EMDB-4093: Entire ovine respiratory complex I, Combined Map

Basic information

• Entry: Database: EMDB / ID: EMD-4093
• Title: Entire ovine respiratory complex I, Combined Map

Sample

• Complex: Mitochondrial complex IRespiratory complex I
  • Protein or peptide: x 44 types
• Ligand: x 10 types

Function / homology

Function:

Complex I biogenesis / Respiratory electron transport / mitochondrial respirasome / NADH dehydrogenase activity / respiratory chain complex I / oxidoreductase activity, acting on NAD(P)H / electron transport coupled proton transport / mitochondrial ATP synthesis coupled electron transport / NADH:ubiquinone reductase (H+-translocating) / mitochondrial respiratory chain complex I / apoptotic mitochondrial changes / mitochondrial respiratory chain complex I assembly / mitochondrial electron transport, NADH to ubiquinone / electron transport chain / NADH dehydrogenase (ubiquinone) activity / quinone binding / ATP synthesis coupled electron transport / respirasome / aerobic respiration / ATP metabolic process / reactive oxygen species metabolic process / respiratory electron transport chain / regulation of mitochondrial membrane potential / apoptotic signaling pathway / mitochondrial intermembrane space / 2 iron, 2 sulfur cluster binding / negative regulation of cell growth / circadian rhythm / fatty acid biosynthetic process / NAD binding / FMN binding / 4 iron, 4 sulfur cluster binding / mitochondrial inner membrane / response to oxidative stress / membrane => GO:0016020 / oxidoreductase activity / mitochondrial matrix / negative regulation of DNA-templated transcription / protein-containing complex binding / mitochondrion / nucleoplasm / metal ion binding / cytoplasm

Domain/homology:

Complex1_LYR-like / NADH-ubiquinone oxidoreductase 1 subunit C1 / NADH-ubiquinone oxidoreductase flavoprotein 3 / NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial / NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial / NADH:ubiquinone oxidoreductase, ESSS subunit / ESSS subunit of NADH:ubiquinone oxidoreductase (complex I) / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1, NDUB1 / MNLL subunit / NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial / NADH-ubiquinone oxidoreductase, subunit 10 / NADH-ubiquinone oxidoreductase subunit 10 / Soluble ligand binding domain / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex subunit 1 / NADH-ubiquinone oxidoreductase MWFE subunit / SLBB domain / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 / Zinc finger, CHCC-type / Zinc-finger domain / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, metazoa / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, animal type / NADH:ubiquinone oxidoreductase subunit B14.5a (Complex I-B14.5a) / NADH dehydrogenase 1 beta subcomplex subunit 2 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 / NADH dehydrogenase 1 alpha subcomplex subunit 3 / NADH dehydrogenase [ubiquinone] 1 subunit C2, NDUC2 / NADH:ubiquinone oxidoreductase, subunit NDUFB4 / NADH-ubiquinone oxidoreductase subunit b14.5b (NDUFC2) / NADH-ubiquinone oxidoreductase B15 subunit (NDUFB4) / NADH dehydrogenase 1, beta subcomplex, subunit 6 / NADH:ubiquinone oxidoreductase, NDUFB6/B17 subunit / NADH:ubiquinone oxidoreductase chain 4, N-terminal / NADH:ubiquinone oxidoreductase, chain 2 / NADH dehydrogenase subunit 2, C-terminal / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial / NADH:ubiquinone oxidoreductase, NDUFB5/SGDH subunit / NADH-ubiquinone oxidoreductase chain 4, amino terminus / NADH dehydrogenase subunit 2 C-terminus / NADH:ubiquinone oxidoreductase, NDUFB5/SGDH subunit / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 / GRIM-19 / NADH-ubiquinone oxidoreductase ASHI subunit (CI-ASHI or NDUFB8) / GRIM-19 protein / NADH dehydrogenase subunit 5, C-terminal / NADH dehydrogenase subunit 5 C-terminus / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 / NADH-ubiquinone oxidoreductase B12 subunit family / NDUFA6, LYR domain / NADH:ubiquinone oxidoreductase, NDUFS5-15kDa / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7, NDUB7 / NADH-ubiquinone oxidoreductase B18 subunit (NDUFB7) / NADH dehydrogenase ubiquinone Fe-S protein 4-like superfamily / NADH dehydrogenase ubiquinone Fe-S protein 4 / NADH dehydrogenase ubiquinone Fe-S protein 4, mitochondrial / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 2 / NDUFB9, LYR domain / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 / NADH ubiquinone oxidoreductase subunit NDUFA12 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 / NADH:ubiquinone oxidoreductase, iron-sulphur subunit 5 / ETC complex I subunit conserved region / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 6 / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 8 / NADH-quinone oxidoreductase, chain G, C-terminal / NADH-ubiquinone oxidoreductase subunit G, C-terminal / Deoxynucleoside kinase domain / Deoxynucleoside kinase / Respiratory-chain NADH dehydrogenase 20 Kd subunit signature. / NADH-ubiquinone oxidoreductase, 20 Kd subunit / NADH-quinone oxidoreductase, chain I / NAD(P)H-quinone oxidoreductase subunit D/H / NADH:ubiquinone oxidoreductase, 49kDa subunit, conserved site / Respiratory chain NADH dehydrogenase 49 Kd subunit signature. / NADH-quinone oxidoreductase, subunit D / Respiratory-chain NADH dehydrogenase, 49 Kd subunit / NADH:ubiquinone oxidoreductase, subunit G / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 3. / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 2. / NADH-ubiquinone/plastoquinone oxidoreductase chain 6, subunit NuoJ / NADH dehydrogenase, subunit C / NADH ubiquinone oxidoreductase, F subunit / Complex 1 LYR protein domain / NADH-plastoquinone oxidoreductase, chain 5 subgroup / NADH:ubiquinone oxidoreductase, 30kDa subunit, conserved site / Respiratory chain NADH dehydrogenase 30 Kd subunit signature. / NADH-quinone oxidoreductase, chain M/4 / Complex 1 protein (LYR family) / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 1. / NADH-ubiquinone oxidoreductase chain 4L/K / NADH:ubiquinone/plastoquinone oxidoreductase, chain 6 / NADH-ubiquinone/plastoquinone oxidoreductase chain 6 / NADH:ubiquinone oxidoreductase, 75kDa subunit, conserved site / NADH:ubiquinone oxidoreductase, 30kDa subunit / NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminal / NADH-quinone oxidoreductase, chain 5-like / NADH:ubiquinone oxidoreductase, 30kDa subunit superfamily / Respiratory-chain NADH dehydrogenase, 30 Kd subunit / NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminus

