EMDB-8308: Mediator-RNA Polymerase II Pre-Initiation Complex

Basic information

• Entry: Database: EMDB / ID: EMD-8308
• Title: Mediator-RNA Polymerase II Pre-Initiation Complex
• Map data: Mediator-RNA Polymerase II Pre-Initiation Complex

Sample

• Complex: Mediator-RNA Polymerase II Pre-Initiation Complex
  • Protein or peptide: x 52 types
  • DNA: x 1 types

• Biological species: yeast (fungus) / Saccharomyces cerevisiae (brewer's yeast)
• Method: single particle reconstruction / cryo EM / Resolution: 21.9 Å
• Authors: Robinson PJ / Bushnell DA / Kornberg RD

Funding support

United States, 5 items

Organization	Grant number	Country
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)	AI21144	United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)	GM36659	United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)	GM49985	United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)	GM103481	United States
National Science Foundation (NSF, United States)	1231306	United States

• Citation: Journal: Cell / Year: 2016; Title: Structure of a Complete Mediator-RNA Polymerase II Pre-Initiation Complex.; Authors: Philip J Robinson / Michael J Trnka / David A Bushnell / Ralph E Davis / Pierre-Jean Mattei / Alma L Burlingame / Roger D Kornberg / ; Abstract: A complete, 52-protein, 2.5 million dalton, Mediator-RNA polymerase II pre-initiation complex (Med-PIC) was assembled and analyzed by cryo-electron microscopy and by chemical cross-linking and mass spectrometry. The resulting complete Med-PIC structure reveals two components of functional significance, absent from previous structures, a protein kinase complex and the Mediator-activator interaction region. It thereby shows how the kinase and its target, the C-terminal domain of the polymerase, control Med-PIC interaction and transcription.

History

Deposition	Aug 4, 2016	-
Header (metadata) release	Sep 14, 2016	-
Map release	Sep 14, 2016	-
Update	Feb 3, 2021	-
Current status	Feb 3, 2021	Processing site: RCSB / Status: Released

Map

• File: Download / File: emd_8308.map.gz / Format: CCP4 / Size: 40.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: Mediator-RNA Polymerase II Pre-Initiation Complex
• Voxel size: X=Y=Z: 2.29 Å

Density

Contour Level	By AUTHOR: 0.025 / Movie #1: 0.025
Minimum - Maximum	-0.034128312 - 0.12879166
Average (Standard dev.)	0.0026270745 (±0.0121569345)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 220 220 220 Spacing 220 220 220
Cell	A=B=C: 503.8 Å α=β=γ: 90.0 °

CCP4 map header:

mode	Image stored as Reals
Å/pix. X/Y/Z	2.29	2.29	2.29
M x/y/z	220	220	220
origin x/y/z	0.000	0.000	0.000
length x/y/z	503.800	503.800	503.800
α/β/γ	90.000	90.000	90.000
start NX/NY/NZ	-210	-210	-210
NX/NY/NZ	420	420	420
MAP C/R/S	1	2	3
start NC/NR/NS	0	0	0
NC/NR/NS	220	220	220
D min/max/mean	-0.034	0.129	0.003

Supplemental data

Mask #1

• File: emd_8308_msk_1.map

Half map: Mediator-RNA Polymerase II Pre-Initiation Complex half-map odd

• File: emd_8308_half_map_1.map
• Annotation: Mediator-RNA Polymerase II Pre-Initiation Complex half-map odd

Half map: Mediator-RNA Polymerase II Pre-Initiation Complex half-map even

• File: emd_8308_half_map_2.map
• Annotation: Mediator-RNA Polymerase II Pre-Initiation Complex half-map even

Sample components

Entire : Mediator-RNA Polymerase II Pre-Initiation Complex

• Entire: Name: Mediator-RNA Polymerase II Pre-Initiation Complex

Components

• Complex: Mediator-RNA Polymerase II Pre-Initiation Complex
  • Protein or peptide: DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB1Polymerase
  • Protein or peptide: DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB2Polymerase
  • Protein or peptide: DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB3Polymerase
  • Protein or peptide: DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB4Polymerase
  • Protein or peptide: DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 1RNA polymerase
  • Protein or peptide: DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 2RNA polymerase
  • Protein or peptide: DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB7Polymerase
  • Protein or peptide: DNA-directed RNA polymerases I, II, and III subunit RPABC3RNA polymerase
  • Protein or peptide: DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB9Polymerase
  • Protein or peptide: DNA-directed RNA polymerases I, II, and III subunit RPABC5RNA polymerase
  • Protein or peptide: DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB11Polymerase
  • Protein or peptide: DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 4RNA polymerase
  • Protein or peptide: TRANSCRIPTION INITIATION FACTOR IIB
  • Protein or peptide: TATA-box-binding protein
  • Protein or peptide: Transcription factor TFIIE subunit TFA1
  • Protein or peptide: Transcription factor TFIIE subunit TFA2
  • Protein or peptide: Transcription initiation factor IIA large subunit
  • Protein or peptide: Transcription initiation factor IIA subunit 2
  • Protein or peptide: Transcription initiation factor IIF subunit alpha
  • Protein or peptide: Transcription initiation factor IIF subunit beta
  • Protein or peptide: Transcription initiation factor IIF subunit gamma
  • Protein or peptide: RNA polymerase II transcription factor B subunit 1
  • Protein or peptide: RNA polymerase II transcription factor B subunit 2
  • Protein or peptide: RNA polymerase II transcription factor B subunit 3
  • Protein or peptide: RNA polymerase II transcription factor B subunit 4
  • Protein or peptide: RNA polymerase II transcription factor B subunit 5
  • Protein or peptide: Serine/threonine-protein kinase KIN28
  • Protein or peptide: Cyclin CCL1
  • Protein or peptide: DNA repair helicase RAD3
  • Protein or peptide: Suppressor of stem-loop protein 1
  • Protein or peptide: DNA repair helicase RAD25
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 1
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 2
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 3
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 4
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 5
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 6
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 7
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 8
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 9
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 10
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 11
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 14
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 15
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 16
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 17
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 18
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 19
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 20
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 21
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 22
  • Protein or peptide: Mediator of RNA polymerase II transcription subunit 31
  • DNA: 108 bp HIS4 promoter fragment

Supramolecule #1: Mediator-RNA Polymerase II Pre-Initiation Complex

• Supramolecule: Name: Mediator-RNA Polymerase II Pre-Initiation Complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
• Molecular weight: Theoretical: 2.48 MDa

Macromolecule #1: DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB1

• Macromolecule: Name: DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB1 / type: protein_or_peptide / ID: 1 / Enantiomer: LEVO
• Source (natural): Organism: yeast (fungus)

Sequence

String:

MVGQQYSSAP LRTVKEVQFG LFSPEEVRAI SVAKIRFPET MDETQTRAKI GGLNDPRLGS IDRNLKCQTC QEGMNECPGH FGHIDLAKPV FHVGFIAKIK KVCECVCMHC GKLLLDEHNE LMRQALAIKD SKKRFAAIWT LCKTKMVCET DVPSEDDPTQ LVSRGGCGNT QPTIRKDGLK LVGSWKKDRA TGDADEPELR VLSTEEILNI FKHISVKDFT SLGFNEVFSR PEWMILTCLP VPPPPVRPSI SFNESQRGED DLTFKLADIL KANISLETLE HNGAPHHAIE EAESLLQFHV ATYMDNDIAG QPQALQKSGR PVKSIRARLK GKEGRIRGNL MGKRVDFSAR TVISGDPNLE LDQVGVPKSI AKTLTYPEVV TPYNIDRLTQ LVRNGPNEHP GAKYVIRDSG DRIDLRYSKR AGDIQLQYGW KVERHIMDND PVLFNRQPSL HKMSMMAHRV KVIPYSTFRL NLSVTSPYNA DFDGDEMNLH VPQSEETRAE LSQLCAVPLQ IVSPQSNKPC MGIVQDTLCG IRKLTLRDTF IELDQVLNML YWVPDWDGVI PTPAIIKPKP LWSGKQILSV AIPNGIHLQR FDEGTTLLSP KDNGMLIIDG QIIFGVVEKK TVGSSNGGLI HVVTREKGPQ VCAKLFGNIQ KVVNFWLLHN GFSTGIGDTI ADGPTMREIT ETIAEAKKKV LDVTKEAQAN LLTAKHGMTL RESFEDNVVR FLNEARDKAG RLAEVNLKDL NNVKQMVMAG SKGSFINIAQ MSACVGQQSV EGKRIAFGFV DRTLPHFSKD DYSPESKGFV ENSYLRGLTP QEFFFHAMGG REGLIDTAVK TAETGYIQRR LVKALEDIMV HYDNTTRNSL GNVIQFIYGE DGMDAAHIEK QSLDTIGGSD AAFEKRYRVD LLNTDHTLDP SLLESGSEIL GDLKLQVLLD EEYKQLVKDR KFLREVFVDG EANWPLPVNI RRIIQNAQQT FHIDHTKPSD LTIKDIVLGV KDLQENLLVL RGKNEIIQNA QRDAVTLFCC LLRSRLATRR VLQEYRLTKQ AFDWVLSNIE AQFLRSVVHP GEMVGVLAAQ SIGEPATQMT LNTFHFAGVA SKKVTSGVPR LKEILNVAKN MKTPSLTVYL EPGHAADQEQ AKLIRSAIEH TTLKSVTIAS EIYYDPDPRS TVIPEDEEII QLHFSLLDEE AEQSFDQQSP WLLRLELDRA AMNDKDLTMG QVGERIKQTF KNDLFVIWSE DNDEKLIIRC RVVRPKSLDA ETEAEEDHML KKIENTMLEN ITLRGVENIE RVVMMKYDRK VPSPTGEYVK EPEWVLETDG VNLSEVMTVP GIDPTRIYTN SFIDIMEVLG IEAGRAALYK EVYNVIASDG SYVNYRHMAL LVDVMTTQGG LTSVTRHGFN RSNTGALMRC SFEETVEILF EAGASAELDD CRGVSENVIL GQMAPIGTGA FDVMIDEESL VKYMPEQKIT EIEDGQDGGV TPYSNESGLV NADLDVKDEL MFSPLVDSGS NDAMAGGFTA YGGADYGEAT SPFGAYGEAP TSPGFGVSSP GFSPTSPTYS PTSPAYSPTS PSYSPTSPSY SPTSPSYSPT SPSYSPTSPS YSPTSPSYSP TSPSYSPTSP SYSPTSPSYS PTSPSYSPTS PSYSPTSPSY SPTSPSYSPT SPSYSPTSPA YSPTSPSYSP TSPSYSPTSP SYSPTSPSYS PTSPNYSPTS PSYSPTSPGY SPGSPAYSPK QDEQKHNENE NSR

