUUACUAAG CGGAGGAAAA GAAAACAACC GUGAUUCUCU CAGUCAGCG GCGAGCGAAG AGGGAUCCAA CUCGUUGCCG AAUCGGUUCU UAUAGGAACU UGAGUUGUGG CAAUGGUGCA G GAAUGGUG AAAUUCAAAG CAAACCCGUU GCUACAUCAU GUGAGUAUUG AGCCAAAGAA GGUGUUAGCC CAUUGAGCCA UG AACCUAA GAGAGUAGCA CUGCUUGGGA GUGCAGUGUC AAGCUGG(OMC)AG GUAUGUGUCU GCCAAAGUUA AAUACAGAG UAGGAAGACC GAUAGCGAAC AAGUAGCGUG AGCGAAAGUU UGAAAAGC(A2M)C UUUGGAAAGA GAGUGACAUA GACCUG AAG UCGUGACAAU AAUGGAAGUA CCUCCAUUUC GCCAAAACCA GCCUCUACUG AGCAAGAACG AGGUCAUGGU UUUGGCG GA AAUGGGGUGC CCCCACCCGU CUUGA(A2M)A(OMC)(A2M)C GGACCAAGGA GU(OMC)A(A2M)ACAGA CGCGCAAG G GAGAAGAUAU CAGUACUUCU CUCGUACUGA AAGGGAGAUU GUGGUGUGCA CACUUGC(OMC)CA ACACC(OMG)ACC (7MG)GCCCCGGGU UGAGUUGGAG UGU(OMG)CCUG(OMU)U UGGACCC(OMG)A(A2M) AGGUGGUGAA CUAUGCCUGA ACAAGGUGA AGCCCGGGGA AACUCGGGUG GAGGCCUGUA GCGAUGCUG(A2M) C(7MG)UGCAAAU(OMC) GCUCGCAUG AUUUGGGU(A2M)U (5MC)G(OMG)AGCGAAA GACUCAUCGA ACCACCUAGU AGCUGGUUCA CAUCGAAGUU UCCC (OMU)CAGG AUAGCUGGUG CUUGAAAAAG UAUUGUGCGG UAAAGCUUUA AA(OMU)GUGCAAC UUGCCAAAGC AAGGUAU UA AGGCACCAAG UGGGCCUUCU UCGGUAAGC(OMG) GAGCAGGCGA UGCGGAAUGA ACCGCUUAGG GAUAUGUGGC GUCA AAACU UAUGGGCUCA CGAACAAUGG A(OMU)GACGCUCA AGCCCAAUUA GUGCC(5MC)AUUA UUCCUUUGGU AAGGGCG AG AAAGUGUAGC CCUUCCGAUG UAGAUGUGGC GUGGAGGUAA GGACGAAGCU GAUGGCGUGA GCCUCAGAUG GACCAGCC U CUAGUGCA(OMG)A U(OMC)UUGGUUGG CGU(A2M)(OMG)CAAAG AUCUAACGGA AC(OMG)UUGGAUA CUGGAGC (7MG)G GGAAGGAUUU CGUGCCAACG GCACUCGUAC ACGAGUUGUU CGGAUACUGA GCACAACGUU ACACCGUUUU UAGG A(A2M)AAG GGGCAACAGA GAACCUGGGA UUAUAUUCCA AAAAGAAAUU GCAUG

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Macromolecule #2: RNA LARGE SUBUNIT BETA