Component:

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 / NADH-ubiquinone oxidoreductase chain 1 / NADH-ubiquinone oxidoreductase chain 2 / NADH-ubiquinone oxidoreductase chain 3 / NADH-ubiquinone oxidoreductase chain 4L / NADH-ubiquinone oxidoreductase chain 4 / NADH-ubiquinone oxidoreductase chain 5 / NADH-ubiquinone oxidoreductase chain 6 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial / NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial / NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 / Acyl carrier protein / NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 / NADH dehydrogenase [ubiquinone] 1 subunit C2 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 / NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 / Complex I-PDSW / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 / NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial

• Biological species: Ovis aries (sheep) / Sheep (sheep)
• Method: single particle reconstruction / cryo EM / Resolution: 3.9 Å
• Authors: Fiedorczuk K / Letts JA / Sazanov LA

Funding support

United Kingdom, 1 items

Organization	Grant number	Country
Medical Research Council (United Kingdom)	MC_U105674180	United Kingdom

• Citation: Journal: Nature / Year: 2016; Title: Atomic structure of the entire mammalian mitochondrial complex I.; Authors: Karol Fiedorczuk / James A Letts / Gianluca Degliesposti / Karol Kaszuba / Mark Skehel / Leonid A Sazanov / ; Abstract: Mitochondrial complex I (also known as NADH:ubiquinone oxidoreductase) contributes to cellular energy production by transferring electrons from NADH to ubiquinone coupled to proton translocation across the membrane. It is the largest protein assembly of the respiratory chain with a total mass of 970 kilodaltons. Here we present a nearly complete atomic structure of ovine (Ovis aries) mitochondrial complex I at 3.9 Å resolution, solved by cryo-electron microscopy with cross-linking and mass-spectrometry mapping experiments. All 14 conserved core subunits and 31 mitochondria-specific supernumerary subunits are resolved within the L-shaped molecule. The hydrophilic matrix arm comprises flavin mononucleotide and 8 iron-sulfur clusters involved in electron transfer, and the membrane arm contains 78 transmembrane helices, mostly contributed by antiporter-like subunits involved in proton translocation. Supernumerary subunits form an interlinked, stabilizing shell around the conserved core. Tightly bound lipids (including cardiolipins) further stabilize interactions between the hydrophobic subunits. Subunits with possible regulatory roles contain additional cofactors, NADPH and two phosphopantetheine molecules, which are shown to be involved in inter-subunit interactions. We observe two different conformations of the complex, which may be related to the conformationally driven coupling mechanism and to the active-deactive transition of the enzyme. Our structure provides insight into the mechanism, assembly, maturation and dysfunction of mitochondrial complex I, and allows detailed molecular analysis of disease-causing mutations.

History

Deposition	Oct 28, 2016	-
Header (metadata) release	Nov 23, 2016	-
Map release	Nov 23, 2016	-
Update	Jun 2, 2021	-
Current status	Jun 2, 2021	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_4093.map.gz / Format: CCP4 / Size: 14.5 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 1.39 Å

Density

Contour Level	By AUTHOR: 0.15 / Movie #1: 0.15
Minimum - Maximum	-0.5494037 - 1.3139707
Average (Standard dev.)	0.0089633055 (±0.05093917)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order Z Y X Origin 0 0 0 Dimensions 141 209 129 Spacing 129 141 209
Cell	A: 179.31 Å / B: 195.99 Å / C: 290.51 Å α=β=γ: 90.0 °

CCP4 map header:

mode	Image stored as Reals
Å/pix. X/Y/Z	1.39	1.39	1.39
M x/y/z	129	141	209
origin x/y/z	0.000	0.000	0.000
length x/y/z	179.310	195.990	290.510
α/β/γ	90.000	90.000	90.000
start NX/NY/NZ	0	0	0
NX/NY/NZ	129	141	209
MAP C/R/S	3	2	1
start NC/NR/NS	0	0	0
NC/NR/NS	209	141	129
D min/max/mean	-0.549	1.314	0.009

Supplemental data

Sample components

Entire : Mitochondrial complex I

• Entire: Name: Mitochondrial complex IRespiratory complex I

Components

• Complex: Mitochondrial complex IRespiratory complex I
  • Protein or peptide: Mitochondrial complex I, 51 kDa subunit
  • Protein or peptide: Mitochondrial complex I, 24 kDa subunit
  • Protein or peptide: Mitochondrial complex I, 75 kDa subunit
  • Protein or peptide: Mitochondrial complex I, 49 kDa subunit
  • Protein or peptide: Mitochondrial complex I, 30 kDa subunit
  • Protein or peptide: Mitochondrial complex I, PSST subunit
  • Protein or peptide: Mitochondrial complex I, TYKY subunit
  • Protein or peptide: Mitochondrial complex I, ND1 subunit
  • Protein or peptide: Mitochondrial complex I, ND2 subunit
  • Protein or peptide: Mitochondrial complex I, ND3 subunit
  • Protein or peptide: Mitochondrial complex I, ND4 subunit
  • Protein or peptide: Mitochondrial complex I, ND4L subunit
  • Protein or peptide: Mitochondrial complex I, ND5 subunit
  • Protein or peptide: Mitochondrial complex I, ND6 subunit
  • Protein or peptide: Mitochondrial complex I, 10 kDa subunit
  • Protein or peptide: Mitochondrial complex I, 13 kDa subunit
  • Protein or peptide: Mitochondrial complex I, 18 kDa subunit
  • Protein or peptide: Mitochondrial complex I, 39 kDa subunit
  • Protein or peptide: Mitochondrial complex I, B8 subunit
  • Protein or peptide: Mitochondrial complex I, B13 subunit
  • Protein or peptide: Mitochondrial complex I, B14 subunit
  • Protein or peptide: Mitochondrial complex I, B14.5a subunit
  • Protein or peptide: Mitochondrial complex I, B17.2 subunit
  • Protein or peptide: Acyl carrier protein
  • Protein or peptide: Mitochondrial complex I, 42 kDa subunit
  • Protein or peptide: Mitochondrial complex I, 15 kDa subunit
  • Protein or peptide: Mitochondrial complex I, B9 subunit
  • Protein or peptide: Mitochondrial complex I, B12 subunit
  • Protein or peptide: Mitochondrial complex I, B14.5b subunit
  • Protein or peptide: Mitochondrial complex I, B15 subunit
  • Protein or peptide: Mitochondrial complex I, B16.6 subunit
  • Protein or peptide: Mitochondrial complex I, B17 subunit
  • Protein or peptide: Mitochondrial complex I, B18 subunit
  • Protein or peptide: Mitochondrial complex I, B22 subunit
  • Protein or peptide: Mitochondrial complex I, AGGG subunit
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial
  • Protein or peptide: Mitochondrial complex I, ESSS subunit
  • Protein or peptide: Mitochondrial complex I, KFYI subunit
  • Protein or peptide: Mitochondrial complex I, MNLL subunit
  • Protein or peptide: Mitochondrial complex I, MWFE subunit
  • Protein or peptide: Mitochondrial complex I, PDSW subunit
  • Protein or peptide: Mitochondrial complex I, PGIV subunit
  • Protein or peptide: Mitochondrial complex I, SGDH subunit
  • Protein or peptide: Mitochondrial complex I, B14.7 subunit
• Ligand: IRON/SULFUR CLUSTERIron–sulfur cluster
• Ligand: FLAVIN MONONUCLEOTIDE
• Ligand: FE2/S2 (INORGANIC) CLUSTER
• Ligand: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE
• Ligand: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE
• Ligand: CARDIOLIPIN
• Ligand: ZINC ION
• Ligand: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
• Ligand: S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate
• Ligand: 4'-PHOSPHOPANTETHEINEPhosphopantetheine

