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Yorodumi- PDB-4v49: Crystal Structure of a Streptomycin Dependent Ribosome from E. Co... -
+Open data
-Basic information
Entry | Database: PDB / ID: 4v49 | |||||||||
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Title | Crystal Structure of a Streptomycin Dependent Ribosome from E. Coli 70S Ribosome. | |||||||||
Components |
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Keywords | RIBOSOME / 30S RIBOSOMAL SUBUNIT / PROTEIN-RNA COMPLEX / PROTEIN-PROTEIN complex / RNA-RNA COMPLEX | |||||||||
Function / homology | RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) Function and homology information | |||||||||
Biological species | Escherichia coli (E. coli) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 8.7 Å | |||||||||
Authors | Vila-Sanjurjo, A. / Ridgeway, W.K. / Seymaner, V. / Zhang, W. / Santoso, S. / Yu, K. / Cate, J.H.D. | |||||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 2003 Title: X-ray Crystal Structures of the WT and a Hyper-Accurate Ribosome From Escherichia Coli Authors: Vila-Sanjurjo, A. / Ridgeway, W.K. / Seymaner, V. / Zhang, W. / Santoso, S. / Yu, K. / Cate, J.H.D. | |||||||||
History |
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Remark 400 | COMPOUND PDB ENTRIES 1PNS AND 1PNU REPRESENT ONE CRYSTAL STRUCTURE OF THE E. COLI 70S RIBOSOME. ...COMPOUND PDB ENTRIES 1PNS AND 1PNU REPRESENT ONE CRYSTAL STRUCTURE OF THE E. COLI 70S RIBOSOME. THIS FILE, 1PNS, CONTAINS THE 30S SUBUNIT, TWO TRNAS, AND ONE MRNA MOLECULE. THE 50S RIBOSOMAL SUBUNIT IS IN FILE 1PNU. | |||||||||
Remark 999 | SEQUENCE The 30S subunit is derived mainly from 1J5E, the T. thermophilus 30S subunit, with ...SEQUENCE The 30S subunit is derived mainly from 1J5E, the T. thermophilus 30S subunit, with modifications to the rRNA to make it match E. coli insertions and deletions. The sequence of this subunit represent that of pdb entry, 1J5E. As a result, no dbref was provided. |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4v49.cif.gz | 2.7 MB | Display | PDBx/mmCIF format |
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PDB format | pdb4v49.ent.gz | Display | PDB format | |
PDBx/mmJSON format | 4v49.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 4v49_validation.pdf.gz | 699.9 KB | Display | wwPDB validaton report |
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Full document | 4v49_full_validation.pdf.gz | 1.4 MB | Display | |
Data in XML | 4v49_validation.xml.gz | 204.5 KB | Display | |
Data in CIF | 4v49_validation.cif.gz | 341.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/v4/4v49 ftp://data.pdbj.org/pub/pdb/validation_reports/v4/4v49 | HTTPS FTP |
-Related structure data
Related structure data | 4v4aC 1j5eS 1lnr C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
-RNA chain , 5 types, 6 molecules AAAVAWAUB0B9
#1: RNA chain | Mass: 495740.656 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) | ||||||
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#2: RNA chain | Mass: 24518.570 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) #3: RNA chain | | Mass: 5726.460 Da / Num. of mol.: 1 / Source method: obtained synthetically #23: RNA chain | | Mass: 935779.625 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) #24: RNA chain | | Mass: 38029.777 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) |
-30S ribosomal protein ... , 19 types, 19 molecules ABACADAEAFAGAHAIAJAKALAMANAOAPAQARASAT
#4: Protein | Mass: 26987.271 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) |
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#5: Protein | Mass: 22862.430 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) |
#6: Protein | Mass: 24242.254 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) |
#7: Protein | Mass: 16331.079 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) |
#8: Protein | Mass: 11988.753 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) |
#9: Protein | Mass: 17919.775 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) |
#10: Protein | Mass: 15868.570 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) |
#11: Protein | Mass: 14298.466 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) |
#12: Protein | Mass: 11299.176 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) |
#13: Protein | Mass: 12606.369 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) |
#14: Protein | Mass: 13804.311 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) |
#15: Protein | Mass: 14207.666 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) |
#16: Protein | Mass: 7027.529 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) |
#17: Protein | Mass: 10447.213 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) |
#18: Protein | Mass: 9924.469 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) |
#19: Protein | Mass: 12193.475 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) |
#20: Protein | Mass: 8483.172 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) |
#21: Protein | Mass: 9203.743 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) |
#22: Protein | Mass: 10907.060 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) |
+50S ribosomal protein ... , 30 types, 30 molecules BABBBCBDBEBFBGBHBIBJBKBLBMBNBOBPBQBRBSBUBVBWBXBYBZB1B2B3B4B5
-Protein , 1 types, 1 molecules BT
#44: Protein | Mass: 18929.711 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 5.13 Å3/Da / Density % sol: 76 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 277 K / Method: vapor diffusion / pH: 6.5 Details: MPD, ethanol, magnesium chloride, ammonium chloride, spermine, spermidine, MES, pH 6.5, VAPOR DIFFUSION, temperature 277K | ||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions |
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-Data collection
Diffraction | Mean temperature: 110 K |
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Diffraction source | Source: SYNCHROTRON / Site: ALS / Beamline: 8.3.1 / Wavelength: 1.1 Å |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Aug 31, 2002 |
Radiation | Monochromator: Si 111 / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.1 Å / Relative weight: 1 |
Reflection | Resolution: 8.7→175 Å / Num. all: 37559 / Num. obs: 36338 / % possible obs: 98.5 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -3 / Redundancy: 5.2 % / Rsym value: 0.15 / Net I/σ(I): 9 |
Reflection shell | Resolution: 8.7→8.9 Å / Redundancy: 5.2 % / Mean I/σ(I) obs: 2.5 / Rsym value: 0.635 / % possible all: 99.2 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRIES 1J5E, 1LNR Resolution: 8.7→70 Å / Isotropic thermal model: ANISOTROPIC / Cross valid method: THROUGHOUT / σ(F): 0 Details: 89 RIGID BODIES REFINEMENT, GROUPED B-FACTOR REFINEMENT. There are many long O3'-P distances in this entry. Coordinates used for solving and refining this subunit mainly comes from PDB ...Details: 89 RIGID BODIES REFINEMENT, GROUPED B-FACTOR REFINEMENT. There are many long O3'-P distances in this entry. Coordinates used for solving and refining this subunit mainly comes from PDB entry, 1J5E, where the sequence represents that of T. thermophilus.
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Displacement parameters | Biso mean: 648.8 Å2 | |||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 8.7→70 Å
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