Macromolecule #2: DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB2

• Macromolecule: Name: DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB2 / type: protein_or_peptide / ID: 2 / Enantiomer: LEVO
• Source (natural): Organism: yeast (fungus)

Sequence

String:

MSDLANSEKY YDEDPYGFED ESAPITAEDS WAVISAFFRE KGLVSQQLDS FNQFVDYTLQ DIICEDSTLI LEQLAQHTTE SDNISRKYEI SFGKIYVTKP MVNESDGVTH ALYPQEARLR NLTYSSGLFV DVKKRTYEAI DVPGRELKYE LIAEESEDDS ESGKVFIGRL PIMLRSKNCY LSEATESDLY KLKECPFDMG GYFIINGSEK VLIAQERSAG NIVQVFKKAA PSPISHVAEI RSALEKGSRF ISTLQVKLYG REGSSARTIK ATLPYIKQDI PIVIIFRALG IIPDGEILEH ICYDVNDWQM LEMLKPCVED GFVIQDRETA LDFIGRRGTA LGIKKEKRIQ YAKDILQKEF LPHITQLEGF ESRKAFFLGY MINRLLLCAL DRKDQDDRDH FGKKRLDLAG PLLAQLFKTL FKKLTKDIFR YMQRTVEEAH DFNMKLAINA KTITSGLKYA LATGNWGEQK KAMSSRAGVS QVLNRYTYSS TLSHLRRTNT PIGRDGKLAK PRQLHNTHWG LVCPAETPEG QACGLVKNLS LMSCISVGTD PMPIITFLSE WGMEPLEDYV PHQSPDATRV FVNGVWHGVH RNPARLMETL RTLRRKGDIN PEVSMIRDIR EKELKIFTDA GRVYRPLFIV EDDESLGHKE LKVRKGHIAK LMATEYQDIE GGFEDVEEYT WSSLLNEGLV EYIDAEEEES ILIAMQPEDL EPAEANEEND LDVDPAKRIR VSHHATTFTH CEIHPSMILG VAASIIPFPD HNQSPRNTYQ SAMGKQAMGV FLTNYNVRMD TMANILYYPQ KPLGTTRAME YLKFRELPAG QNAIVAIACY SGYNQEDSMI MNQSSIDRGL FRSLFFRSYM DQEKKYGMSI TETFEKPQRT NTLRMKHGTY DKLDDDGLIA PGVRVSGEDV IIGKTTPISP DEEELGQRTA YHSKRDASTP LRSTENGIVD QVLVTTNQDG LKFVKVRVRT TKIPQIGDKF ASRHGQKGTI GITYRREDMP FTAEGIVPDL IINPHAIPSR MTVAHLIECL LSKVAALSGN EGDASPFTDI TVEGISKLLR EHGYQSRGFE VMYNGHTGKK LMAQIFFGPT YYQRLRHMVD DKIHARARGP MQVLTRQPVE GRSRDGGLRF GEMERDCMIA HGAASFLKER LMEASDAFRV HICGICGLMT VIAKLNHNQF ECKGCDNKID IYQIHIPYAA KLLFQELMAM NITPRLYTDR SRDF

Macromolecule #3: DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB3

• Macromolecule: Name: DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB3 / type: protein_or_peptide / ID: 3 / Enantiomer: LEVO
• Source (natural): Organism: yeast (fungus)

Sequence

String:

MSEEGPQVKI REASKDNVDF ILSNVDLAMA NSLRRVMIAE IPTLAIDSVE VETNTTVLAD EFIAHRLGLI PLQSMDIEQL EYSRDCFCED HCDKCSVVLT LQAFGESEST TNVYSKDLVI VSNLMGRNIG HPIIQDKEGN GVLICKLRKG QELKLTCVAK KGIAKEHAKW GPAAAIEFEY DPWNKLKHTD YWYEQDSAKE WPQSKNCEYE DPPNEGDPFD YKAQADTFYM NVESVGSIPV DQVVVRGIDT LQKKVASILL ALTQMDQDKV NFASGDNNTA SNMLGSNEDV MMTGAEQDPY SNASQMGNTG SGGYDNAW

Macromolecule #4: DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB4

• Macromolecule: Name: DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB4 / type: protein_or_peptide / ID: 4 / Enantiomer: LEVO
• Source (natural): Organism: yeast (fungus)

Sequence

String:

MNVSTSTFQT RRRRLKKVEE EENAATLQLG QEFQLKQINH QGEEEELIAL NLSEARLVIK EALVERRRAF KRSQKKHKKK HLKHENANDE TTAVEDEDDD LDEDDVNADD DDFMHSETRE KELESIDVLL EQTTGGNNKD LKNTMQYLTN FSRFRDQETV GAVIQLLKST GLHPFEVAQL GSLACDTADE AKTLIPSLNN KISDDELERI LKELSNLETL Y

Macromolecule #5: DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 1

• Macromolecule: Name: DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 1; type: protein_or_peptide / ID: 5 / Enantiomer: LEVO
• Source (natural): Organism: yeast (fungus)

Sequence

String:

MDQENERNIS RLWRAFRTVK EMVKDRGYFI TQEEVELPLE DFKAKYCDSM GRPQRKMMSF QANPTEESIS KFPDMGSLWV EFCDEPSVGV KTMKTFVIHI QEKNFQTGIF VYQNNITPSA MKLVPSIPPA TIETFNEAAL VVNITHHELV PKHIRLSSDE KRELLKRYRL KESQLPRIQR ADPVALYLGL KRGEVVKIIR KSETSGRYAS YRICM

Macromolecule #6: DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 2

• Macromolecule: Name: DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 2; type: protein_or_peptide / ID: 6 / Enantiomer: LEVO
• Source (natural): Organism: yeast (fungus)

Sequence

String:

MSDYEEAFND GNENFEDFDV EHFSDEETYE EKPQFKDGET TDANGKTIVT GGNGPEDFQQ HEQIRRKTLK EKAIPKDQRA TTPYMTKYER ARILGTRALQ ISMNAPVFVD LEGETDPLRI AMKELAEKKI PLVIRRYLPD GSFEDWSVEE LIVDL

Macromolecule #7: DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB7

• Macromolecule: Name: DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB7 / type: protein_or_peptide / ID: 7 / Enantiomer: LEVO
• Source (natural): Organism: yeast (fungus)

Sequence

String:

MFFIKDLSLN ITLHPSFFGP RMKQYLKTKL LEEVEGSCTG KFGYILCVLD YDNIDIQRGR ILPTDGSAEF NVKYRAVVFK PFKGEVVDGT VVSCSQHGFE VQVGPMKVFV TKHLMPQDLT FNAGSNPPSY QSSEDVITIK SRIRVKIEGC ISQVSSIHAI GSIKEDYLGA I

Macromolecule #8: DNA-directed RNA polymerases I, II, and III subunit RPABC3

• Macromolecule: Name: DNA-directed RNA polymerases I, II, and III subunit RPABC3; type: protein_or_peptide / ID: 8 / Enantiomer: LEVO
• Source (natural): Organism: yeast (fungus)

Sequence

String:

MSNTLFDDIF QVSEVDPGRY NKVCRIEAAS TTQDQCKLTL DINVELFPVA AQDSLTVTIA SSLNLEDTPA NDSSATRSWR PPQAGDRSLA DDYDYVMYGT AYKFEEVSKD LIAVYYSFGG LLMRLEGNYR NLNNLKQENA YLLIRR