Supramolecule #1: Mitochondrial complex I

• Supramolecule: Name: Mitochondrial complex I / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#44
• Source (natural): Organism: Ovis aries (sheep)
• Molecular weight: Experimental: 970 KDa

Macromolecule #1: Mitochondrial complex I, 51 kDa subunit

• Macromolecule: Name: Mitochondrial complex I, 51 kDa subunit / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sheep (sheep)
• Molecular weight: Theoretical: 48.678281 KDa

Sequence

String:

FSGDTTAPKK TSFGSLKDED RIFTNLYGRH DWRLKGAQSR GDWYKTKEIL LKGPDWILGE VKTSGLRGRG GAGFPTGLKW SFMNKPSDG RPKYLVVNAD EGEPGTCKDR EIIRHDPHKL VEGCLVGGRA MGARAAYIYI RGEFYNEASN LQVAIREAYE A GLIGKNAC GSGYDFDVFV VRGAGAYICG EETALIESIE GKQGKPRLKP PFPADVGVFG CPTTVANVET VAVSPTICRR GG AWFASFG RERNSGTKLF NISGHVNNPC TVEEEMSVPL KELIEKHAGG VTGGWDNLLA VIPGGSSTPL IPKSVCETVL MDF DALIQA QTGLGTAAVI VMDRSTDIVK AIARLIEFYK HESCGQCTPC REGVDWMNKV MARFVRGDAR PAEIDSLWEI SKQI EGHTI CALGDGAAWP VQGLIRHFRP ELEERMQRFA QQHQARQAAS

Macromolecule #2: Mitochondrial complex I, 24 kDa subunit

• Macromolecule: Name: Mitochondrial complex I, 24 kDa subunit / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sheep (sheep)
• Molecular weight: Theoretical: 23.840428 KDa

Sequence

String:

GAGGALFVHR DTPKNNPETP FDFTPENYKR IEAIVKNYPE GHKAAAVLPV LDLAQRQNGW LPISAMNKVA EILQVPPMRV YEVATFYTM YNRKPVGKYH IQVCTTTPCM LRNSDSILEA IQKKLGIKVG ETTPDKLFTL IEVECLGACV NAPMVQINDN Y YEDLTPKD IEEIIDELKA GKIPKPGPRS GRFSCEPAGG LTSLTEPPKG PGFGVQAGL

Macromolecule #3: Mitochondrial complex I, 75 kDa subunit

• Macromolecule: Name: Mitochondrial complex I, 75 kDa subunit / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sheep (sheep)
• Molecular weight: Theoretical: 77.031156 KDa

Sequence

String:

TATAASNLIE VFVDGQSVMV EPGTTVLQAC EKVGMQIPRF CYHERLSVAG NCRMCLVEIE KAPKVVAACA MPVMKGWNIL TNSEKTKKA REGVMEFLLA NHPLDCPICD QGGECDLQDQ SMMFGSDRSR FLEGKRAVED KNIGPLVKTI MTRCIQCTRC I RFASEIAG VDDLGTTGRG NDMQVGTYIE KMFMSELSGN IIDICPVGAL TSKPYAFTAR PWETRKTESI DVMDAVGSNI VV STRTGEV MRILPRMHED INEEWISDKT RFAYDGLKRQ RLTEPMVRNE KGLLTHTTWE DALSRVAGML QSCQGNDVAA IAG GLVDAE ALIALKDLLN RVDSDTLCTE EVFPTAGAGT DLRSNYLLNT TIAGVEEADV VLLVGTNPRF EAPLFNARIR KSWL HNDLK VALIGSPVDL TYRYDHLGDS PKILQDIASG SHPFSQVLQE AKKPMVVLGS SALQRNDGAA ILAAVSNIAQ KIRTS SGVT GDWKVMNILH RIASQVAALD LGYKPGVEAI RKNPPKMLFL LGADGGCVTR QDLPKDCFIV YQGHHGDVGA PIADVI LPG AAYTEKSATY VNTEGRAQQT KVAVMPPGLA REDWKIIRAL SEIAGMTLPY DTLDQVRNRL EEVSPNLVRY DDVEGAN YF QQASELSKLV NQQLLADPLV PPQLTIKDFY MTDSISRASQ TMAKCVKAVT EGAHAVEEPS IC

Macromolecule #4: Mitochondrial complex I, 49 kDa subunit

• Macromolecule: Name: Mitochondrial complex I, 49 kDa subunit / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sheep (sheep)
• Molecular weight: Theoretical: 47.120078 KDa

Sequence

String:

MYPTKETAHW KPPPWNDVDP PKDTLVSNLT LNFGPQHPAA HGVLRLVMEL SGEMVRKCDP HIGLLHRGTE KLIEYKTYLQ ALPYFDRLD YVSMMCNEQA YSLAVEKLLN IQPPPRAQWI RVLFGEITRL LNHIMAVTTH ALDIGAMTPF FWMFEEREKM F EFYERVSG ARMHAAYVRP GGVHQDLPLG LMDDIYEFSK NFSLRIDELE EMLTNNRIWR NRTVDIGVVT AEDALNYGFS GV MLRGSGI QWDLRKTQPY DVYDQVEFDV PIGSRGDCYD RYLCRVEEMR QSIRIISQCL NKMPPGEIKV DDAKVSPPKR AEM KTSMES LIHHFKLYTE GYQVPPGATY TAIEAPKGEF GVYLVSDGSS RPYRCKIKAP GFAHLAGLDK MSKGHMLADV VAII GTQDI VFGEVDR