MacromoleculeName: RNA LARGE SUBUNIT BETA / type: rna / ID: 2 / Number of copies: 1
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 303.89975 KDa
SequenceString: ACUGCAGACC GUACU(OMC)AUCA CCGCAUCAGG UCCCCAAGCA UACA(A2M)UGCUC UGGUACAUAG AGAAA(OMG)G (OMU)A CA(OMC)UCAGGGA AGUCGGCAAA AUAGAUGCGU AAGUUCGCAA GAAGCAUUGG CUCUGAGGGC AACAACUCAG AACUGCUUA ...String:
ACUGCAGACC GUACU(OMC)AUCA CCGCAUCAGG UCCCCAAGCA UACA(A2M)UGCUC UGGUACAUAG AGAAA(OMG)G (OMU)A CA(OMC)UCAGGGA AGUCGGCAAA AUAGAUGCGU AAGUUCGCAA GAAGCAUUGG CUCUGAGGGC AACAACUCAG AACUGCUUA CGGCGGGGAA UCCAACUGU(A2M) UAAUUAAAAC AUAGGUUUGU GAUGCAUCCA AGUGGUGUAU GUCGCAA AC UGAUUUCUGC C(OMC)AGUGCUCU GU(OMG)AAGAGGU UCACCGGCGC GGGUAAACGG CAAGGUAGC(5MC) AA (A2M)UGCCUC (OMG)UCUUCCAAU UAGAGACGCG CAU(7MG)AAUGGA UUAAUGAGAU UCCCUCUGU(OMC) CCGAGUU (A2M)C UAUCUAGCGA AACGACAGUC AAGGGAACGG ACUUG(A2M)AGGG CUAAGCGGGG AAAGAAGACC CU(OMG)UUG AGU UUGAC(OMU)CCAG UCUGGCUCUG UGAGGCGAGC UUCACUUAUC GAAUGAAGAG ACCAAUGGGU UUCGCGUAGU CUG CGGGCU AUGCACCGUC UCU(7MG)AACCAA CGGA(7MG)UCUUG UUUCGAGACA UCUGCCAGAU GGGGA(OMG)UUUG GC UGGGGCG GCAUAUCUGU UACACGACAA CGCAGGU(OMG)UC CUAAGGCGAG CUCAGUGGGA UCUCACGUAG AACACAAGC GUAAAAGCUU GCUUGAUUAA CGAUUUCCAG UACGAAUCGA GACUGCGAAA GCAAGGCCAG CGAUCCUUUG CGUA(OMU)G AAC AAAUCAUACC A(OMG)A(OMG)GUGUCA GAAUAAUUAC (OMC)ACAG(OMG)GAUA ACUGGCUUAU CCUUCGAUGU C GG(OMC)UCUUC CUAACCUAGC GGCUAAGGGU UGGAU(OMU)(OMG)UUC ACCCACUGAC AGGGAACGUG (A2M)GCUGG GUU UAGA(OMC)CGUCG UGAGACAGGU UGGUUUUACC CUACUUAGCU GGAAAUUGCG AGAAAAAAAA AAUUUUUUAA AAU UUUUUU UUUUUUUACU UGGUACGAAA AAAA

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Macromolecule #3: 5.8S rRNA

MacromoleculeName: 5.8S rRNA / type: rna / ID: 3 / Number of copies: 1
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 54.425363 KDa
SequenceString: AACGUG(OMU)CGC GAUGGAUGAC UUGGCUUCCU AUUUCGUUGA A(7MG)(A2M)ACGCAGC AAAGUGCGAU AAGUGGUA U CAAUU(OMG)CAGA AUCAUUCAAU UACCGAAUCU UUGAA(OMC)GCAA ACGGCGCA(OMU)G GGAGAAGCUC CCCGGAG UC AUCCCCGUGC ...String:
AACGUG(OMU)CGC GAUGGAUGAC UUGGCUUCCU AUUUCGUUGA A(7MG)(A2M)ACGCAGC AAAGUGCGAU AAGUGGUA U CAAUU(OMG)CAGA AUCAUUCAAU UACCGAAUCU UUGAA(OMC)GCAA ACGGCGCA(OMU)G GGAGAAGCUC CCCGGAG UC AUCCCCGUGC AUGCCAUAUU UCUC(A2M)GU(OMG)UC G

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Macromolecule #4: 5S rRNA

MacromoleculeName: 5S rRNA / type: rna / ID: 4 / Number of copies: 1
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 38.047582 KDa
SequenceString:
GGGUACGACC AUACUUGGCC GAGUGCACCA CAUCCCGUCC GAUUUGUGAA GUUAAGCGGC CACAGGCCUC GUUAGUACGG CGAUCAGUG AUGGCGCUGG AACCCGGGGU GCUGCAAUC

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Macromolecule #5: srRNA1

MacromoleculeName: srRNA1 / type: rna / ID: 5 / Number of copies: 1
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 46.982832 KDa
SequenceString:
GUGGAAAUGC GGCCAGGUGA CCAAUCAAUC CUCCCACGGU GAGCUUUCUU UUCACCAUAA UCCACUUGGG CCUUUCGAGU UGUUCGGAA CGGGGGCUCA AGAUUGAAAA AUGCAGUUGU GAGUUCUAUU AAAGCAAAAA CCUGGGG