Macromolecule #9: DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB9

• Macromolecule: Name: DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB9 / type: protein_or_peptide / ID: 9 / Enantiomer: LEVO
• Source (natural): Organism: yeast (fungus)

Sequence

String:

MTTFRFCRDC NNMLYPREDK ENNRLLFECR TCSYVEEAGS PLVYRHELIT NIGETAGVVQ DIGSDPTLPR SDRECPKCHS RENVFFQSQQ RRKDTSMVLF FVCLSCSHIF TSDQKNKRTQ FS

Macromolecule #10: DNA-directed RNA polymerases I, II, and III subunit RPABC5

• Macromolecule: Name: DNA-directed RNA polymerases I, II, and III subunit RPABC5; type: protein_or_peptide / ID: 10 / Enantiomer: LEVO
• Source (natural): Organism: yeast (fungus)

Sequence

String:

MIVPVRCFSC GKVVGDKWES YLNLLQEDEL DEGTALSRLG LKRYCCRRMI LTHVDLIEKF LRYNPLEKRD

Macromolecule #11: DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB11

• Macromolecule: Name: DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB11 / type: protein_or_peptide / ID: 11 / Enantiomer: LEVO
• Source (natural): Organism: yeast (fungus)

Sequence

String:

MNAPDRFELF LLGEGESKLK IDPDTKAPNA VVITFEKEDH TLGNLIRAEL LNDRKVLFAA YKVEHPFFAR FKLRIQTTEG YDPKDALKNA CNSIINKLGA LKTNFETEWN LQTLAADDAF

Macromolecule #12: DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 4

• Macromolecule: Name: DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC 4; type: protein_or_peptide / ID: 12 / Enantiomer: LEVO
• Source (natural): Organism: yeast (fungus)

Sequence

String:

MSREGFQIPT NLDAAAAGTS QARTATLKYI CAECSSKLSL SRTDAVRCKD CGHRILLKAR TKRLVQFEAR

Macromolecule #13: TRANSCRIPTION INITIATION FACTOR IIB

• Macromolecule: Name: TRANSCRIPTION INITIATION FACTOR IIB / type: protein_or_peptide / ID: 13 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

MMTRESIDKR AGRRGPNLNI VLTCPECKVY PPKIVERFSE GDVVCALCGL VLSDKLVDTR SEWRTFSNDD HNGDDPSRVG EASNPLLDGN NLSTRIGKGE TTDMRFTKEL NKAQGKNVMD KKDNEVQAAF AKITMLCDAA ELPKIVKDCA KEAYKLCHDE KTLKGKSMES IMAASILIGC RRAEVARTFK EIQSLIHVKT KEFGKTLNIM KNILRGKSED GFLKIDTDNM SGAQNLTYIP RFCSHLGLPM QVTTSAEYTA KKCKEIKEIA GKSPITIAVV SIYLNILLFQ IPITAAKVGQ TLQVTEGTIK SGYKILYEHR DKLVDPQLIA NGVVSLDNLP GVEKK

Macromolecule #14: TATA-box-binding protein

• Macromolecule: Name: TATA-box-binding protein / type: protein_or_peptide / ID: 14 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

MADEERLKEF KEANKIVFDP NTRQVWENQN RDGTKPATTF QSEEDIKRAA PESEKDTSAT SGIVPTLQNI VATVTLGCRL DLKTVALHAR NAEYNPKRFA AVIMRIREPK TTALIFASGK MVVTGAKSED DSKLASRKYA RIIQKIGFAA KFTDFKIQNI VGSCDVKFPI RLEGLAFSHG TFSSYEPELF PGLIYRMVKP KIVLLIFVSG KIVLTGAKQR EEIYQAFEAI YPVLSEFRKM

Macromolecule #15: Transcription factor TFIIE subunit TFA1

• Macromolecule: Name: Transcription factor TFIIE subunit TFA1 / type: protein_or_peptide / ID: 15 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

MDRPIDDIVK NLLKFVVRGF YGGSFVLVLD AILFHSVLAE DDLKQLLSIN KTELGPLIAR LRSDRLISIH KQREYPPNSK SVERVYYYVK YPHAIDAIKW KVHQVVQRLK DDLDKNSEPN GYMCPICLTK YTQLEAVQLL NFDRTEFLCS LCDEPLVEDD SGKKNKEKQD KLNRLMDQIQ PIIDSLKKID DSRIEENTFE IALARLIPPQ NQSHAAYTYN PKKGSTMFRP GDSAPLPNLM GTALGNDSSR RAGANSQATL HINITTASDE VAQRELQERQ AEEKRKQNAV PEWHKQSTIG KTALGRLDNE EEFDPVVTAS AMDSINPDNE PAQETSYQNN RTLTEQEMEE RENEKTLNDY YAALAKKQAK LNKEEEEEEE EEEDEEEEEE EEMEDVMDDN DETARENALE DEFEDVTDTA GTAKTESNTS NDVKQESIND KTEDAVNATA TASGPSANAK PNDGDDDDDD DDDEMDIEFE DV

Macromolecule #16: Transcription factor TFIIE subunit TFA2

• Macromolecule: Name: Transcription factor TFIIE subunit TFA2 / type: protein_or_peptide / ID: 16 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

MSKNRDPLLA NLNAFKSKVK SAPVIAPAKV GQKKTNDTVI TIDGNTRKRT ASERAQENTL NSAKNPVLVD IKKEAGSNSS NAISLDDDDD DEDFGSSPSK KVRPGSIAAA ALQANQTDIS KSHDSSKLLW ATEYIQKKGK PVLVNELLDY LSMKKDDKVI ELLKKLDRIE FDPKKGTFKY LSTYDVHSPS ELLKLLRSQV TFKGISCKDL KDGWPQCDET INQLEEDSKI LVLRTKKDKT PRYVWYNSGG NLKCIDEEFV KMWENVQLPQ FAELPRKLQD LGLKPASVDP ATIKRQTKRV EVKKKRQRKG KITNTHMTGI LKDYSHRV

Macromolecule #17: Transcription initiation factor IIA large subunit

• Macromolecule: Name: Transcription initiation factor IIA large subunit / type: protein_or_peptide / ID: 17 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

MSNAEASRVY EIIVESVVNE VREDFENAGI DEQTLQDLKN IWQKKLTETK VTTFSWDNQF NEGNINGVQN DLNFNLATPG VNSSEFNIKE ENTGNEGLIL PNINSNNNIP HSGETNINTN TVEATNNSGA TLNTNTSGNT NADVTSQPKI EVKPEIELTI NNANITTVEN IDDESEKKDD EEKEEDVEKT RKEKEQIEQV KLQAKKEKRS ALLDTDEVGS ELDDSDDDYL ISEGEEDGPD ENLMLCLYDK VTRTKARWKC SLKDGVVTIN RNDYTFQKAQ VEAEWV

Macromolecule #18: Transcription initiation factor IIA subunit 2

• Macromolecule: Name: Transcription initiation factor IIA subunit 2 / type: protein_or_peptide / ID: 18 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

MAVPGYYELY RRSTIGNSLV DALDTLISDG RIEASLAMRV LETFDKVVAE TLKDNTQSKL TVKGNLDTYG FCDDVWTFIV KNCQVTVEDS HRDASQNGSG DSQSVISVDK LRIVACNSKK SE

Macromolecule #19: Transcription initiation factor IIF subunit alpha

• Macromolecule: Name: Transcription initiation factor IIF subunit alpha / type: protein_or_peptide / ID: 19 / Enantiomer: LEVO
• Source (natural): Organism: yeast (fungus)

Sequence

String:

MSRRNPPGSR NGGGPTNASP FIKRDRMRRN FLRMRMGQNG SNSSSPGVPN GDNSRGSLVK KDDPEYAEER EKMLLQIGVE ADAGRSNVKV KDEDPNEYNE FPLRAIPKED LENMRTHLLK FQSKKKINPV TDFHLPVRLH RKDTRNLQFQ LTRAEIVQRQ KEISEYKKKA EQERSTPNSG GMNKSGTVSL NNTVKDGSQT PTVDSVTKDN TANGVNSSIP TVTGSSVPPA SPTTVSAVES NGLSNGSTSA ANGLDGNAST ANLANGRPLV TKLEDAGPAE DPTKVGMVKY DGKEVTNEPE FEEGTMDPLA DVAPDGGGRA KRGNLRRKTR QLKVLDENAK KLRFEEFYPW VMEDFDGYNT WVGSYEAGNS DSYVLLSVED DGSFTMIPAD KVYKFTARNK YATLTIDEAE KRMDKKSGEV PRWLMKHLDN IGTTTTRYDR TRRKLKAVAD QQAMDEDDRD DNSEVELDYD EEFADDEEAP IIDGNEQENK ESEQRIKKEM LQANAMGLRD EEAPSENEED ELFGEKKIDE DGERIKKALQ KTELAALYSS DENEINPYLS ESDIENKENE SPVKKEEDSD TLSKSKRSSP KKQQKKATNA HVHKEPTLRV KSIKNCVIIL KGDKKILKSF PEGEWNPQTT KAVDSSNNAS NTVPSPIKQE EGLNSTVAER EETPAPTITE KDIIEAIGDG KVNIKEFGKF IRRKYPGAEN KKLMFAIVKK LCRKVGNDHM ELKKE