Macromolecule #5: Mitochondrial complex I, 30 kDa subunit

• Macromolecule: Name: Mitochondrial complex I, 30 kDa subunit / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sheep (sheep)
• Molecular weight: Theoretical: 26.441793 KDa

Sequence

String:

ESASADTRPT VRPRNDVAHK QLSAFGEYVA EILPKYVQQV QVSCFSELEI CIHPDGVIPV LTFLRDHSNA QFKSLADLTA VDIPTRQNR FEIVYNLLSL RFNSRIRVKT YTDELTPVES SVSVYKAANW YEREIWDMFG VFFANHPDLR RILTDYGFEG H PFRKDFPL SGYVELRYDD EVKRVVAEPV ELAQEFRKFD LNSPWEAFPA YRQPPESLKL EAGDKKPEAK

Macromolecule #6: Mitochondrial complex I, PSST subunit

• Macromolecule: Name: Mitochondrial complex I, PSST subunit / type: protein_or_peptide / ID: 6 / Details: NCBIProt XP_012033846.2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sheep (sheep)
• Molecular weight: Theoretical: 20.104531 KDa

Sequence

String:

PSSTQPAVSQ ARAVVPKPAA LPSSRGEYVV AKLDDLINWA RRSSLWPMTF GLACCAVEMM HMAAPRYDMD RFGVVFRASP RQSDVMIVA GTLTNKMAPA LRKVYDQMPE PRYVVSMGSC ANGGGYYHYS YSVVRGCDRI VPVDIYVPGC PPTAEALLYG I LQLQKKIK REKRLRIWYR R

Macromolecule #7: Mitochondrial complex I, TYKY subunit

• Macromolecule: Name: Mitochondrial complex I, TYKY subunit / type: protein_or_peptide / ID: 7 / Details: NCBIprot XP_011972879.1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sheep (sheep)
• Molecular weight: Theoretical: 20.219947 KDa

Sequence

String:

TYKYVNLREP SMDMKSVTDR AAQTLLWTEL IRGLGMTLSY LFREPATINY PFEKGPLSPR FRGEHALRRY PSGEERCIAC KLCEAVCPA QAITIEAEPR ADGSRRTTRY DIDMTKCIYC GFCQEACPVD AIVEGPNFEF STETHEELLY NKEKLLNNGD K WEAEIAAN IQADYLYR

Macromolecule #8: Mitochondrial complex I, ND1 subunit

• Macromolecule: Name: Mitochondrial complex I, ND1 subunit / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sheep (sheep)
• Molecular weight: Theoretical: 35.884902 KDa

Sequence

String:

MFMINVLTLI IPILLAVAFL TLVERKVLGY MQFRKGPNVV GPYGLLQPIA DAIKLFIKEP LRPATSSISM FILAPILALT LALTMWIPL PMPYPLINMN LGVLFMLAMS SLAVYSILWS GWASNSKYAL IGALRAVAQT ISYEVTLAII LLSVLLMNGS F TLSTLIIT QEQVWLIFPA WPLAMMWFIS TLAETNRAPF DLTEGESELV SGFNVEYAAG PFALFFMAEY ANIIMMNIFT TT LFLGAFH NPYMPELYTI NFTIKSLLLS ITFLWIRASY PRFRYDQLMH LLWKNFLPLT LALCMWHVSL PILLSSIPPQ T

Macromolecule #9: Mitochondrial complex I, ND2 subunit

• Macromolecule: Name: Mitochondrial complex I, ND2 subunit / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sheep (sheep)
• Molecular weight: Theoretical: 39.149805 KDa

Sequence

String:

MNPIILIIIL MTVMLGTIIV MISTHWLLIW IGFEMNMLAI IPIMMKKHNP RATEASTKYF LTQSTASMLL MMAIIINLMF SGQWTVMKL FNPMASMLMT MALAMKLGMA PFHFWVPEVT QGIPLSSGLI LLTWQKLAPM SVLYQILPSI NLDLILTLSI L SITIGGWG GLNQTQLRKI MAYSSIAHMG WMTAVLLYNP TMTLLNLIIY IIMTSTMFTL FMANSTTTTL SLSHTWNKAP IM TILVLIT LLSMGGLPPL SGFMPKWMII QEMTKNDSII LPTLMAITAL LNLYFYMRLT YSTALTMFPS TNNMKMKWQF PTT KRMTLL PTMTVLSTML LPLTPILSIL E

Macromolecule #10: Mitochondrial complex I, ND3 subunit

• Macromolecule: Name: Mitochondrial complex I, ND3 subunit / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sheep (sheep)
• Molecular weight: Theoretical: 13.10652 KDa

Sequence

String:

MNLMITLLTN FTLATLLVTI AFWLPQLNVY SEKTSPYECG FDPMGSARLP FSMKFFLVAI TFLLFDLEIA LLLPLPWASQ TTNLNTMLT MALLLIFLLA VSLAYEWTQK GLEWTE

Macromolecule #11: Mitochondrial complex I, ND4 subunit

• Macromolecule: Name: Mitochondrial complex I, ND4 subunit / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sheep (sheep)
• Molecular weight: Theoretical: 52.058734 KDa

Sequence

String:

MLKYIIPTMM LMPLTWLSKN SMIWINTTLH SLLISLTSLL LLNQFGDNSL NFSLTFFSDS LSTPLLILTM WLLPLMLMAS QHHLSKENL ARKKLFISML ILLQLFLIMT FTATELIFFY IMFEATLVPT LIIITRWGNQ TERLNAGLYF LFYTLAGSLP L LVALIYIQ NTMGSLNFLI LQYWVQPMPN SWSNTFMWLA CMMAFMVKMP LYGLHLWLPK AHVEAPIAGS MVLAAILLKL GG YGMMRIT LLLNPITDFM AYPFIMLSLW GMIMTSSICL RQTDLKSLIA YSSVSHMALV IVAILIQTPW SYMGATALMI AHG LTSSML FCLANSNYER VHSRTMILAR GLQTLLPLMA AWWLLASLTN LALPPSINLI GELFVVMSTF SWSNITIILM GLNM VITAL YSLYMLITTQ RGKHTHHINN ILPSFTRENA LMSLHMLPLL LLSLNPKIIL GPLY

