+Open data
-Basic information
Entry | Database: PDB / ID: 1rss | ||||||
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Title | RIBOSOMAL PROTEIN S7 FROM THERMUS THERMOPHILUS | ||||||
Components | RIBOSOMAL PROTEIN S7 | ||||||
Keywords | RIBOSOMAL PROTEIN / RNA-BINDING / TRANSLATION | ||||||
Function / homology | Function and homology information ribosomal small subunit assembly / cytosolic small ribosomal subunit / tRNA binding / rRNA binding / structural constituent of ribosome / translation / mRNA binding Similarity search - Function | ||||||
Biological species | Thermus thermophilus (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MAD / Resolution: 1.9 Å | ||||||
Authors | Wimberly, B. / White, S. / Ramakrishnan, V. | ||||||
Citation | Journal: Structure / Year: 1997 Title: The structure of ribosomal protein S7 at 1.9 A resolution reveals a beta-hairpin motif that binds double-stranded nucleic acids. Authors: Wimberly, B.T. / White, S.W. / Ramakrishnan, V. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1rss.cif.gz | 42.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1rss.ent.gz | 29.9 KB | Display | PDB format |
PDBx/mmJSON format | 1rss.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1rss_validation.pdf.gz | 423.7 KB | Display | wwPDB validaton report |
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Full document | 1rss_full_validation.pdf.gz | 427.6 KB | Display | |
Data in XML | 1rss_validation.xml.gz | 9.4 KB | Display | |
Data in CIF | 1rss_validation.cif.gz | 12.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/rs/1rss ftp://data.pdbj.org/pub/pdb/validation_reports/rs/1rss | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 17351.115 Da / Num. of mol.: 1 / Mutation: 5 RESIDUES DELETED FROM N-TERMINUS Source method: isolated from a genetically manipulated source Source: (gene. exp.) Thermus thermophilus (bacteria) / Cell line: BL21 / Gene: S7 / Plasmid: PET-13A / Species (production host): Escherichia coli / Gene (production host): S7 / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 (DE3) / References: UniProt: P17291 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.7 Å3/Da / Density % sol: 40 % / Description: 2 WAVELENGTH MAD EXPERIMENT | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 277 K / pH: 8 Details: CRYSTALLIZED FROM 15% PEG 8000, 20% GLYCEROL, 6% MPD, 0.1 M NAHCO3, 0.1 M TRIS PH 8.0 AT 4 C, temperature 277K | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 4-13 ℃ / pH: 7.5 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K | |||||||||
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Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X12C / Wavelength: 0.98 / Wavelength: 0.98, 1.01 | |||||||||
Detector | Type: BRANDEIS / Detector: CCD / Date: May 15, 1997 | |||||||||
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||
Radiation wavelength |
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Reflection | Resolution: 1.9→100 Å / Num. obs: 13428 / % possible obs: 99.8 % / Observed criterion σ(I): 0 / Redundancy: 7.7 % / Biso Wilson estimate: 25.9 Å2 / Rmerge(I) obs: 0.033 / Rsym value: 0.04 / Net I/σ(I): 37 | |||||||||
Reflection shell | Resolution: 1.9→1.98 Å / Redundancy: 5 % / Rmerge(I) obs: 0.064 / Mean I/σ(I) obs: 5 / Rsym value: 0.307 / % possible all: 95 | |||||||||
Reflection | *PLUS Num. measured all: 103923 |
-Processing
Software |
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Refinement | Method to determine structure: MAD / Resolution: 1.9→6 Å / Rfactor Rfree error: 0.008 / Data cutoff high absF: 1000000 / Data cutoff low absF: 0.001 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0
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Displacement parameters | Biso mean: 35.9 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.9→6 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.9→2.01 Å / Rfactor Rfree error: 0.022 / Total num. of bins used: 6
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Xplor file |
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Software | *PLUS Name: X-PLOR / Version: 3.843 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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