Macromolecule #20: Transcription initiation factor IIF subunit beta

• Macromolecule: Name: Transcription initiation factor IIF subunit beta / type: protein_or_peptide / ID: 20 / Enantiomer: LEVO
• Source (natural): Organism: yeast (fungus)

Sequence

String:

MSSGSAGAPA LSNNSTNSVA KEKSGNISGD EYLSQEEEVF DGNDIENNET KVYEESLDLD LERSNRQVWL VRLPMFLAEK WRDRNNLHGQ ELGKIRINKD GSKITLLLNE NDNDSIPHEY DLELTKKVVE NEYVFTEQNL KKYQQRKKEL EADPEKQRQA YLKKQEREEE LKKKQQQQKR RNNRKKFNHR VMTDRDGRDR YIPYVKTIPK KTAIVGTVCH ECQVMPSMND PNYHKIVEQR RNIVKLNNKE RITTLDETVG VTMSHTGMSM RSDNSNFLKV GREKAKSNIK SIRMPKKEIL DYLFKLFDEY DYWSLKGLKE RTRQPEAHLK ECLDKVATLV KKGPYAFKYT LRPEYKKLKE EERKATLGEL ADEQTGSAGD NAQGDAEADL EDEIEMEDVV

Macromolecule #21: Transcription initiation factor IIF subunit gamma

• Macromolecule: Name: Transcription initiation factor IIF subunit gamma / type: protein_or_peptide / ID: 21 / Enantiomer: LEVO
• Source (natural): Organism: yeast (fungus)

Sequence

String:

MVATVKRTIR IKTQQHILPE VPPVENFPVR QWSIEIVLLD DEGKEIPATI FDKVIYHLHP TFANPNRTFT DPPFRIEEQG WGGFPLDISV FLLEKAGERK IPHDLNFLQE SYEVEHVIQI PLNKPLLTEE LAKSGSTEET TANTGTIGKR RTTTNTTAEP KAKRAKTGSA STVKGSVDLE KLAFGLTKLN EDDLVGVVQM VTDNKTPEMN VTNNVEEGEF IIDLYSLPEG LLKSLWDYVK KNTE

Macromolecule #22: RNA polymerase II transcription factor B subunit 1

• Macromolecule: Name: RNA polymerase II transcription factor B subunit 1 / type: protein_or_peptide / ID: 22 / Enantiomer: LEVO
• Source (natural): Organism: yeast (fungus)

Sequence

String:

MSHSGAAIFE KVSGIIAINE DVSPAELTWR STDGDKVHTV VLSTIDKLQA TPASSEKMML RLIGKVDESK KRKDNEGNEV VPKPQRHMFS FNNRTVMDNI KMTLQQIISR YKDADIYEEK RRREESAQHT ETPMSSSSVT AGTPTPHLDT PQLNNGAPLI NTAKLDDSLS KEKLLTNLKL QQSLLKGNKV LMKVFQETVI NAGLPPSEFW STRIPLLRAF ALSTSQKVGP YNVLSTIKPV ASSENKVNVN LSREKILNIF ENYPIVKKAY TDNVPKNFKE PEFWARFFSS KLFRKLRGEK IMQNDRGDVI IDRYLTLDQE FDRKDDDMLL HPVKKIIDLD GNIQDDPVVR GNRPDFTMQP GVDINGNSDG TVDILKGMNR LSEKMIMALK NEYSRTNLQN KSNITNDEED EDNDERNELK IDDLNESYKT NYAIIHLKRN AHEKTTDNDA KSSADSIKNA DLKVSNQQML QQLSLVMDNL INKLDLNQVV PNNEVSNKIN KRVITAIKIN AKQAKHNNVN SALGSFVDNT SQANELEVKS TLPIDLLESC RMLHTTCCEF LKHFYIHFQS GEQKQASTVK KLYNHLKDCI EKLNELFQDV LNGDGESMSN TCTAYLKPVL NSITLATHKY DEYFNEYNNN SN

Macromolecule #23: RNA polymerase II transcription factor B subunit 2

• Macromolecule: Name: RNA polymerase II transcription factor B subunit 2 / type: protein_or_peptide / ID: 23 / Enantiomer: LEVO
• Source (natural): Organism: yeast (fungus)

Sequence

String:

MSDYSLKHSV TQYLEEIPQQ VQNRLYTSPA TCLAIYRILP PLAKFFIMAM VFNENEVPLL DLDKWVNSNG KLQFQNAIKS MKSLHLLIPN KSSGTLMINL NPTFKISLRN ALTGGEVQNS FGVVVEENVV SLDLLDEYSA NKWETILHFM VGTPLAKIPS EKVLNLLKHS KLMEEVNSTG EFKITNEGFQ FLLQEINSQL WTLLLQYLKM IETSKMDLVD VLHFIFMLGA LEVGKAYKID ALSETQRIML QDMRDYGLVF QKHSNDSIFY PTKLALMLTS DTKTIRSASN AMDSVLRQNR EEPSVNEDGA NGKSTTDITT SDDLNKAGLK NQDIPDGSLI VETNFKIYSY SNSPLQIAVL SLFVHLKARF VNMVLGQITR ESIRRALTNG ITADQIIAYL ETHAHPQMRR LAEEKLEKKL ELDPNCKEPL QVLPPTVVDQ IRLWQLELDR VITYEGSLYS DFETSQEYNL LSKYAQDIGV LLWKDDKKKK FFISKEGNSQ VLDFAKRKLK KKQ

Macromolecule #24: RNA polymerase II transcription factor B subunit 3

• Macromolecule: Name: RNA polymerase II transcription factor B subunit 3 / type: protein_or_peptide / ID: 24 / Enantiomer: LEVO
• Source (natural): Organism: yeast (fungus)

Sequence

String:

MLMDEYEENK DMCPICKTDR YLSPDVKFLV NPECYHRICE SCVDRIFSLG PAQCPYKGCD KILRKNKFKT QIFDDVEVEK EVDIRKRVFN VFNKTIDDFN GDLVEYNKYL EEVEDIIYKL DHGIDVAKTE EKLRTYEELN KQLIMNNLER SRTEIESFEQ RQKFEKEMKL KKRLLERQIE EEERMNKEWT KKEIVNRLST TTQDINETIE GVKNTVKLKK SSARRKLEEL NRVLKNNPYF NSNVNVQNSR LKDAVPFTPF NGDREAHPRF TLKGSVYNDP FIKDLEHRKE FIASGFNTNY AYERVLTEAF MGLGCVISEE L

Macromolecule #25: RNA polymerase II transcription factor B subunit 4

• Macromolecule: Name: RNA polymerase II transcription factor B subunit 4 / type: protein_or_peptide / ID: 25 / Enantiomer: LEVO
• Source (natural): Organism: yeast (fungus)

Sequence

String:

MDAISDPTFK HARSRKQVTE ESPSLLTVII EIAPKLWTTF DEEGNEKGSI IKVLEALIVF LNAHLAFNSA NKVAVIAAYS QGIKYLYPES TSALKASESE NKTRSDLKII NSDMYRRFRN VDETLVEEIY KLFELEKKQI EQNSQRSTLA GAMSAGLTYV NRISKESVTT SLKSRLLVLT CGSGSSKDEI FQYIPIMNCI FSATKMKCPI DVVKIGGSKE STFLQQTTDA TNGVYLHVES TEGLIQYLAT AMFIDPSLRP IIVKPNHGSV DFRTSCYLTG RVVAVGFICS VCLCVLSIIP PGNKCPACDS QFDEHVIAKL KRKPVVPRLK AKKKVTKP

Macromolecule #26: RNA polymerase II transcription factor B subunit 5

• Macromolecule: Name: RNA polymerase II transcription factor B subunit 5 / type: protein_or_peptide / ID: 26 / Enantiomer: LEVO
• Source (natural): Organism: yeast (fungus)

Sequence

String:

MARARKGALV QCDPSIKALI LQIDAKMSDI VLEELDDTHL LVNPSKVEFV KHELNRLLSK NIYNPMDEEE NQ

Macromolecule #27: Serine/threonine-protein kinase KIN28

• Macromolecule: Name: Serine/threonine-protein kinase KIN28 / type: protein_or_peptide / ID: 27 / Enantiomer: LEVO
• Source (natural): Organism: yeast (fungus)

Sequence

String:

MKVNMEYTKE KKVGEGTYAV VYLGCQHSTG RKIAIKEIKT SEFKDGLDMS AIREVKYLQE MQHPNVIELI DIFMAYDNLN LVLEFLPTDL EVVIKDKSIL FTPADIKAWM LMTLRGVYHC HRNFILHRDL KPNNLLFSPD GQIKVADFGL ARAIPAPHEI LTSNVVTRWY RAPELLFGAK HYTSAIDIWS VGVIFAELML RIPYLPGQND VDQMEVTFRA LGTPTDRDWP EVSSFMTYNK LQIYPPPSRD ELRKRFIAAS EYALDFMCGM LTMNPQKRWT AVQCLESDYF KELPPPSDPS SIKIRN

Macromolecule #28: Cyclin CCL1

• Macromolecule: Name: Cyclin CCL1 / type: protein_or_peptide / ID: 28 / Enantiomer: LEVO
• Source (natural): Organism: yeast (fungus)

Sequence

String:

MTDIQLNGKS TLDTPSATMS AKEKEAKLKS ADENNKPPNY KRISDDDLYR HSSQYRMWSY TKDQLQEKRV DTNARAIAYI EENLLKFREA HNLTEEEIKV LEAKAIPLTM EEELDLVNFY AKKVQVIAQH LNLPTEVVAT AISFFRRFFL ENSVMQIDPK SIVHTTIFLA CKSENYFISV DSFAQKAKST RDSVLKFEFK LLESLKFSLL NHHPYKPLHG FFLDIQNVLY GKVDLNYMGQ IYDRCKKRIT AALLTDVVYF YTPPQITLAT LLIEDEALVT RYLETKFPSR EGSQESVPGN EKEEPQNDAS TTEKNKEKST ESEEYSIDSA KLLTIIRECK SIIEDCKPPS TEEAKKIAAK NYYCQNPSTL IQKLKRKLNG EDTSSTVEKK QKT

Macromolecule #29: DNA repair helicase RAD3

• Macromolecule: Name: DNA repair helicase RAD3 / type: protein_or_peptide / ID: 29 / Enantiomer: LEVO
• Source (natural): Organism: yeast (fungus)

Sequence

String:

MKFYIDDLPV LFPYPKIYPE QYNYMCDIKK TLDVGGNSIL EMPSGTGKTV SLLSLTIAYQ MHYPEHRKII YCSRTMSEIE KALVELENLM DYRTKELGYQ EDFRGLGLTS RKNLCLHPEV SKERKGTVVD EKCRRMTNGQ AKRKLEEDPE ANVELCEYHE NLYNIEVEDY LPKGVFSFEK LLKYCEEKTL CPYFIVRRMI SLCNIIIYSY HYLLDPKIAE RVSNEVSKDS IVIFDEAHNI DNVCIESLSL DLTTDALRRA TRGANALDER ISEVRKVDSQ KLQDEYEKLV QGLHSADILT DQEEPFVETP VLPQDLLTEA IPGNIRRAEH FVSFLKRLIE YLKTRMKVLH VISETPKSFL QHLKQLTFIE RKPLRFCSER LSLLVRTLEV TEVEDFTALK DIATFATLIS TYEEGFLLII EPYEIENAAV PNPIMRFTCL DASIAIKPVF ERFSSVIITS GTISPLDMYP RMLNFKTVLQ KSYAMTLAKK SFLPMIITKG SDQVAISSRF EIRNDPSIVR NYGSMLVEFA KITPDGMVVF FPSYLYMESI VSMWQTMGIL DEVWKHKLIL VETPDAQETS LALETYRKAC SNGRGAILLS VARGKVSEGI DFDHQYGRTV LMIGIPFQYT ESRILKARLE FMRENYRIRE NDFLSFDAMR HAAQCLGRVL RGKDDYGVMV LADRRFSRKR SQLPKWIAQG LSDADLNLST DMAISNTKQF LRTMAQPTDP KDQEGVSVWS YEDLIKHQNS RKDQGGFIEN ENKEGEQDED EDEDIEMQ

Macromolecule #30: Suppressor of stem-loop protein 1

• Macromolecule: Name: Suppressor of stem-loop protein 1 / type: protein_or_peptide / ID: 30 / Enantiomer: LEVO
• Source (natural): Organism: yeast (fungus)

Sequence

String:

MAPVVISESE EDEDRVAITR RTKRQVHFDG EGDDRVDQQQ QQHSSSHRDR DKHVQRKKKK RLSNRNLQGS NGGYAWEDEI KRSWDLVKVD DEGDMASLVA SIVEARKKRT AKKNITPYQR GIIRSLILTL DCSEAMLEKD LRPNRHAMII QYAIDFVHEF FDQNPISQMG IIIMRNGLAQ LVSQVSGNPQ DHIDALKSIR KQEPKGNPSL QNALEMARGL LLPVPAHCTR EVLIVFGSLS TTDPGDIHQT IDSLVSEKIR VKVLGLSAQV AICKELCKAT NYGDESFYKI LLDETHLKEL FNEAVTPLPV NKINKGFTLV KMGFPTRIFE DTPTFCSCHS KLVYGGYFCP NCHSKVCSLP TVCPCCDLML ILSTHLARSY HHLMPLKTFA EVPTTEKFRS EDCFSCQSRF PILKNHKNGK LLTSSRYRCE DCKQEFCVDC DVFIHEILHN CPGCESKPVI T

Macromolecule #31: DNA repair helicase RAD25

• Macromolecule: Name: DNA repair helicase RAD25 / type: protein_or_peptide / ID: 31 / Enantiomer: LEVO
• Source (natural): Organism: yeast (fungus)

Sequence

String:

MTDVEGYQPK SKGKIFPDMG ESFFSSDEDS PATDAEIDEN YDDNRETSEG RGERDTGAMV TGLKKPRKKT KSSRHTAADS SMNQMDAKDK ALLQDTNSDI PADFVPDSVS GMFRSHDFSY LRLRPDHASR PLWISPSDGR IILESFSPLA EQAQDFLVTI AEPISRPSHI HEYKITAYSL YAAVSVGLET DDIISVLDRL SKVPVAESII NFIKGATISY GKVKLVIKHN RYFVETTQAD ILQMLLNDSV IGPLRIDSDH QVQPPEDVLQ QQLQQTAGKP ATNVNPNDVE AVFSAVIGGD NEREEEDDDI DAVHSFEIAN ESVEVVKKRC QEIDYPVLEE YDFRNDHRNP DLDIDLKPST QIRPYQEKSL SKMFGNGRAR SGIIVLPCGA GKTLVGITAA CTIKKSVIVL CTSSVSVMQW RQQFLQWCTL QPENCAVFTS DNKEMFQTES GLVVSTYSMV ANTRNRSHDS QKVMDFLTGR EWGFIILDEV HVVPAAMFRR VVSTIAAHAK LGLTATLVRE DDKIGDLNFL IGPKLYEANW MELSQKGHIA NVQCAEVWCP MTAEFYQEYL RETARKRMLL YIMNPTKFQA CQFLIQYHER RGDKIIVFSD NVYALQEYAL KMGKPFIYGS TPQQERMNIL QNFQYNDQIN TIFLSKVGDT SIDLPEATCL IQISSHYGSR RQEAQRLGRI LRAKRRNDEG FNAFFYSLVS KDTQEMYYST KRQAFLVDQG YAFKVITHLH GMENIPNLAY ASPRERRELL QEVLLKNEEA AGIEVGDDAD NSVGRGSNGH KRFKSKAVRG EGSLSGLAGG EDMAYMEYST NKNKELKEHH PLIRKMYYKN LKK

Macromolecule #32: Mediator of RNA polymerase II transcription subunit 1

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 1 / type: protein_or_peptide / ID: 32 / Enantiomer: LEVO
• Source (natural): Organism: yeast (fungus)

Sequence

String:

MVEGDSYVET LDSMIELFKD YKPGSITLEN ITRLCQTLGL ESFTEELSNE LSRLSTASKI IVIDVDYNKK QDRIQDVKLV LASNFDNFDY FNQRDGEHEK SNILLNSLTK YPDLKAFHNN LKFLYLLDAY SHIESDSTSH NNGSSDKSLD SSNASFNNQG KLDLFKYFTE LSHYIRQCFQ DNCCDFKVRT NLNDKFGIYI LTQGINGKEV PLAKIYLEEN KSDSQYRFYE YIYSQETKSW INESAENFSN GISLVMEIVA NAKESNYTDL IWFPEDFISP ELIIDKVTCS SNSSSSPPII DLFSNNNYNS RIQLMNDFTT KLINIKKFDI SNDNLDLISE ILKWVQWSRI VLQNVFKLVS TPSSNSNSSE LEPDYQAPFS TSTKDKNSST SNTEPIPRSN RHGSVVEASR RRRSSTNKSK RPSITEAMML KEEGLQQFNL HEILSEPAIE EENGDSIKEH STTMDGANDL GFTASVSNQE NAGTDIVMED HGVLQGTSQN YGTATADDAD IEMKDVSSKP SKPESSVLQL IVSEDHIILD TISECNLYDD VKCWSKFIEK FQDIVS

Macromolecule #33: Mediator of RNA polymerase II transcription subunit 2

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 2 / type: protein_or_peptide / ID: 33 / Enantiomer: LEVO
• Source (natural): Organism: yeast (fungus)