Macromolecule #12: Mitochondrial complex I, ND4L subunit

• Macromolecule: Name: Mitochondrial complex I, ND4L subunit / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sheep (sheep)
• Molecular weight: Theoretical: 10.840228 KDa

Sequence

String:

MSLVYMNIMM AFTVSLTGLL MYRSHLMSSL LCLEGMMLSL FILATLMILN SHFTLASMMP IILLVFAACE AALGLSLLVM VSNTYGTDY VQNLNLLQC

Macromolecule #13: Mitochondrial complex I, ND5 subunit

• Macromolecule: Name: Mitochondrial complex I, ND5 subunit / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sheep (sheep)
• Molecular weight: Theoretical: 67.554945 KDa

Sequence

String:

MNLFSSLTLV TLILLTMPIA AINFNTHKFT NYPLYVKTTI SCAFITSMIP TMMFIHTGQE MIISNWHWLT IQTLKLSLSF KMDFFSMMF VPVALFVTWS IMEFSMWYMH SDPNINQFFK YLLLFLITML ILVTANNLFQ LFIGWEGVGI MSFLLIGWWY G RTDANTAA LQAILYNRIG DIGFILAMAW FLINLNTWDL QQIFMLNPND SNLPLMGLIL AATGKSAQFG LHPWLPSAME GP TPVSALL HSSTMVVAGI FLLIRFYPLT ENNKFGQSIM LCLGAMTTLF TAMCALTQND IKKIIAFSTS SQLGLMMVTI GIN QPHLAF LHICTHAFFK AMLFMCSGSI IHSLNDEQDI RKMGGLFKAM PFTTTALIIG SLALTGMPFL TGFYSKDLII ESAN TSYTN AWALLMTLVA TSFTAIYSTR IIFFALLGQP RFPTLININE NNPFLINSIK RLLIGSLFAG FIISNNIPPM TIPQM TMPH YLKMTALTVT ILGFILALEI SNTTHYLKFN YPSNTFKFSN LLGYYPTIMH RLTPYMNLTM SQKSASSLLD LIWLET ILP KTISLAQMKM STTITSQKGL IKLYFLSFLI TILIST

Macromolecule #14: Mitochondrial complex I, ND6 subunit

• Macromolecule: Name: Mitochondrial complex I, ND6 subunit / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sheep (sheep)
• Molecular weight: Theoretical: 19.126619 KDa

Sequence

String:

MMTYIVFILS IIFVMGFVGF SSKPSPIYGG LGLIVSGGVG CGIVLNFGGS FLGLMVFLIY LGGMMVVFGY TTAMATEQYP EVWVSNKVV LGTFITGLLM EFLMVYYVLK DKEVEIVFKF NGMGDWVIYD TGDSGFFSEE AMGIAALYSY GTWLVIVTGW S LLIGVVVI MEITRGN

Macromolecule #15: Mitochondrial complex I, 10 kDa subunit

• Macromolecule: Name: Mitochondrial complex I, 10 kDa subunit / type: protein_or_peptide / ID: 15 / Details: NCBIprot XP_011991231.1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sheep (sheep)
• Molecular weight: Theoretical: 8.423314 KDa

Sequence

String:

SAESGKNEKG LPPNPKKQSP PKKPASAAPT EPFDNTTYKN LQHHDYSTYT FLDLNLDLSK FRMPQPSSGR ESPRH

Macromolecule #16: Mitochondrial complex I, 13 kDa subunit

• Macromolecule: Name: Mitochondrial complex I, 13 kDa subunit / type: protein_or_peptide / ID: 16 / Details: NCBIprot XP_011980592.1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sheep (sheep)
• Molecular weight: Theoretical: 10.651796 KDa

Sequence

String:

GVRTSPTGEK VTHTGQVYDD EDYRRVRFVG RQKEVNENFA IDLIAEQPVS QVGSRVISCD GGGGALGHPR VYINLDKETK TGTCGYCGL QFRQQHH

Macromolecule #17: Mitochondrial complex I, 18 kDa subunit

• Macromolecule: Name: Mitochondrial complex I, 18 kDa subunit / type: protein_or_peptide / ID: 17 / Details: NCBIprot XP_004017046.1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sheep (sheep)
• Molecular weight: Theoretical: 15.3753 KDa

Sequence

String:

AQDQTRDTQL ITVDEKLDIT TLTGVPEEHI KTRKARIFVP ARNNMQSGVN NTKKWKMEFD TRERWENPLM GWASTADPLS NLVLTFSTK EDAIAFAEKN GWSYDVEERK VPKPKSKSYG ANFSWNKRTR VSTK

Macromolecule #18: Mitochondrial complex I, 39 kDa subunit

• Macromolecule: Name: Mitochondrial complex I, 39 kDa subunit / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sheep (sheep)
• Molecular weight: Theoretical: 38.660488 KDa

Sequence

String:

LHHAVIPHGK GGRSSVSGIV ATVFGATGFL GRYLVNHLGR MGSQVIVPYR CEPYDTMHLR PMGDLGQIIF MDWNGRDKDS IRRAVEHSN VVINLVGREW ETKNFDFEDV FVKIPQAIAQ VSKEAGVEKF IHISHLNADI KSSSKYLRNK AVGEKEVRET F PEATIIKP ADIFGREDRF LNYFANIRWF GGVPLISLGK KTVKQPVYIV DVTKGIINAI KDPDARGKTF AFVGPNRYLL FD LVQYVFA VAHRPFLPYP MPHFAYRWIG RLFEISPFEP WTTRDKVERI HTTDRTLPHL PGLEDLGVQA TPLELKAIEV LRR HRTYRW LSSEIEDVQP AK

Macromolecule #19: Mitochondrial complex I, B8 subunit

• Macromolecule: Name: Mitochondrial complex I, B8 subunit / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sheep (sheep)
• Molecular weight: Theoretical: 10.966627 KDa

Sequence

String:

AAAAAIRGVR GKLGLREIRI HLCQRSPGSQ GVRDFIEKRY VELKKANPDL PILIRECSDV QPKLWARYAF GQEKNVSLNN FSADQVTRA LENVLSSKA

Macromolecule #20: Mitochondrial complex I, B13 subunit

• Macromolecule: Name: Mitochondrial complex I, B13 subunit / type: protein_or_peptide / ID: 20 / Details: NCBIprot XP_004005022.1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sheep (sheep)
• Molecular weight: Theoretical: 13.156311 KDa

Sequence

String:

AGLLKKTTGL VGLAVCETPH ERLKILYTKI LDVLGHIPKN AAYRKYTEQI TNEKLSIVKA EPDVKKLEEQ LQGGQIEEVI LQAENELSL ARKMIQWKPW EPLVEEPPAS QWKWPI

Macromolecule #21: Mitochondrial complex I, B14 subunit

• Macromolecule: Name: Mitochondrial complex I, B14 subunit / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sheep (sheep)
• Molecular weight: Theoretical: 14.923245 KDa

Sequence

String:

AASGLRQAAV AASTSVKPIF SRDMNEAKRR VRELYRAWYR EVPNTVHLFQ LDISVKQGRD KVREMFKKNA HVTDPRVVDL LVIKGKMEL EETINVWKQR THVMRFFHET EAPRPKDFLS KFYVGHDP

Macromolecule #22: Mitochondrial complex I, B14.5a subunit

• Macromolecule: Name: Mitochondrial complex I, B14.5a subunit / type: protein_or_peptide / ID: 22 / Details: NCBIprot XP_004008614.1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sheep (sheep)
• Molecular weight: Theoretical: 12.399201 KDa

Sequence

String:

ASATRLIQGL RNWASGRDLQ AKLQLRYQEI SKRTQPPPKL PVGPSHRLSN NYYCARDGRR EAMPPSIVMS SQKVLVAGKP AESSAVAAS EKKAVSPAPP IKRWELSQDE PYL

Macromolecule #23: Mitochondrial complex I, B17.2 subunit

• Macromolecule: Name: Mitochondrial complex I, B17.2 subunit / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sheep (sheep)
• Molecular weight: Theoretical: 17.115508 KDa

Sequence

String:

MELLQVLKRG LQQVSGHGGL RGYLRVLFRA NDVRVGTLVG EDKYGNKYYE DNKQFFGRHR WVIYTTEMNG KNTFWDVDGS MVPPEWHRW LHCMTDDPPT VKPPTARKFI WTNHKFNLSG TPQQYVPYST TRKKIQEWVP PSTPYK

Macromolecule #24: Acyl carrier protein

• Macromolecule: Name: Acyl carrier protein / type: protein_or_peptide / ID: 24 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Sheep (sheep)
• Molecular weight: Theoretical: 10.119541 KDa

Sequence

String:

SDAPPLTLEG IKDRVLYVLK LYDKIDPEKL SVNSHFMKDL GLDSLDQVEI IMAMEDEFGF EIPDIDAEKL MCPQEIVDYI ADKKDVYE

Macromolecule #25: Mitochondrial complex I, 42 kDa subunit

• Macromolecule: Name: Mitochondrial complex I, 42 kDa subunit / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sheep (sheep)
• Molecular weight: Theoretical: 36.803762 KDa

Sequence

String:

LQYGPLAYIL GEKTTKKMTE NSKLITVDGN ICSGKSKLAK EVAEKLGLKH FPEAGIHYAD STTGDGKPLP VRFSGNCSLE KFYDDPKSN DGNSYRLQAW LYASRLLQYA DALEHLLSTG QGVVLERSIY SDFVFLEAMY RQGFIRKQCV DHYNQVKKVT V CEYLPPHV VIYVDVPVSE VQSRIQKKGN PHEMKITSAY LQDIENVYKG TFLPEMSEKC EVLQYSAWEA EDAEKVVEDI QY LKYDKGP WLDQDDRKLH NLRMLVQDKL EVLNYTSIPV FLPEVTIGAH QSDRVFQEFT ELPGRKYRAG YNEDVGDKWI WLK

Macromolecule #26: Mitochondrial complex I, 15 kDa subunit

• Macromolecule: Name: Mitochondrial complex I, 15 kDa subunit / type: protein_or_peptide / ID: 26 / Details: NCBIprot XP_004001873.1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sheep (sheep)
• Molecular weight: Theoretical: 12.474397 KDa

Sequence

String:

PFFDVQKKLG VDLDHWMTIQ SAEQPHRIPA RCHAFEKEWI ECAHGIGSIR AEKECKIEFE DFRECLLRQK TMKRLNAIKR QRDKLIKEG KYTPPPHHSG QEDLRP

Macromolecule #27: Mitochondrial complex I, B9 subunit

• Macromolecule: Name: Mitochondrial complex I, B9 subunit / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sheep (sheep)
• Molecular weight: Theoretical: 9.194655 KDa

Sequence

String:

APRVAAFLKN VWAKEPVLVA SFAIAGLAVI LPTLSPYTKY SLMINRATPY NYPVPLRDDG NMPDVPSHPQ DPQGPSLEWL KRL

Macromolecule #28: Mitochondrial complex I, B12 subunit

• Macromolecule: Name: Mitochondrial complex I, B12 subunit / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sheep (sheep)
• Molecular weight: Theoretical: 11.015482 KDa

Sequence

String:

AHGHGHEHGP SKMELPDYKQ WKIEGTPLET VQEKLAARGL RDPWGRNEAW RYMGGFANNV SFVGALLKGF KWGFAAFVVA VGAEYYLES QKKDKKHH

Macromolecule #29: Mitochondrial complex I, B14.5b subunit

• Macromolecule: Name: Mitochondrial complex I, B14.5b subunit / type: protein_or_peptide / ID: 29 / Details: NCBIprot XP_004019479.1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sheep (sheep)
• Molecular weight: Theoretical: 14.23142 KDa

Sequence

String:

MMTGRQARAP LQFLPDEARS LPPPKLTDPR LAYIGFLGYC SGLIDNAIRR RPVLSAGLHR QFLYITSFVF VGYYLLKRQD YMYAVRDHD MFSYIKSHPE DFPEKDKKTY REVFEEFHPV R

Macromolecule #30: Mitochondrial complex I, B15 subunit

• Macromolecule: Name: Mitochondrial complex I, B15 subunit / type: protein_or_peptide / ID: 30 / Details: NCBIprot XP_004003003.1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sheep (sheep)
• Molecular weight: Theoretical: 14.975999 KDa

Sequence

String:

SFPKYEPSRL ASLPTTLDPA EYDISSETRK AQAERLAIRS RLKREYQLQY NDPSRRGVVE DPALIRWTCA RSANVYPNFR PNTKTSLLG ALFGIGPLIF WYYVFKTDRD RKEKLIQEGK LDRTFNISY

Macromolecule #31: Mitochondrial complex I, B16.6 subunit

• Macromolecule: Name: Mitochondrial complex I, B16.6 subunit / type: protein_or_peptide / ID: 31 / Details: NCBIprot XP_004008450.1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sheep (sheep)
• Molecular weight: Theoretical: 16.635264 KDa