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Macromolecule #6: srRNA3

MacromoleculeName: srRNA3 / type: rna / ID: 6 / Number of copies: 1
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 14.572682 KDa
SequenceString:
CGAAUCGCCA CUUCUCUCGG CGCCAAGUAC CUUCAUAUUC ACAAGA

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Macromolecule #7: srRNA2

MacromoleculeName: srRNA2 / type: rna / ID: 7 / Number of copies: 1
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 39.608434 KDa
SequenceString:
GGUAUCGUGA GGGUAGUACG AGGUGUACUC CCAUGCCGU(OMG) CCUCUGGUUU CUGGAGUUUG UCGAACGGCA AGUGCU CCG ACGCUAUCGC ACGGUGGUUA GCCAGUCCCA AGACCAGAUG CCCA

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Macromolecule #8: srRNA4

MacromoleculeName: srRNA4 / type: rna / ID: 8 / Number of copies: 1
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 29.374498 KDa
SequenceString:
CACGUCCCUC UCCAAACGAG AGAACAUGCA UGGGCUGGCA UGAGCGCGUG GGGCCCCGAG GCGCUGAACC UUGAGGCCUC AUGCUGGGA CU

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Macromolecule #9: 60S ribosomal protein L18

MacromoleculeName: 60S ribosomal protein L18 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 21.595504 KDa
SequenceString: GVDLTGVQKK KRVVRHHTYS TNPYIKLLIK LYKFLAQRTN SAFNKLVHQR LLKSRSNRAP ISLSRIAVCM KRKSVWLEKG KKAPIAVIV GDVLDDVRMA RIPALRVCAL RFSKSARERI TGAGGECLTF DQLAMVAPTG KNTFLLRGRK SGRESVKHFG A AGVPGSHA ...String:
GVDLTGVQKK KRVVRHHTYS TNPYIKLLIK LYKFLAQRTN SAFNKLVHQR LLKSRSNRAP ISLSRIAVCM KRKSVWLEKG KKAPIAVIV GDVLDDVRMA RIPALRVCAL RFSKSARERI TGAGGECLTF DQLAMVAPTG KNTFLLRGRK SGRESVKHFG A AGVPGSHA KPFTSNRGKE RQRSSARRRA FRHK

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Macromolecule #10: 60S ribosomal protein L11

MacromoleculeName: 60S ribosomal protein L11 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 7.672055 KDa
SequenceString:
EIVVKKLCLN IVHCTVRTGS FGGMDFYVVL GRRGERVAHR RRKTSRVGCP HRVRKEEAMH WFERT

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Macromolecule #11: 60S ribosomal protein L13

MacromoleculeName: 60S ribosomal protein L13 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 23.690836 KDa
SequenceString: PKGKNAIPHV HQRKHWNPCS SQKGNVKVFL NQPAQKLRRR RLRLLKAKKT FPRPLKALRP QVNCPTVRHN MKKRLGRGFT VEELKAAGI NPRFAPTIGI RVDRRRKNKS EEGMSINIQR LKTYMSKLVL FPMSYKNVQK GEATEEEVKS ATQDRTRFGT A AVGGFVTP ...String:
PKGKNAIPHV HQRKHWNPCS SQKGNVKVFL NQPAQKLRRR RLRLLKAKKT FPRPLKALRP QVNCPTVRHN MKKRLGRGFT VEELKAAGI NPRFAPTIGI RVDRRRKNKS EEGMSINIQR LKTYMSKLVL FPMSYKNVQK GEATEEEVKS ATQDRTRFGT A AVGGFVTP APEAPRKVTE EERTKNVYKF LKKNHSAVRF FGIRRAR

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Macromolecule #12: 60S ribosomal protein L13a

MacromoleculeName: 60S ribosomal protein L13a / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 23.412801 KDa
SequenceString: HRPEIIVVDL KDHVLGRAAA IVAKQLLLGK KITAVRCEQL TIAGTEIRNK IKYLQFLRKR KLSNPKLGPF HHRSPSDIFL RTVRSMLPR YTKRGQKALR QLVAYEGIPT NVVRTGGRVV IPKAQRHYCY RSERPYTVLG NMCKHVGWKY SDVVKKLETA R VEKAARHH ...String:
HRPEIIVVDL KDHVLGRAAA IVAKQLLLGK KITAVRCEQL TIAGTEIRNK IKYLQFLRKR KLSNPKLGPF HHRSPSDIFL RTVRSMLPR YTKRGQKALR QLVAYEGIPT NVVRTGGRVV IPKAQRHYCY RSERPYTVLG NMCKHVGWKY SDVVKKLETA R VEKAARHH KKTEKLRAAW KSARKEALSK VSKNNLEVLK KFGYA