Sequence

String:

MVVQNSPVSS VHTANFSERG SNTRTMTYKN KLTVCFDDIL KVGAEMMMQQ QLKNVQLDSY LVNGFSQSQQ KLLKEKVKLF HGILDDLETS LSQSSSYLET LTALGKEKEK EREEAEKKRA EQENMRKVRE QEELKKRQEL EEASQQQQLQ QNSKEKNGLG LNFSTTAPAN TTDANGSKEN YQELGSLQSS SQTQLENANA ANNGAAFSPL TTTRIQSQQA QPSDVMFNDL NSMDISMFSG LDSTGFDSTA FNATVDETKG FDDNDSGNNY NDINISSIEN NINNNINSTK NGKDNNNESN KNNNGDEKNK NNNEDNENNN NSSEKNNNNN NNNNNNNDDN GNNNNNNSGN DNNNTTNNDS NNKNNSITTG NDNENIVNND LPTTVVSNPG DNPPPADNGE EYLTLNDFND LNIDWSTTGD NGELDLSGFN I

Macromolecule #34: Mediator of RNA polymerase II transcription subunit 3

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 3 / type: protein_or_peptide / ID: 34 / Enantiomer: LEVO
• Source (natural): Organism: yeast (fungus)

Sequence

String:

MDSIIPAGVK LDDLQVILAK NENETRDKVC KQINEARDEI LPLRLQFNEF IQIMANIDQE GSKQADRMAK YLHIRDKILQ LNDRFQTLSS HLEALQPLFS TVPEYLKTAD NRDRSFQLLE PLSTYNKNGN AVCSTATVVS TNHSAAASTP TTTATPHANP ITHAHSLSNP NSTATMQHNP LAGKRGPKSG STMGTPTVHN STAAAPIAAP KKPRKPRQTK KAKAQAQAQA QAQAQVYAQQ STVQTPITAS MAAALPNPTP SMINSVSPTN VMGTPLTNMM SPMGNAYSMG AQNQGGQVSM SQFNGSGNGS NPNTNTNSNN TPLQSQLNLN NLTPANILNM SMNNDFQQQQ QQQQQQQQPQ PQYNMNMGMN NMNNGGKELD SLDLNNLELG GLNMDFL

Macromolecule #35: Mediator of RNA polymerase II transcription subunit 4

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 4 / type: protein_or_peptide / ID: 35 / Enantiomer: LEVO
• Source (natural): Organism: yeast (fungus)

Sequence

String:

MSVQDTKAVE FSMGHIRSSS VSLVAEATSN TNSEDKLSKV QLYEDLCRYE DTLSKLVESV DRFKPNLDIA KDLIRTDEAL FENVKLLAEY DNIYRNLQKI DKDSEELDSK TRKILEILNE CHDELKALPM LEQVEFEKNT ILQQRSKINS TELLDYATKL SKFTKIPPTF DKGAVGPNNF IWPAEDALRR GMLAMASLHS KELTRIPGEE VEETEVPTVP PSQSEEQKGQ MAKKEGTPKT DSFIFDGTAK EVGDEADNTK DKEKEENNDD ALDLDLDLFD PDDF

Macromolecule #36: Mediator of RNA polymerase II transcription subunit 5

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 5 / type: protein_or_peptide / ID: 36 / Enantiomer: LEVO
• Source (natural): Organism: yeast (fungus)

Sequence

String:

MEKESVYNLA LKCAERQLTS MEFSNLYKEF FNEKFPSLIQ EEEEDTTTTA NINEVKKASD LVDTPSNNTA ATADTTHLHE ALDIVCSDFV KILNLEKPLI LADYIVEVLL VNYNSDMIKC FLPKLNSVRN SLLLAHFFSK SCSFFAKLSD TLIIDQVRKD LGNVIVPNIL SLDMNSMNKE LIAIVSKLLQ TTLKLSPSPI LLTSAGCKNG SFTLLNQLSQ TNKLLFKRVS QTFEAKLHFK DTKPFLNKDS TNEFVGSPSL TSPQYIPSPL SSTKPPGSVN SAAKYKDMKL LRYYKNIWLN NKIINWEISN PDFLSKYSAI TSSIFQESFN SVQNLDQLLT DLIETSFTCF AQFVSNKQYH QANSNLTLLE RKWVIFITKH LPLLILENSS RSPRVVTNAL DNIDEKVVKA IRIYFTEKDD NKTNNEDLFD DYPSTSLDIR HDFIKGLIML NLQPASVINN YLREDQMIDT SILPTRDDLF VRNLQGIQEV VHNTNSFIIS SLDTLELESI TESITHDSSN GLFQVLHNFE SVAPTKQREI VKAFLSIFED AIKELNYNRI AKICALLFFN FSHSLTTILS FSSPAALMKT LIKFVDLSRN GRNGSNGNDE SSEYETINIS LSFSWAILLI INLTQTYGIS VVDVALKYPE LSIKNSFIIN FISNLPNVSD KYYLEESNVN DSDMLTKSHN TVQSWLCDLF VNGSITDQLI QNIETRQLAN LIPFIVKQVL LSVEIGVLTD ISSLIGGFEY FLQPLLLVGL IKTFYWLEQF LSCVKNDTIS EDILQGIFNL LNTLFNPVTL NEDSKAFHTA VLRLNAIPLL KVLRKFRVQS QSNYGIYSSD AQGDPNLEPL IAKLVAVLNV SPVYDVDPRI INSENDYSRK QLGYGKFLIL NENPINKIMT NQINSFWSLH SSTYYNLDYL FELIELVTPK SFLFDVLKTL EYKLATYGVP GSENKRGSLD SEHVFDYFFY FLVLYDVKTA EEASQLIEYM ENDAKKSKGD VDIKGEDLHE KNDSAEVRQE TQPKAEATQD DDFDMLFGEN DTSTQAYEEE EENEDNDGNN RTNNVPMIKA EETPSKTNKI SILKRHSFAV LLHERKLLND LALENGEITK TENEKFISYH DKYLCMLKTC VF

Macromolecule #37: Mediator of RNA polymerase II transcription subunit 6

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 6 / type: protein_or_peptide / ID: 37 / Enantiomer: LEVO
• Source (natural): Organism: yeast (fungus)

Sequence

String:

MNVTPLDELQ WKSPEWIQVF GLRTENVLDY FAESPFFDKT SNNQVIKMQR QFSQLNDPNA AVNMTQNIMT LPDGKNGNLE EEFAYVDPAR RQILFKYPMY MQLEEELMKL DGTEYVLSSV REPDFWVIRK QRRTNNSGVG SAKGPEIIPL QDYYIIGANI YQSPTIFKIV QSRLMSTSYH LNSTLESLYD LIEFQPSQGV HYKVPTDTST TATAATNGNN AGGGSNKSSV RPTGGANMAT VPSTTNVNMT VNTMGTGGQT IDNGTGRTGN GNMGITTEML DKLMVTSIRS TPNYI

Macromolecule #38: Mediator of RNA polymerase II transcription subunit 7

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 7 / type: protein_or_peptide / ID: 38 / Enantiomer: LEVO
• Source (natural): Organism: yeast (fungus)

Sequence

String:

MSNDPGNEVS SLYPPPPPYV KFFTQSNLEK LPKYKEKKAA SAKQTAPNNS NGGSEEEITC ALDYLIPPPM PKNQQYRAFG SIWQVKDQLP DLESMGLTQL YKKSTENEST NYQYKIQELR KLLKSLLLNY LELIGVLSIN PDMYERKVEN IRTILVNIHH LLNEYRPHQS RESLIMLLEE QLEYKRGEIR EIEQVCKQVH DKLTSIQDTL RTGSQSPPSS SQ

Macromolecule #39: Mediator of RNA polymerase II transcription subunit 8

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 8 / type: protein_or_peptide / ID: 39 / Enantiomer: LEVO
• Source (natural): Organism: yeast (fungus)

Sequence

String:

MSQSTASLVP EGNQGSLQED VSFDFNGVPG QALDAVRMRL AQLTHSLRRI RDEMSKAELP QWYTLQSQLN VTLSQLVSVT STLQHFQETL DSTVVYPLPK FPTTSHESLV TTLLRKKNIP EVDEWMKYVR ETSGVTTALL KDEEIEKLLQ QDREITNWAR TTFRNEYGKH DFKNEESLSE EHASLLVRDS KPSKPFNVDD VLKFTFTGEK PIITGSTSTS SSN

Macromolecule #40: Mediator of RNA polymerase II transcription subunit 9

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 9 / type: protein_or_peptide / ID: 40 / Enantiomer: LEVO
• Source (natural): Organism: yeast (fungus)

Sequence

String:

MNLQNNVLNQ IHQILLPTNP TLDKPNAEAT KEEFSSAENR DEKDYLTNQQ PKNLSTPSTS SNGEFIPHIF YSLHQIRKDP NNLSNQLETL TGSIRHRLKL CKSLISENED TKDLLSKSPS EWQDIIHQRE QELQIKRDVL DDLYRKLQR