Sequence

String:

AASKVKQDMP PVGGYGPIDY KRNLPRRGLS GYSMFAVGIG ALLFGYWSMM RWNRERRRLQ IEDFEARIAL MPLLQAEKDR RVLQMLREN LEEEATIMKD VPGWKVGESV FHTTRWVTPM MGELYGLRTG EEILSSTYGF IWYT

Macromolecule #32: Mitochondrial complex I, B17 subunit

• Macromolecule: Name: Mitochondrial complex I, B17 subunit / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sheep (sheep)
• Molecular weight: Theoretical: 15.433929 KDa

Sequence

String:

SGYTPDEKLR LQQLRELRRR WLKDQELSPR EPVLPPRRVS PVERFWNKFL QDGALWKNVI YKTYRHSIFA FTHVLIPVWI IHYYLKYHV TTKPYTIVEK KPRIFPGDTI LETGEVIPPM KEFPDQHH

Macromolecule #33: Mitochondrial complex I, B18 subunit

• Macromolecule: Name: Mitochondrial complex I, B18 subunit / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sheep (sheep)
• Molecular weight: Theoretical: 16.28261 KDa

Sequence

String:

GAHLARRYLG DASVEPEPLR MPTFPPDYGF PERKEREMVA TQQEMNDAQL VLQQRDYCAH YLIRFLKCKR DSFPNFLACK HEQHDWDYC EHLDYVKRMK EFERERRLLQ RKKRREQREA DMAKGLGPGE VAPEVAL

Macromolecule #34: Mitochondrial complex I, B22 subunit

• Macromolecule: Name: Mitochondrial complex I, B22 subunit / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sheep (sheep)
• Molecular weight: Theoretical: 21.648715 KDa

Sequence

String:

AFLPSAAYLT HQQKVLQLYK RALRHLESRC VHRDKYRYFA CLLRARFDEH KNEKDMVKAT QLLREAEKEF WHGQHPQPYI FPESPGGTS YERYECYKVP EWCLDDWHPS EKAMYPDYFA KREQWKKLRR ESWEREVKQL QEETPVGGPR TEALPPARKQ G DLPPLWWH IVTRPRERPM

Macromolecule #35: Mitochondrial complex I, AGGG subunit

• Macromolecule: Name: Mitochondrial complex I, AGGG subunit / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sheep (sheep)
• Molecular weight: Theoretical: 8.500289 KDa

Sequence

String:

AGGGAHIEPR YRQFPQLTRS QVIQAEFFSA TMWFWILWRF WHDSDAVLGH FPYPDPSQWT DEELGIPPDD ED

Macromolecule #36: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mito...

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial; type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sheep (sheep)
• Molecular weight: Theoretical: 18.81807 KDa

Sequence

String:

ASHITKDMLP GPYPKTPEER AAAAKKYNMR VEDYEPYPDD GMGYGDYPKL PDRSQQERDP WYDWDHPDLR LNWGEPMHWD LDMYIRNRV DTSPTPVNWN LMCKHLFGFV AFMLFMFWVG ETYPTYQPVG PKQYPYNNLY LERGGDPNKE PEPVVHYEI

Macromolecule #37: Mitochondrial complex I, ESSS subunit

• Macromolecule: Name: Mitochondrial complex I, ESSS subunit / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sheep (sheep)
• Molecular weight: Theoretical: 14.455033 KDa

Sequence

String:

ESSSSRAVIA PSTLAGKRPS EPTLRWQEDP EPEDENLYEK NPDSHGYDKD PAVDVWNMRV VFFFGFSIVL VLGSTFVAYL PDYRMQEWA RREAERLVKY REAHGLPLME SNCFDPSKIQ LPEDED

Macromolecule #38: Mitochondrial complex I, KFYI subunit

• Macromolecule: Name: Mitochondrial complex I, KFYI subunit / type: protein_or_peptide / ID: 38 / Details: NCBIprot XP_004017292.1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sheep (sheep)
• Molecular weight: Theoretical: 5.808645 KDa

Sequence

String:

KFYIQEPPHG SPNWLKVGLT LGTSAFLWIY LIKQHNEDVL EYKRRNGLE

Macromolecule #39: Mitochondrial complex I, MNLL subunit

• Macromolecule: Name: Mitochondrial complex I, MNLL subunit / type: protein_or_peptide / ID: 39 / Details: NCBIprot XP_004018002.1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sheep (sheep)
• Molecular weight: Theoretical: 6.930083 KDa

Sequence

String:

MNLLQVVRDH WIHVLVPVGF VFGYYLDRKN DEKLAAFRNK SLLYKRELKP NEEVTWK

Macromolecule #40: Mitochondrial complex I, MWFE subunit

• Macromolecule: Name: Mitochondrial complex I, MWFE subunit / type: protein_or_peptide / ID: 40 / Details: NCBIprot NP_001305903.1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sheep (sheep)
• Molecular weight: Theoretical: 8.211519 KDa

Sequence

String:

MWFEVLPGIA VMGVCLFIPG MATARIHRFS NGGREKRVAH YSYQWYLMER DRRVSGVNRY YVSKGLENID

Macromolecule #41: Mitochondrial complex I, PDSW subunit

• Macromolecule: Name: Mitochondrial complex I, PDSW subunit / type: protein_or_peptide / ID: 41 / Details: NCBIprot XP_011999786.1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sheep (sheep)
• Molecular weight: Theoretical: 20.880752 KDa

Sequence

String:

PDSWDKDVYP EPPRRTPAPS PQTSLPNPIT YLTKAFDLLV DRPVTLVREF IERQHAKNKY YYYHREFRRV PDITECHEKD VLCMFEAEM QWKRDYKVDQ EIVNIIQERL KACQQREGES HRQNCAKELQ QFTQVVKAYQ DRYHDLGAHY SARKCLAKQK Q RMLAERKA TKEAAAA

Macromolecule #42: Mitochondrial complex I, PGIV subunit

• Macromolecule: Name: Mitochondrial complex I, PGIV subunit / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sheep (sheep)
• Molecular weight: Theoretical: 20.008014 KDa

Sequence

String:

PGIVELPSLE DLKVQEVKVS SSVLKAAAHH YGAQCDKPNK EFMLCRWEEK DPRRCLEEGK LVNQCALEFF RQIKRHCAEP FTEYWTCID YSGLQLFRRC RKEQAQFDKC VLDKLGWVRP DLGELSKVTK VKTDRPLPEN PYHSRARPEP NPEVEGDLKP A RHGSRLFF WTM