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Macromolecule #13: 40S ribosomal protein L14

MacromoleculeName: 40S ribosomal protein L14 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 17.483592 KDa
SequenceString:
NYIRAGRLVR IIRGPRQDRV GVVVDIIDGN RVLVENPADK KMWRHVQNLK NVEPLKFSVE LSRNCSTKTL KNVLAEKKIL EKYAATKSA RRIAAKRAFA RSTDFERYQL RVAKRSRAFW TRKVFDENDK KKPVSWHKVA LKKLQKNAKK

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Macromolecule #14: Ribosomal protein L15

MacromoleculeName: Ribosomal protein L15 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 24.154484 KDa
SequenceString: GAFMYLNELW KKKSSDVMRF IQRVRAWEFR HQHTVVRLRR PTRPEKARML GYKTKQGFSV FRVRVRRGGR KRPVHKGITY GKPKTSGVL GVKLNKNSRA IAEQRLGKKY GNLRVLNSYW VNADSTFVWY EVVAVDPMCR TIRRDPRINW IVNAVHKHRE Q RGLTSAGR ...String:
GAFMYLNELW KKKSSDVMRF IQRVRAWEFR HQHTVVRLRR PTRPEKARML GYKTKQGFSV FRVRVRRGGR KRPVHKGITY GKPKTSGVL GVKLNKNSRA IAEQRLGKKY GNLRVLNSYW VNADSTFVWY EVVAVDPMCR TIRRDPRINW IVNAVHKHRE Q RGLTSAGR KHRGLRHKGH KASKLRPSVR AAWRRNNRMV FLRKR

+
Macromolecule #15: 60S ribosomal protein L17

MacromoleculeName: 60S ribosomal protein L17 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 17.545625 KDa
SequenceString:
HYSRKPQVSS KSAKAKVSDL RCHFKNTFET ANVINGMPLR KAQQLYRQVL AKTRCIPFKR YNGKIGRTAQ AKEWGQTKGR WPRKSVVAM LSLLKNAEAN AIEKGLDPNK MVIKHVQVDQ AARMRRRTYR AHGRITPYMC SPCHVQLFMT QPQ

+
Macromolecule #16: 60S ribosomal protein L18a

MacromoleculeName: 60S ribosomal protein L18a / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 20.595014 KDa
SequenceString:
VKPHLRHYCV VGRETPSEKN PAPTVYKFEV FAPNFVVAKS RFWRMMREKN KVKSTHGDVL SCKVVKDRKL AARNYSVDIA YYSQRCGYT HMVKEFRDVS KAGAVSQAYH DLASRHRARY HNIEVLGVKS IPNHQVKRLS ISEYHASNLS FPLLHRRVKA P RKDRVVFV KKNTKRAVV

+
Macromolecule #17: Ribosomal protein L19-like protein

MacromoleculeName: Ribosomal protein L19-like protein / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 18.187561 KDa
SequenceString:
SLKLQARLAA DILRCGRHRV WLDPNEASEI SNANSRKSVR KLIKDGLIIR KPVKVHSRSR WRHMKEAKSM GRHEGAGRRE GTREARMPS KELWMRRLRI LRRLLRKYRE EKKIDRHIYR ELYVKAKGNV FRNKRNLMEH IHKVKNEKKK E

+
Macromolecule #18: Ribosomal protein L21E (60S)

MacromoleculeName: Ribosomal protein L21E (60S) / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 16.584541 KDa
SequenceString:
HSHGYKCGTR HLFAKKFRKH GVPSVSTILT NFKVGDYVDV VADSAVRAGM PHKYYHGRTG IVWNVTPRGV GVIVNKPVRN RTVRKRICV RFEHVRKSRC QEAFKQKQKD FLAFQAAKKA GKPLPPLKRS SRNGGIVRAK RVEVLAR

+
Macromolecule #19: 60S ribosomal protein L22

MacromoleculeName: 60S ribosomal protein L22 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 11.872974 KDa
SequenceString:
KVFKIDCTIP ANDGIFSEEI LGNFEQYFQE NVKLNGRKGK LTEKVRVGLR NNVLTITTTM AYRKKYFKYL TKKFLKKKDL RDWIRILAR GKDTYQLKYF