Macromolecule #41: Mediator of RNA polymerase II transcription subunit 10

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 10; type: protein_or_peptide / ID: 41 / Enantiomer: LEVO
• Source (natural): Organism: yeast (fungus)

Sequence

String:

MNGNSTNNEQ LQQELATTQD QVASIIESFV ELGVSIYDFP GTPEATKGMI TNLQRNVDRL YKLNVRSNDP QSSLSKVDIP LEVVQYIEDG RNPDIYTREF VEAIRRSNQY QRGKMHGLKQ LRDSLADKIV DEFPELKEPV EDIIKRTSPI DNVSNTH

Macromolecule #42: Mediator of RNA polymerase II transcription subunit 11

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 11; type: protein_or_peptide / ID: 42 / Enantiomer: LEVO
• Source (natural): Organism: yeast (fungus)

Sequence

String:

MQPPYIQERL KSLNDIETQL CSMLQEASQV TFIFGELKRG NESVKPQFEN HVKQFYERLD KSTTQLRKEI QLLDENVGTR LLPINVNKKA LGQDTEKMEE QLDLLSAILD PSKSK

Macromolecule #43: Mediator of RNA polymerase II transcription subunit 14

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 14; type: protein_or_peptide / ID: 43 / Enantiomer: LEVO
• Source (natural): Organism: yeast (fungus)

Sequence

String:

MTTTIGSPQM LANEERLSNE MHALKNRSEQ NGQEQQGPVK NTQLHGPSAT DPETTATQKE SLEMVPKDTS AATMTSAPPP ALPHVEINQV SLALVIRNLT VFTMKELAQY MKTNVHTQAN EPNSAKKIRF LQLIIFLRTQ FLKLYVLVKW TRTIKQNNFH VLIDLLNWFR TTNMNVNNCI WALKSSLNSM TNAKLPNVDL VTALEVLSLG RPNLPTHNFK LSGVSNSMDM VDGMAKVPIG LILQRLKDLN LTVSIKIALM NIPKPLNSYH IKNGRIYFTV PNEFEIQLST VNRQSPLFFV DLKLLFNTEA EQTVSAVTEA TSTNGDSENN EENSSSNGNN LPLNKPRLEK LINEILLKSN DPLLSLYNFL HKYVLTLQLY MVHREFLKLA NGGKFSKSNL IHNYDSKKST ITVRYWLNGK MDSKGKITIG IQRTTESLIL KWDNQSASRA KNMPVIYNNI VSNIEGILDE IMFNHARIIR SELLARDIFQ EDEENSDVLL FQLPTTCVSM APIQLKIDLL SGQFYFRNPT PLLSNYASKI NRAEGPEELA RILQQLKLDK IIHVLTTMFE NTGWSCSRII KIDKPIRTQV NTGGESVVKK EDNKYAIAGN STTNSDVSLL LQRDLFIRLP HWPLNWYLIL SIISSKTSCV VEKRIGKIVS QRGKWNLKYL DNSNVMTVKL ESITYQKIMI LQRTILNRII NHMLIDSLNQ LEIRNKICSS EMINEQKLPQ YIIQGSNTND NISIITLELE SFLEGSKALN SILESSMFLR IDYSNSQIRL YAKFKRNTMM IQCQIDKLYI HFVQEEPLAF YLEESFTNLG IIVQYLTKFR QKLMQLVVLT DVVERLHKNF ESENFKIIAL QPNEISFKYL SNNDEDDKDC TIKISTNDDS IKNLTVQLSP SNPQHIIQPF LDNSKMDYHF IFSYLQFTSS LFKALKVILN ERGGKFHESG SQYSTMVNIG LHNLNEYQIV YYNPQAGTKI TICIELKTVL HNGRDKIQFH IHFADVAHIT TKSPAYPMMH QVRNQVFMLD TKRLGTPESV KPANASHAIR LGNGVACDPS EIEPILMEIH NILKVDSNSS SS

Macromolecule #44: Mediator of RNA polymerase II transcription subunit 15

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 15; type: protein_or_peptide / ID: 44 / Enantiomer: LEVO
• Source (natural): Organism: yeast (fungus)

Sequence

String:

MSAAPVQDKD TLSNAERAKN VNGLLQVLMD INTLNGGSSD TADKIRIHAK NFEAALFAKS SSKKEYMDSM NEKVAVMRNT YNTRKNAVTA AAANNNIKPV EQHHINNLKN SGNSANNMNV NMNLNPQMFL NQQAQARQQV AQQLRNQQQQ QQQQQQQQRR QLTPQQQQLV NQMKVAPIPK QLLQRIPNIP PNINTWQQVT ALAQQKLLTP QDMEAAKEVY KIHQQLLFKA RLQQQQAQAQ AQANNNNNGL PQNGNINNNI NIPQQQQMQP PNSSANNNPL QQQSSQNTVP NVLNQINQIF SPEEQRSLLQ EAIETCKNFE KTQLGSTMTE PVKQSFIRKY INQKALRKIQ ALRDVKNNNN ANNNGSNLQR AQNVPMNIIQ QQQQQNTNNN DTIATSATPN AAAFSQQQNA SSKLYQMQQQ QQAQAQAQAQ AQAQAQAQAQ AQAAQAAQAQ AQAQAQAQAQ AQAQAQAQAQ AQAQAQAQAQ AHAQHQPSQQ PQQAQQQPNP LHGLTPTAKD VEVIKQLSLD ASKTNLRLTD VTNSLSNEEK EKIKMKLKQG QKLFVQVSNF APQVYIITKN ENFLKEVFQL RIFVKEILEK CAEGIFVVKL DTVDRLIIKY QKYWESMRIQ ILRRQAILRQ QQQMANNNGN PGTTSTGNNN NIATQQNMQQ SLQQMQHLQQ LKMQQQQQQQ QQQQQQQQQQ QQQQQQHIYP SSTPGVANYS AMANAPGNNI PYMNHKNTSS MDFLNSMENT PKVPVSAAAT PSLNKTINGK VNGRTKSNTI PVTSIPSTNK KLSISNAASQ QPTPRSASNT AKSTPNTNPS PLKTQTKNGT PNPNNMKTVQ SPMGAQPSYN SAIIENAFRK EELLLKDLEI RKLEISSRFK HRQEIFKDSP MDLFMSTLGD CLGIKDEEML TSCTIPKAVV DHINGSGKRK PTKAAQRARD QDSIDISIKD NKLVMKSKFN KSNRSYSIAL SNVAAIFKGI GGNFKDLSTL VHSSSPSTSS NMDVGNPRKR KASVLEISPQ DSIASVLSPD SNIMSDSKKI KVDSPDDPFM TKSGATTSEK QEVTNEAPFL TSGTSSEQFN VWDWNNWTSA T

Macromolecule #45: Mediator of RNA polymerase II transcription subunit 16

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 16; type: protein_or_peptide / ID: 45 / Enantiomer: LEVO
• Source (natural): Organism: yeast (fungus)

Sequence

String:

MMLGEHLMSW SKTGIIAYSD SQSSNANICL TFLESINGIN WRFHTPQKYV LHPQLHEVQY QESSSTLSTH STTTSVNGST TAGVGSTPNF GGNSNKSPPQ FFYNISSIHW NNWFSLPGDM LAVCDELGNM TMLITGQRPD RATTYEKLTM VFQDNVYKIY NHVMPLKPVD KLKPMNIERK QTRKEYNTSI LEFRWLTSSK SVIVSQFCAF DSSSNTYRSR AQQVPPYGVY HPPFIKYACL AIRKNGQIDF WYQFSNSKDH KKITLQLLDT SNQRFKDLQW LEFARITPMN DDQCMLITTY SKLSKNISFY KLHVNWNLNA TKPNVLNDPS LKIQFILSTT LDPTDDEGHV LKLENLHVVS KSSIEKDPSP EILVLYNVCD TSKSLVKRYR LAPTQLSAEY LVILKPDLNI DRNNSTNQIF QSRRYNLRRH SDIVLDKKVT LITSEMFDAF VSFYFEDGTI ESYNQNDWKL ETERLISQSQ LGKFKNIIAS PLSAGFNYGK LPLPPSVEWM KVSPSMCGVI VKQYNKKWPQ FYAAVQKNYA DPEKDSINAT ALAFGYVKSL HKQISAEDLT IAAKTHILRI SFLDRKRAKE FITTLLKSLY SFFNISPDAP KEIMDKIITS RPLQKIMLLQ LELGSCFSQE NIEEMARVIL YLKNVLFAFN GVARNFHFAI EQISNNSNQQ QNPKLFQTIF SKQDLIHSLI PVAKWFVKFI TYLTQEILIL INDPTNKEYT LVHGIFGAKM SRTLILSILN EIKKVTQIVA KFPETSYPIL NESSTFLKLV LSESPVDFEK FETFLVDVNN KFIALCEQQP SQEREFSLLV KAEIPPEYAK VGDFLLQYAN NAVISHANAA AVYFADTSGL KISNSEFFNP EIFHLLQPLE EGLIIDTDKL PIKNRTSKSF SKLLYDDVTC DKLSVSEISD GKLKRCSRCG SVTRAGNIIS SDKTIVPTSI QTKRWPTMYT RLCICSGMLF EMDG

