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Yorodumi- PDB-2mys: MYOSIN SUBFRAGMENT-1, ALPHA CARBON COORDINATES ONLY FOR THE TWO L... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2mys | |||||||||
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Title | MYOSIN SUBFRAGMENT-1, ALPHA CARBON COORDINATES ONLY FOR THE TWO LIGHT CHAINS | |||||||||
Components | (MYOSIN) x 3 | |||||||||
Keywords | MUSCLE PROTEIN / MYOSIN SUBFRAGMENT-1 / MYOSIN HEAD / MOTOR PROTEIN | |||||||||
Function / homology | Function and homology information regulation of myosin II filament assembly / contractile muscle fiber / Striated Muscle Contraction / myosin filament / myosin II complex / myosin complex / microfilament motor activity / myofibril / muscle contraction / skeletal muscle tissue development ...regulation of myosin II filament assembly / contractile muscle fiber / Striated Muscle Contraction / myosin filament / myosin II complex / myosin complex / microfilament motor activity / myofibril / muscle contraction / skeletal muscle tissue development / actin filament binding / calmodulin binding / calcium ion binding / ATP binding / cytoplasm Similarity search - Function | |||||||||
Biological species | Gallus gallus (chicken) | |||||||||
Method | X-RAY DIFFRACTION / Resolution: 2.8 Å | |||||||||
Authors | Rayment, I. / Holden, H.M. | |||||||||
Citation | Journal: Science / Year: 1993 Title: Three-dimensional structure of myosin subfragment-1: a molecular motor. Authors: Rayment, I. / Rypniewski, W.R. / Schmidt-Base, K. / Smith, R. / Tomchick, D.R. / Benning, M.M. / Winkelmann, D.A. / Wesenberg, G. / Holden, H.M. #1: Journal: Biochemistry / Year: 1995 Title: X-Ray Structures of the Myosin Motor Domain of Dictyostelium Discoideum Complexed with Mgadp.Befx and Mgadp.Alf4- Authors: Fisher, A.J. / Smith, C.A. / Thoden, J.B. / Smith, R. / Sutoh, K. / Holden, H.M. / Rayment, I. #2: Journal: Biochemistry / Year: 1995 Title: X-Ray Structure of the Magnesium(II)-Pyrophosphate Complex of the Truncated Head of Dictyostelium Discoideum Myosin to 2.7 A Resolution Authors: Smith, C.A. / Rayment, I. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2mys.cif.gz | 184.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2mys.ent.gz | 145.3 KB | Display | PDB format |
PDBx/mmJSON format | 2mys.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2mys_validation.pdf.gz | 424.6 KB | Display | wwPDB validaton report |
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Full document | 2mys_full_validation.pdf.gz | 502 KB | Display | |
Data in XML | 2mys_validation.xml.gz | 29 KB | Display | |
Data in CIF | 2mys_validation.cif.gz | 42.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/my/2mys ftp://data.pdbj.org/pub/pdb/validation_reports/my/2mys | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 96880.750 Da / Num. of mol.: 1 / Fragment: SUBFRAGMENT-1 / Source method: isolated from a natural source Details: PROTEOLYTIC FRAGMENT OF MYOSIN GENERATED BY PAPAIN DIGESTION Source: (natural) Gallus gallus (chicken) / Organ: SKELETAL / Tissue: PECTORALIS MUSCLE / References: UniProt: P13538 |
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#2: Protein | Mass: 18329.598 Da / Num. of mol.: 1 / Fragment: SUBFRAGMENT-1 / Source method: isolated from a natural source Details: PROTEOLYTIC FRAGMENT OF MYOSIN GENERATED BY PAPAIN DIGESTION Source: (natural) Gallus gallus (chicken) / Organ: SKELETAL / Tissue: PECTORALIS MUSCLE / References: UniProt: P02609 |
#3: Protein | Mass: 16340.213 Da / Num. of mol.: 1 / Fragment: SUBFRAGMENT-1 / Source method: isolated from a natural source Details: PROTEOLYTIC FRAGMENT OF MYOSIN GENERATED BY PAPAIN DIGESTION Source: (natural) Gallus gallus (chicken) / Organ: SKELETAL / Tissue: PECTORALIS MUSCLE / References: UniProt: P02605, UniProt: P02604*PLUS |
#4: Chemical | ChemComp-SO4 / |
#5: Chemical | ChemComp-MG / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 3.19 Å3/Da / Density % sol: 61.46 % | |||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS pH: 6.7 / Method: batch method / Details: macroseeding | |||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Wavelength: 1.5418 |
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Detector | Type: SIEMENS / Detector: AREA DETECTOR / Date: 1991 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Redundancy: 3.8 % / Rmerge(I) obs: 0.053 |
Reflection | *PLUS Highest resolution: 2.8 Å / Lowest resolution: 4.5 Å / Num. obs: 36781 / Num. measured all: 178986 / Rmerge(I) obs: 0.092 |
-Processing
Software |
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Refinement | Resolution: 2.8→30 Å / σ(F): 0 /
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Refinement step | Cycle: LAST / Resolution: 2.8→30 Å
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Software | *PLUS Name: TNT / Classification: refinement | |||||||||||||||
Refinement | *PLUS Rfactor all: 0.233 | |||||||||||||||
Solvent computation | *PLUS | |||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||
Refine LS restraints | *PLUS
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