Summary Database / ID EM DATA BANK (EMDB) / 5330 Authors Heuser T , Barber CF , Lin J , Krell J , Rebesco M , Porter ME , Nicastro D EMDB Sites Structure Visualization Movies X Movie Page #1 #2 #1 : Surface view with section colored by density value, Surface level: 127.5, Made by UCSF CHIMERA
#2 : Surface view colored by cylindrical radius, Surface level: 127.5, Made by UCSF CHIMERA
Supplemental images
Structure viewers (About PeppeR) , RCSB , PDBe )Related Structure Data Similar strucutres (beta)
Citation - Primary Article Proc. Natl. Acad. Sci. U.S.A. , Vol. 109, Issue 30, Page E2067-76, Year 2012Title Cryoelectron tomography reveals doublet-specific structures and unique interactions in the I1 dynein. Authors Thomas Heuser , Cynthia F Barber , Jianfeng Lin , Jeremy Krell , Matthew Rebesco , Mary E Porter , Daniela Nicastro Keywords Links
File Projections & Slices Size of images: Axes X SurfaceX X X X
Images are generated by Spider package .
Density
Contour Level: 127.5 (by author) , 127.5 (movie #1): Minimum - Maximum: 109.59519958 - 148.88063049 Average (Standard dev.): 124.20162964 (5.68234873)
Data Type Image stored as Reals Space Group Number 1 Map Geometry Axis Order : X Y Z Dimensions : 55 40 40 Origin : -21 -53 -64 Limit : 33 -14 -25 Spacing : 40 55 40
Unit Cell A = 380.0 A , B = 522.5 A , C = 380.0 A , 90.0 degrees , beta = 90.0 degrees , gamma = 90.0 degreesPixel Spacing X = 9.5 A , Y = 9.5 A , Z = 9.5 ACCP4 map header info Show mode Image stored as Reals A/pix X/Y/Z 9.5 9.5 9.5 M x/y/z 40 55 40 origin x/y/z 0.000 0.000 0.000 length x/y/z 380.000 522.500 380.000 alpha/beta/gamma 90.000 90.000 90.000 start NX/NY/NZ -62 -62 -62 NX/NY/NZ 125 125 125 MAP C/R/S 1 2 3 start NC/NR/NS -21 -53 -64 NC/NR/NS 55 40 40 start NC,NX/NR,NY/NS,NZ NC,NX/NR,NY/NS,NZ D min/max/mean 109.595 148.881 124.202
Annotation Details This is a subtomogram average of the I1 inner dynein complex in wild type Chlamydomonas flagella
Sample Preparation Specimen Conc 1 mg/ml Specimen Support Details Quantifoil holey carbon grids Cu 200 mesh R2/2 Buffer Details: 10 mM HEPES, pH 7.4, 25 mM NaCl, 4 mM MgSO4, 1 mM EGTA, 0.1 mM EDTA pH: 7.4 Vitrification Method front-side blotting for 2-3 seconds Cryogen Name ETHANE Instrument HOMEMADE PLUNGER Temperature 100 Kelvin Imaging Microscope FEI TECNAI F30 Date 20-FEB-2004 Electron Gun Electron Source FIELD EMISSION GUN Accelerating Voltage 300 kV Electron Dose 100 e/A**2 Illumination Mode OTHER Lens Magnification Nominal: 13500 X, Imaging Mode BRIGHT FIELD Defocus 6000 nm - 8000 nm Energy Filter Type: GATAN postcolumn filter GIF , Window: 0-20 eVSpecimen Holder Holder Eucentric ( GATAN LIQUID NITROGEN ) Tilt Angle -60 degrees - 60 degrees Temperature 80 Kelvin Camera Detector GATAN post-filter 2kx2k CCD Image Acquisition
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EMD-5330.map ( map file in CCP4 format, 354 KB )
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