+
Macromolecule #20: 60S ribosomal protein L23

MacromoleculeName: 60S ribosomal protein L23 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 13.651026 KDa
SequenceString:
FRVSLALPVG AVVNCADNTG AKNLYIISVK GYHGRLNRLP AAALGDIVMA SVKKGKPELR RKVLNAVIIR QRKSWRRKDG TVIYFEDNA GVIVNPKGEM KGSGIAGPVA KEAADLWPKI SSHAPAIV

+
Macromolecule #21: 60S ribosomal protein L23a

MacromoleculeName: 60S ribosomal protein L23a / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 13.444673 KDa
SequenceString:
AYTRPQFRRP HTYRRPSIPK PSNNMSAISN KWDAFRVIRY PLTTDKAMKK IEENNTLTFI VDSNANKTEI KKAMRKLYQV KAVKVNTLI RPDGLKKAYI RLSASYDALE TANKMGL

+
Macromolecule #22: Ribosomal protein L24

MacromoleculeName: Ribosomal protein L24 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 7.363689 KDa
SequenceString:
TIDCEFSHFA VHPGHGRRYV PFAFLSTKPV LTFARPKCFA MYMRKKNPRF VPWTRTYRRI N

+
Macromolecule #23: 60S ribosomal protein L26

MacromoleculeName: 60S ribosomal protein L26 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 13.107445 KDa
SequenceString:
GSRRKARRAH FQAPSHVRRI LMSAPLSKEL RAKYNVRAMP VRKDDEVRVK RGAYKGREGK VTACYRLRWV IHIDKVNREK ANGTTVPVG VHPSNVEITK LKLNHNRKAI LERK

+
Macromolecule #24: Ribosomal protein L27

MacromoleculeName: Ribosomal protein L27 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 15.419276 KDa
SequenceString:
KFLKPGKVVI VTSGRYAGKK AVIVQNTDSK SKERPYGHSL IAGMKKYPKK VVRGMSKRTI QRRSRVGVFL RVVNHKHFLP TRYNMDLSR ELRGRINVSD ASKKAKSKQL VRKLFQARYN AGSNRWFFQR LRF

+
Macromolecule #25: 60S ribosomal protein L27A/L29

MacromoleculeName: 60S ribosomal protein L27A/L29 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 16.103848 KDa
SequenceString:
PTRFKKTRHQ RGSTFCGYGR VGKHRKHPSG RGNAGGQHHH RINFDKYHPG YFGKCGMNHY HLKKNPLWKP TINLNNLAKL IAKDEALKA KKGETLPVVD LLANGYSKLL GNGHLQTPCI VKARWVSKLA DKKIRKAGGA VVLQA

+
Macromolecule #26: 60S ribosomal protein L28

MacromoleculeName: 60S ribosomal protein L28 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 13.836174 KDa
SequenceString:
STDLQWLLVR QNSKFLQKRN GIRLSSDPFN NNANWTKRHA GFLNTKAAVV KTKGDRILVT TKDGKAGNKP KSMYKKAVMD AGVEASVVS KAVAAVRPDL ASIASRRARK MASTLEHMKK VRAARK

+
Macromolecule #27: Ribosomal protein L29

MacromoleculeName: Ribosomal protein L29 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 7.378556 KDa
SequenceString:
NHTNHNQSRK NHRNGIKRAL PMYLHNSKRG SWLPALINAR RVRKNNQKAA VKARRERLAA HKA

+
Macromolecule #28: 60S ribosomal protein L2

MacromoleculeName: 60S ribosomal protein L2 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 26.737242 KDa
SequenceString: GKTVLTCRKG NGSIYQVHGH KRLGPAKLRI LDYAERHGFM RGVVKTIEHE PGRGAPLARV EFRHPYKYRR VKELMVAPEG MFTGQSVLC GVKAPLAIGN VLPLGQITEG CIVCNVEAKV GDRGTIARAS GDYCIIISHN HETGRTRLKL PSGQKKTVPS N CRAMIGII ...String:
GKTVLTCRKG NGSIYQVHGH KRLGPAKLRI LDYAERHGFM RGVVKTIEHE PGRGAPLARV EFRHPYKYRR VKELMVAPEG MFTGQSVLC GVKAPLAIGN VLPLGQITEG CIVCNVEAKV GDRGTIARAS GDYCIIISHN HETGRTRLKL PSGQKKTVPS N CRAMIGII AGGGRIEKPV LKAGNSFYRF RGKRNCWPKV RGVARNPVEH PHGGGNHQHI GHPSTVSRHA PPGQKVGLIA AR RTGRI