Macromolecule #43: Mitochondrial complex I, SGDH subunit

• Macromolecule: Name: Mitochondrial complex I, SGDH subunit / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sheep (sheep)
• Molecular weight: Theoretical: 16.714369 KDa

Sequence

String:

SGDHGKRLFI IKPSGFYDKR FLKLLRFYIL LTGIPVVIGI TLINVFIGEA ELAEIPEGYV PEHWEYFKHP ISRWIARTFF DAPEKNYER TMAILQIESE KAELRLKELE VRRLMRAKGD GPWFQYPTID KALIDHSPKA TPDN

Macromolecule #44: Mitochondrial complex I, B14.7 subunit

• Macromolecule: Name: Mitochondrial complex I, B14.7 subunit / type: protein_or_peptide / ID: 44 / Details: UniProt W5PAR2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sheep (sheep)
• Molecular weight: Theoretical: 10.145497 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #45: IRON/SULFUR CLUSTER

• Macromolecule: Name: IRON/SULFUR CLUSTER / type: ligand / ID: 45 / Number of copies: 6 / Formula: SF4
• Molecular weight: Theoretical: 351.64 Da

Chemical component information

ChemComp-FS1:
IRON/SULFUR CLUSTER / Iron–sulfur cluster

Macromolecule #46: FLAVIN MONONUCLEOTIDE

• Macromolecule: Name: FLAVIN MONONUCLEOTIDE / type: ligand / ID: 46 / Number of copies: 1 / Formula: FMN
• Molecular weight: Theoretical: 456.344 Da

Chemical component information

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE / Flavin mononucleotide

Macromolecule #47: FE2/S2 (INORGANIC) CLUSTER

• Macromolecule: Name: FE2/S2 (INORGANIC) CLUSTER / type: ligand / ID: 47 / Number of copies: 2 / Formula: FES
• Molecular weight: Theoretical: 175.82 Da

Chemical component information

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER / Iron–sulfur cluster

Macromolecule #48: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE

• Macromolecule: Name: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE / type: ligand / ID: 48 / Number of copies: 4 / Formula: 3PE
• Molecular weight: Theoretical: 748.065 Da

Macromolecule #49: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE

• Macromolecule: Name: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / type: ligand / ID: 49 / Number of copies: 4 / Formula: PC1
• Molecular weight: Theoretical: 790.145 Da

Chemical component information

ChemComp-PC1:
1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / phospholipid*YM / Phosphatidylcholine

Macromolecule #50: CARDIOLIPIN

• Macromolecule: Name: CARDIOLIPIN / type: ligand / ID: 50 / Number of copies: 4 / Formula: CDL
• Molecular weight: Theoretical: 1.464043 KDa

Chemical component information

ChemComp-CDL:
CARDIOLIPIN / phospholipid*YM / Cardiolipin

Macromolecule #51: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 51 / Number of copies: 1 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #52: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

• Macromolecule: Name: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE; type: ligand / ID: 52 / Number of copies: 1 / Formula: NDP
• Molecular weight: Theoretical: 745.421 Da

Chemical component information

ChemComp-NDP:
NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE / Nicotinamide adenine dinucleotide phosphate

Macromolecule #53: S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-b...

• Macromolecule: Name: S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate; type: ligand / ID: 53 / Number of copies: 1 / Formula: ZMP
• Molecular weight: Theoretical: 568.704 Da

Chemical component information

ChemComp-ZMP:
S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate

Macromolecule #54: 4'-PHOSPHOPANTETHEINE

• Macromolecule: Name: 4'-PHOSPHOPANTETHEINE / type: ligand / ID: 54 / Number of copies: 1 / Formula: PNS
• Molecular weight: Theoretical: 358.348 Da

Chemical component information

ChemComp-PNS:
4'-PHOSPHOPANTETHEINE / Phosphopantetheine

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Concentration: 5 mg/mL

Buffer

pH: 7.4
Component:

Concentration	Formula	Name
100.0 mM	NaClSodium chloride	Sodium Chloride
20.0 mM		HEPES
2.0 mM		EDTAEthylenediaminetetraacetic acid
1.5 %		Glycerol
0.2 %		Brij-35

• Grid: Model: Quantifoil R0.6/1 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Atmosphere: AIR
• Vitrification: Cryogen name: ETHANE / Chamber humidity: 90 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV / Details: 34s blotting.

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Calibrated magnification: 100720 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm / Nominal defocus max: 3.5 µm / Nominal defocus min: 0.5 µm / Nominal magnification: 59000
• Sample stage: Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
• Image recording: Film or detector model: FEI FALCON II (4k x 4k) / Detector mode: INTEGRATING / Digitization - Dimensions - Width: 4096 pixel / Digitization - Dimensions - Height: 4096 pixel / Digitization - Sampling interval: 14.0 µm / Digitization - Frames/image: 1-7 / Number grids imaged: 2 / Number real images: 2600 / Average exposure time: 1.0 sec. / Average electron dose: 26.0 e/Ų
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Particle selection: Number selected: 241000
• CTF correction: Software - Name: Gctf

Startup model

Type of model: EMDB MAP
EMDB ID:

2676

• Initial angle assignment: Type: NOT APPLICABLE
• Final 3D classification: Software - Name: RELION (ver. 1.4)
• Final angle assignment: Type: ANGULAR RECONSTITUTION / Software - Name: RELION (ver. 1.4)
• Final reconstruction: Number classes used: 1 / Resolution.type: BY AUTHOR / Resolution: 3.9 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 1.4) / Number images used: 82000

Atomic model buiding 1

Initial model

PDB ID:

4hea

Details: For the fourteen core subunits we used as a starting point our previous structure from T. thermophilus (PDB 4HEA) with sequence adjusted to ovine and side-chains rebuilt in SQWRL4. These are chains 1, 2, 3, 4, 5, 6, 9, H, A, J, K, N, M and L (with the same chain IDs as in 4HEA). For the remaining supernumerary subunits it was mostly de novo building with some help from homology modeling, which in most cases was not very useful due to low sequence homology. In few cases models produced by Phyre2 server were useful and were used as a starting point, due to the presence of templates with high coverage and confidence: 42kDa (chain k) - PDB ID 1P6X (xray) 39kDa (chain d) - PDB ID 3WJ7 (xray) SDAPs (chains j and X) - PDB ID 2DNW (NMR) B8 (chain e) - PDB ID 1S3A (NMR)

• Refinement: Space: REAL / Protocol: AB INITIO MODEL

Output model

PDB-5lnk:
Entire ovine respiratory complex I