Macromolecule #46: Mediator of RNA polymerase II transcription subunit 17

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 17; type: protein_or_peptide / ID: 46 / Enantiomer: LEVO
• Source (natural): Organism: yeast (fungus)

Sequence

String:

MTTEDPDSNH LSSETGIKLA LDPNLITLAL SSNPNSSLHS PTSDEPVPES AGKADTSIRL EGDELENKTK KDNDKNLKFL KNKDSLVSNP HEIYGSMPLE QLIPIILRQR GPGFKFVDLN EKELQNEIKQ LGSDSSDGHN SEKKDTDGAD ENVQIGEDFM EVDYEDKDNP VDSRNETDHK TNENGETDDN IETVMTQEQF VKRRRDMLEH INLAMNESSL ALEFVSLLLS SVKESTGMSS MSPFLRKVVK PSSLNSDKIP YVAPTKKEYI ELDILNKGWK LQSLNESKDL LRASFNKLSS ILQNEHDYWN KIMQSISNKD VIFKIRDRTS GQKLLAIKYG YEDSGSTYKH DRGIANIRNN IESQNLDLIP HSSSVFKGTD FVHSVKKFLR VRIFTKIESE DDYILSGESV MDRDSESEEA ETKDIRKQIQ LLKKIIFEKE LMYQIKKECA LLISYGVSIE NENKVIIELP NEKFEIELLS LDDDSIVNHE QDLPKINDKR ANLMLVMLRL LLVVIFKKTL RSRISSPHGL INLNVDDDIL IIRPILGKVR FANYKLLLKK IIKDYVLDIV PGSSITETEV EREQPQENKN IDDENITKLN KEIRAFDKLL NIPRRELKIN LPLTEHKSPN LSLMLESPNY CNALIHIKFS AGTEANAVSF DTTFSDFKEV EDFLHFIVAE YIQQKKV

Macromolecule #47: Mediator of RNA polymerase II transcription subunit 18

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 18; type: protein_or_peptide / ID: 47 / Enantiomer: LEVO
• Source (natural): Organism: yeast (fungus)

Sequence

String:

MVQQLSLFGS IGDDGYDLLI STLTTISGNP PLLYNSLCTV WKPNPSYDVE NVNSRNQLVE PNRIKLSKEV PFSYLIDETM MDKPLNFRIL KSFTNDKIPL NYAMTRNILH NTVPQVTNFN STNEDQNNSK HTEDTVNESR NSDDIIDVDM DASPAPSNES CSPWSLQISD IPAAGNNRSV SMQTIAETII LSSAGKNSSV SSLMNGLGYV FEFQYLTIGV KFFMKHGLIL ELQKIWQIEE AGNSQITSGG FLLKAYINVS RGTDIDRINY TETALMNLKK ELQGYIELSV PDRQSMDSRV AHGNILI

Macromolecule #48: Mediator of RNA polymerase II transcription subunit 19

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 19; type: protein_or_peptide / ID: 48 / Enantiomer: LEVO
• Source (natural): Organism: yeast (fungus)

Sequence

String:

MASRVDETTV PSYYYYVDPE TTYTYQQPNP LQDLISVYGL DDISRQVART NLDGTKAVKL RKSYKNQIAD LSGKFSTIPT RENGKGGQIA HILFQNNPDM MIQPPQQGQN MSEQQWREQL RNRDIALFQP PNFDWDLCSS VLSQFERSYP SEFANQNQGG AQAPFDIDDL AFDLDGTGKS QSGSNSGNNS KKRKNKSSGS SMATPTHSDS HEDMKRRRLE

Macromolecule #49: Mediator of RNA polymerase II transcription subunit 20

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 20; type: protein_or_peptide / ID: 49 / Enantiomer: LEVO
• Source (natural): Organism: yeast (fungus)

Sequence

String:

MGKSAVIFVE RATPATLTEL KDALSNSILS VRDPWSIDFR TYRCSIKNLP ADVSKLMYSI TFHHHGRQTV LIKDNSAMVT TAAAADIPPA LVFNGSSTGV PESIDTILSS KLSNIWMQRQ LIKGDAGETL ILDGLTVRLV NLFSSTGFKG LLIELQADEA GEFETKIAGI EGHLAEIRAK EYKTSSDSLG PDTSNEICDL AYQYVRALEL

Macromolecule #50: Mediator of RNA polymerase II transcription subunit 21

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 21; type: protein_or_peptide / ID: 50 / Enantiomer: LEVO
• Source (natural): Organism: yeast (fungus)

Sequence

String:

MTDRLTQLQI CLDQMTEQFC ATLNYIDKNH GFERLTVNEP QMSDKHATVV PPEEFSNTID ELSTDIILKT RQINKLIDSL PGVDVSAEEQ LRKIDMLQKK LVEVEDEKIE AIKKKEKLLR HVDSLIEDFV DGIANSKKST

Macromolecule #51: Mediator of RNA polymerase II transcription subunit 22

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 22; type: protein_or_peptide / ID: 51 / Enantiomer: LEVO
• Source (natural): Organism: yeast (fungus)

Sequence

String:

MSNQALYEKL EQTRTILSVK LAELINMTTI ADRNDDDEGS FAQENSELAV ATTSVMMVNN QTMQLIKNVQ DLLILTRSIK EKWLLNQIPV TEHSKVTRFD EKQIEELLDN CIETFVAEKT T

Macromolecule #52: Mediator of RNA polymerase II transcription subunit 31

• Macromolecule: Name: Mediator of RNA polymerase II transcription subunit 31; type: protein_or_peptide / ID: 52 / Enantiomer: LEVO
• Source (natural): Organism: yeast (fungus)

Sequence

String:

MSSTNGNAPA TPSSDQNPLP TRFEVELEFI QSLANIQYVT YLLTQQQIWK SPNFKNYLKY LEYWCNPPYS QCIVYPNCLF ILKLLNGFME SAIVNEDGLL EGLDELPKII QLQGPQWMNE MVERWAN

Macromolecule #53: 108 bp HIS4 promoter fragment

• Macromolecule: Name: 108 bp HIS4 promoter fragment / type: dna / ID: 53 / Classification: DNA
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)

Sequence

String:

ATATGACTCA TGAACAGTAG TATGCTGTGT ATATAATAGC TATGGAACGT TCGATTCACC TCCGATGTGT GTTGTACATA CATAAAAATA TCATAGCACA ACTGCGCT

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Concentration: 0.1 mg/mL

Buffer

pH: 7.5
Component:

Concentration	Formula	Name
80.0 mM	CH3CO2K	potassium acetate
20.0 mM	(HOCH2)3CNH2	Tris
2.0 mM	CH3CO2Mg	magnesium acetate
5.0 mM	C4H10O2S2	Dithiothreitol

Details: 80 mM potassium acetate, 20 mM Tris, pH 7.5, 2 mM magnesium acetate, 5 mM DTT

• Grid: Model: Quantifoil R2/1, R2/2 / Material: COPPER / Mesh: 200 / Support film - Material: CARBON / Support film - topology: HOLEY ARRAY / Pretreatment - Type: GLOW DISCHARGE
• Vitrification: Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 295 K / Instrument: FEI VITROBOT MARK IV
• Details: For cryo-EM, samples were chemically stabilized by fixation in glycerol gradients with increasing concentrations of glutaraldehyde (0-0.1%). Peak gradient fractions (~0.2 mg/mL) containing glutaraldhyde were quenched upon the addition of 50 mM Tris-HCl (pH 7.5) before flash-freezing and storage under liquid nitrogen.

Electron microscopy

• Microscope: FEI TECNAI F20
• Electron beam: Acceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
• Electron optics: C2 aperture diameter: 70.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.26 mm / Nominal defocus max: 2.5 µm / Nominal defocus min: 0.8 µm
• Sample stage: Specimen holder model: GATAN 626 SINGLE TILT LIQUID NITROGEN CRYO TRANSFER HOLDER; Cooling holder cryogen: NITROGEN
• Image recording: Film or detector model: GATAN ULTRASCAN 4000 (4k x 4k) / Average electron dose: 15.0 e/Ų
• Experimental equipment: Model: Tecnai F20 / Image courtesy: FEI Company

Image processing

• CTF correction: Software - Name: CTFFIND (ver. 3)
• Startup model: Type of model: OTHER; Details: Initial model generated using EMAN 2.1 e2initialmodel.py program using a small subset of 2D class averages generated from Relion 1.4 Class2D.
• Initial angle assignment: Type: RANDOM ASSIGNMENT / Software - Name: RELION (ver. 1.4)
• Final 3D classification: Software - Name: RELION (ver. 1.4)
• Final angle assignment: Type: OTHER / Software - Name: RELION (ver. 1.4) / Details: Bayesian Maximum Likelihood Approach
• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 21.9 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 1.4) / Number images used: 49900