+
Macromolecule #29: Ribosomal protein L13

MacromoleculeName: Ribosomal protein L13 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 45.359961 KDa
SequenceString: EHPRHGHLGF LPRKRSRQIR GRVRSFPKDD PSQKPHLTSF MVFKAGMTHI VRDVDRPGSK VNKKEVVEPV TILEAPPMVV VGIVGYRQT PVGHKTIGTV WAHHTSVEFR RRFYKNWKQS AQLAFTKRKQ FARTTEGRLA EARTLKAFAK KADIIRVVAH T QLRKLRNN ...String:
EHPRHGHLGF LPRKRSRQIR GRVRSFPKDD PSQKPHLTSF MVFKAGMTHI VRDVDRPGSK VNKKEVVEPV TILEAPPMVV VGIVGYRQT PVGHKTIGTV WAHHTSVEFR RRFYKNWKQS AQLAFTKRKQ FARTTEGRLA EARTLKAFAK KADIIRVVAH T QLRKLRNN RVGVKKAHVS EIQINGGTIA EKIELAKSLL EKEVRIDSIF QQSETCDVCA VTKGHGFTGV VKRWGVACLP RK THRGLRK VACIGAWHPA RVMYTVARAG QHGYHHRTHL NKKIYQLGRA VSMEPNQATT TYDLTAKSIT PMGGFVGYGT VRN DYIMLK GSVAGPRRRV ITLRRPMAPQ TSRKLTEQIT LKFIDTSSKI GHGRFQTKKE KSQWFGPLKK DRIRREERLR K

+
Macromolecule #30: 60S ribosomal protein L30

MacromoleculeName: 60S ribosomal protein L30 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 7.508954 KDa
SequenceString:
TKIQLVMKSG KYVLGTKQTL KTLRQGRSKL VVISSNCPPI RKAEIEYYCT LSKTPMHHYV LSITDV

+
Macromolecule #31: 60S ribosomal subunit protein L31

MacromoleculeName: 60S ribosomal subunit protein L31 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 19.48308 KDa
SequenceString:
ALLDARKKAI ASRKARDDRR WKRVLAKMDS EKRKKYHGVG NTAEHSRVRG ITRAALRKRT GRKPDNITME ATIHLSKLLK RKTFHKRAP IAIKRIKAFV ARLMKTKDNR IDASLNTYVW HKGVKGVPGR VRVHVERKSE TLEGGKRKHF YTVISNVPVA S FKNLTTKV T

+
Macromolecule #32: 60S ribosomal protein L32

MacromoleculeName: 60S ribosomal protein L32 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 13.218489 KDa
SequenceString:
VKKRTKRFTR HKCELFPQLS SSWRKPRGED SPVRRRYKGQ KAMPNKGYGS DRKTKYITPS GFKNFPIHNV QDLYMLLMQN RKYAGVISH TVGAKSRKAI VRKAHELDVR LING

+
Macromolecule #33: 60S ribosomal protein L34

MacromoleculeName: 60S ribosomal protein L34 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 12.302434 KDa
SequenceString:
PRVQYRRRMH YATRGNRMKL VRTPGNRLVM QKRVKRSQGP HTPWVLGHKR LAGTKALRHT EARLASRHEK STSRPYGGVL SHEQVRDRI VRAFLIEEQR IVKRA

+
Macromolecule #34: 60S ribosomal protein L35

MacromoleculeName: 60S ribosomal protein L35 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 14.526508 KDa
SequenceString:
IRDLRDKSKD ELLKQLMEFK KELSQLRVAQ QLNSAGTRLG RIRLIRKSIA RILTVLNRNQ RESLRKVYAG RRLRLAMPKA LRPKLTRRR RLALKDNEKN RKTRRQLRMA RKFPRRVYAV K

+
Macromolecule #35: Ribosomal protein L35A

MacromoleculeName: Ribosomal protein L35A / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 15.513169 KDa
SequenceString:
NRSKKIQQLT KKTSTVKRNR KSPRLYMKAV LAGYTRGLHG QNKNTAIVRI ENVNTKEDAA WYVGKRVCYV YHGYKMKRCV RWSKAPARR SNTRAIWGRV TRPHGGSGAV RAKFNGSAVP ASAIGKRVRV YLYPSRI

