PDB-3izj: Mm-cpn rls with ATP and AlFx

Entry
Summary
Database / ID	PORTEIN DATA BANK (PDB) / 3izj
Title	Mm-cpn rls with ATP and AlFx
Descriptor	Chaperonin
Keywords	CHAPERONE, Mm-cpn, maripaludis, chaperonin
PDBj Mine pages	Summary, Structural Details, Experimental Details, Functional Details
Other databases	RCSB-PDB, PDBe, CATH, CE, FSSP, SCOP, VAST
Structure Visualization
Movies	Movie Page #1: Depositted structure unit, Made by Jmol #2: Superimposing with EM 3D map: EMDB-5246, Made by UCSF CHIMERA
Structure viewers	Yorodumi, jV4, Jmol, Biological unit (Images, jV)
Related Structure Data
Related Entries	EMDB-5246 Fit PDB-3izh PDB-3izi PDB-3izk PDB-3izl PDB-3izm PDB-3izn Fit: target map of fitting
Similar strucutres (beta)	Diagram PDB-3j3x PDB-3izm PDB-3izi EMDB-5646 EMDB-5137 EMDB-5249 List of similar structure data about Omokage system

Article
Citation - primary
Article	Cell(Cambridge,Mass.), Vol. 144, Page: 240 - 252, Year: 2011,
Title	Dual Action of ATP Hydrolysis Couples Lid Closure to Substrate Release into the Group II Chaperonin Chamber.
Authors	Douglas, N.R., Reissmann, S., Zhang, J., Chen, B., Jakana, J., Kumar, R., Chiu, W., Frydman, J.
Links	DOI: 10.1016/j.cell.2010.12.017

Components
ID 1 : Chaperonin GroEL (Thermosome, HSP60 family)
Image
Description	Chaperonin
Type	polypeptide(L)
Formula weight	55119.980 Da
Number of molecules	16
ID	1
Source	Method: Isolated from a genetically manipulated source Gene: hsp60, MMP1515, ID:39152, Methanococcus maripaludis Host: ID:562, Escherichia coli
Links	UniProt: Q877G8, Sequence view

Sample
Assembly
Aggregation state	PARTICLE
Composition	16-mer
Experimental Mass	0.9
Theoretical Mass	0.9
Name	Mm-cpn rls with ATP and AlFx
Num components	2
Entity assembly
Assembly-ID	1
Name	Methanococcus maripaludis chaperonin
Type	PROTEIN
Experiment
Reconstruction method	SINGLE PARTICLE
Specimen type	VITREOUS ICE (CRYO EM)
Vitrification
Cryogen name	ETHANE
Humidity	100
Instrument	vitribot
Experiment
Method	ELECTRON MICROSCOPY

Electron Microscopy
Imaging
Microscope	Model: JEM3200FSC
Electron gun
Electron source	FIELD EMISSION GUN
Accelerating voltage	200 kV
Illumination mode	FLOOD BEAM
Lens
Mode	BRIGHT FIELD
Detector distance	0.0
Specimen holder
Specimen holder	Model: GATAN LIQUID NITROGEN, Type: Gatan side entry
Tilt angle	Min: 0.0 degrees, Max: 0.0 degrees
Detector
Type	Gatan 4Kx4K CCD camera