PDB-4a5q: Crystal structure of the chitinase Chi1 fitted into the...

Entry
Summary
Database / ID	PORTEIN DATA BANK (PDB) / 4a5q
Title	Crystal structure of the chitinase Chi1 fitted into the 3D structure of the Yersinia entomophaga toxin complex
Descriptor	CHI1 (E.C.3.2.1.14)
Keywords	HYDROLASE, BACTERIAL TOXIN, INSECTICIDE
Authors	Busby, J.N., Landsberg, M.J., Simpson, R.M., Jones, S.A., Hankamer, B., Hurst, M.R.H., Lott, J.S.
Date	Deposition: 2011-10-27, Release: 2011-11-16
PDBj Mine pages	Summary, Structural Details, Experimental Details, Functional Details
Other databases	RCSB-PDB, PDBe, CATH, CE, FSSP, SCOP, VAST
Structure Visualization
Movies	Movie Page #1: Depositted structure unit, Made by Jmol #2: Superimposing with simplified surface model of EM map, EMDB-1978, Made by Jmol #3: Superimposing with EM 3D map: EMDB-1978, Made by UCSF CHIMERA
Structure viewers	Yorodumi, jV4, Jmol, Biological unit (Images, jV)
Related Structure Data
Related Entries	EMDB-1978 Fit PDB-3oa5 Cite PDB-3ao5 Cite: data citing same article Fit: target map of fitting
Similar strucutres (beta)	Diagram PDB-3h3w List of similar structure data about Omokage system

Article
Citation - primary
Article	J. Mol. Biol., Vol. 415, Issue 2, Page 359-71, Year 2012
Title	Structural analysis of Chi1 Chitinase from Yen-Tc: the multisubunit insecticidal ABC toxin complex of Yersinia entomophaga.
Authors	Jason N Busby, Michael J Landsberg, Robert M Simpson, Sandra A Jones, Ben Hankamer, Mark R H Hurst, J Shaun Lott AgResearch Structural Biology Laboratory, School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand.
Keywords	Bacterial Toxins (chemistry), Chitin (metabolism, 1398-61-4), Chitinase (chemistry, 3.2.1.14), Cryoelectron Microscopy, Crystallography, X-Ray, Models, Molecular, New Zealand, Photorhabdus (genetics), Protein Structure, Quaternary, Soil Microbiology, Xenorhabdus (genetics), Yersinia (chemistry)
Links	PII: S0022-2836(11)01237-X, DOI: 10.1016/j.jmb.2011.11.018, PubMed: 22108167

Components
ID 1 : CHI1
Image
Description	CHI1
Type	polymer
Formula weight	60727.066 Da
Number of molecules	5
Ec	3.2.1.14
Source	Method: Isolated from a genetically manipulated source Gene: MH96\:\:9, ID:373547, YERSINIA SP. Host: ID:469008, ESCHERICHIA COLI , BL21(DE3), ROSETTA2, PDEST17, PLASMID
Links	UniProt: B6A876, Sequence view

Sample
Assembly
Aggregation state	PARTICLE
Details	PARTICLES WERE SELECTED USING SEMI-AUTOMATED PARTICLE SELECTION ( SWARMPS, E2BOXER.PY)
Name	INSECTICIDAL TC FROM YERSINIA ENTOMOPHAGA STRAIN MH96 (DELETION CONSTRUCT 9)(AKA YEN-TC K9)
Buffer
Name	25 MM TRIS, 130 MM NACL
Experiment
Reconstruction method	SINGLE PARTICLE
Sample preparation
pH	7.5
Sample concentration	0.1 mg/ml
Sample support
Details	OTHER

Processing
2D projection selection
Number of particles	10604
Software name	XMIPP, EMAN
3D reconstruction
Details	SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-1978.(DEPOSITION ID: 10324).
Method	CROSS-COMMON LINES, PROJECTION MATCHING
Resolution	17 A
3D fitting
Method	RIGID BODY
Temperature factor	22.09
Refinement Protocol	X-RAY
Refinement Space	REAL
Target criteria	CROSS-CORRELATION COEFFICIENT
3D fitting list
PDB entry ID	3OA5 PDB-3oa5
Refine
Ls d res high	17.00 A
ID	1
Refine hist
D res high	17.00
Total atoms	20005
Protein atoms	20005