+
Macromolecule #36: Ribosomal protein L36

MacromoleculeName: Ribosomal protein L36 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 10.819966 KDa
SequenceString:
PAPRTGIIAG FNKGHKTTRR ARAPSSNDRY ALPHKKLRAV KAIITDLVGL SPMERRVQEL LRVGKDKRAL KFCKKRLGNF KAAKKRRAK MEEALR

+
Macromolecule #37: Ribosomal protein L37

MacromoleculeName: Ribosomal protein L37 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 9.734394 KDa
SequenceString:
TKGTTSMGQR HGRTHILCRR CGRNAYHVQW ERCAACAYPR ASRRRYNWSV KAIKRRRTGT GRMRYLKEVN RRIKNHFKTS L

+
Macromolecule #38: 60S ribosomal protein L37a

MacromoleculeName: 60S ribosomal protein L37a / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 9.609315 KDa
SequenceString:
KRTVKMGVMG RYGARYGANP RKRAKKLEVS QHARHFCSFC GKFAFRRKAV GIWRCDGCSK TMAGGAYTLS TPNNSTVRST VRRLR

+
Macromolecule #39: Ribosomal protein L38

MacromoleculeName: Ribosomal protein L38 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 6.840182 KDa
SequenceString:
PREIKNLKEF LAICSRKDAR CVKVKHNPAQ TKFKVRCSRY LYTLVVADKK KADKIERS

+
Macromolecule #40: Ribosomal protein L39

MacromoleculeName: Ribosomal protein L39 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 6.436773 KDa
SequenceString:
GRFKPLAIKK KYAKKLKQNR PVPYWIRLRT GNRIKWNEKR RHWRRTKLHY

+
Macromolecule #41: 60S ribosomal protein L4

MacromoleculeName: 60S ribosomal protein L4 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 37.228363 KDa
SequenceString: SVSVYSASED KVVGTCPLPA VFTAPIRHDI VQFVHTNMAK NSRQAYAVNR LSGMNHSAES WGTGRAVARI PRIGGGGTST SGAGAFGNM CRGGRMFAPT KIFRRWHRKI NLHQKRFAVV SALAASSVPA LVMSRGHKIE NVAEVPLVVE DAVQGYEKTK E AMAFLKAI ...String:
SVSVYSASED KVVGTCPLPA VFTAPIRHDI VQFVHTNMAK NSRQAYAVNR LSGMNHSAES WGTGRAVARI PRIGGGGTST SGAGAFGNM CRGGRMFAPT KIFRRWHRKI NLHQKRFAVV SALAASSVPA LVMSRGHKIE NVAEVPLVVE DAVQGYEKTK E AMAFLKAI GAVDDVNRVN DSREIRAGRG KMRNRRYVAR RGPMLVMPDN KGTRAFRNIF GLDLANVNAL NLLHLAPGGH VG RFVVWTK GAFEKLDSIF GTFTKASAVK KGFMLPAPML TNTDVTRIMQ SEEVRRVLKP KKLQPKKASR LTQPTNGFRN RRL RLRLNP FQKKESAMVK G

+
Macromolecule #42: 60S ribosomal protein L44

MacromoleculeName: 60S ribosomal protein L44 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 10.90806 KDa
SequenceString:
VNYPKKKTMY CPDARCKAHK SFKVVQYKAG KARLYARGKR RYDRKQSGYG GQTKPIFHKK AKTTKKIVLK LQCSNCKSII QNVLKRTKH FELN

+
Macromolecule #43: 60S ribosomal protein L5

MacromoleculeName: 60S ribosomal protein L5 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 28.665885 KDa
SequenceString: VVKNKAYFKR FQVKYRRRRE GKTDYQARRQ MVLQDKTKFG TPKYRLVVRI TNRDIIAQVV QAKVVGDEVV MAAYSHELPQ FGIQHGLTN YAAAYATGLL VARRMLTKLG LANKFEGTKE ADGSYSAVRT KADDQGDDEK RFPFKAILDV GLARTTTGAR V FGVMKGAV ...String:
VVKNKAYFKR FQVKYRRRRE GKTDYQARRQ MVLQDKTKFG TPKYRLVVRI TNRDIIAQVV QAKVVGDEVV MAAYSHELPQ FGIQHGLTN YAAAYATGLL VARRMLTKLG LANKFEGTKE ADGSYSAVRT KADDQGDDEK RFPFKAILDV GLARTTTGAR V FGVMKGAV DGGLAVPHRP NRFPGYDKEK DSLNAKAHRD RILGLHVAEY LKQVREEASS NPEESTCQFS KYIKDKVVPN EL EGMYKKA HAAIRAD

+
Macromolecule #44: 60S ribosomal protein L6

MacromoleculeName: 60S ribosomal protein L6 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 19.042512 KDa
SequenceString:
EYTTLRKSCK PGTIAIILAG RFRGRRVVIL KQLPRNGPLV ISGPMKYNGV PIRRIDSRYI IATSTKVDIK NVDVSGITPD IFKRPKQEK RVKSEGEFMG DKAKKAAEQK AKKTAKAAPK GIVSDARAQL QKKVDAALIE AIRKDPLGKE KAGYLHSVFT I KPGDAPHR MRW

+
Macromolecule #45: 60S ribosomal protein L7

MacromoleculeName: 60S ribosomal protein L7 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 24.898406 KDa
SequenceString: KAIAANKAAR VALKKAAYAR GLRYSREYRS AEKKLVHLRR QAKSCGNYYL EAKPKVAVVT RIRGIAKVAP KQRKILQLLR LRQIFNTVF VRLNKPMENM LRAVEPYIAY GYPSLRTVRM MVYKRGHLKI NGQRVKIVNN QMIESKYNNE NIVCAEDIVN Q IYTGGKHF ...String:
KAIAANKAAR VALKKAAYAR GLRYSREYRS AEKKLVHLRR QAKSCGNYYL EAKPKVAVVT RIRGIAKVAP KQRKILQLLR LRQIFNTVF VRLNKPMENM LRAVEPYIAY GYPSLRTVRM MVYKRGHLKI NGQRVKIVNN QMIESKYNNE NIVCAEDIVN Q IYTGGKHF REVTNGLWPF KLSPPAGGMR QKRRHFVEGG DYGNRDALIN RFLARMI

+
Macromolecule #46: Ribosomal protein L7a-like protein

MacromoleculeName: Ribosomal protein L7a-like protein / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Trypanosoma cruzi (eukaryote)
Molecular weightTheoretical: 25.701291 KDa
SequenceString: RPKNFGIGHD VPYARDLSRF MRWPTFVTMQ RKKRVLQRRL KVPPALHQFT KVLDRSSRNE LLKLVKKYPS ETRRARRQRL FDVATEKKK NPEAASKKAP LSVVTGLQEV TRTIEKKTAR LVMIANNVDP IELVLWMPTL CRANKVPYAI VKDKARLGDA V GRKTATCV ...String:
RPKNFGIGHD VPYARDLSRF MRWPTFVTMQ RKKRVLQRRL KVPPALHQFT KVLDRSSRNE LLKLVKKYPS ETRRARRQRL FDVATEKKK NPEAASKKAP LSVVTGLQEV TRTIEKKTAR LVMIANNVDP IELVLWMPTL CRANKVPYAI VKDKARLGDA V GRKTATCV AITDVNAEDE AALKNLIRSV NARFLARSDV IRRQWGGLQL SLRSRAELRK KRARN

+
Macromolecule #47: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 47 / Number of copies: 105 / Formula: MG
Molecular weightTheoretical: 24.305 Da

+
Macromolecule #48: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 48 / Number of copies: 3 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

+
Macromolecule #49: water

MacromoleculeName: water / type: ligand / ID: 49 / Number of copies: 83 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER / Water

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

BufferpH: 7.2
GridModel: Quantfoil / Material: COPPER / Mesh: 400
VitrificationCryogen name: ETHANE

-
Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
Image recordingFilm or detector model: FEI FALCON II (4k x 4k) / Average electron dose: 27.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: EMDB MAP
EMDB ID:

6315

Initial angle assignmentType: PROJECTION MATCHING
Final angle assignmentType: PROJECTION MATCHING
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.54 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 235000

-
Atomic model buiding 1

RefinementSpace: REAL / Overall B value: 56
Output model

PDB-5t5h:
Structure and assembly model for the Trypanosoma cruzi 60S ribosomal subunit

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Related info.:EMDB / PDB / SASBDB / Comparison of 3 databanks / Yorodumi Search / Aug 31, 2016. New EM Navigator & Yorodumi / Yorodumi Papers / Jmol/JSmol / Function and homology information / Changes in new EM Navigator and Yorodumi

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