+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-4034 | |||||||||
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Title | influenza virus membrane fusion | |||||||||
Map data | tomogram containing 3 contact zones | |||||||||
Sample |
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Biological species | influenza virus | |||||||||
Method | electron tomography / cryo EM | |||||||||
Authors | Calder LJ / Rosenthal PB | |||||||||
Citation | Journal: Nat Struct Mol Biol / Year: 2016 Title: Cryomicroscopy provides structural snapshots of influenza virus membrane fusion. Authors: Lesley J Calder / Peter B Rosenthal / Abstract: The lipid-enveloped influenza virus enters host cells during infection by binding cell-surface receptors and, after receptor-mediated endocytosis, fusing with the membrane of the endosome and ...The lipid-enveloped influenza virus enters host cells during infection by binding cell-surface receptors and, after receptor-mediated endocytosis, fusing with the membrane of the endosome and delivering the viral genome and transcription machinery into the host cell. These events are mediated by the hemagglutinin (HA) surface glycoprotein. At the low pH of the endosome, an irreversible conformational change in the HA, including the exposure of the hydrophobic fusion peptide, activates membrane fusion. Here we used electron cryomicroscopy and cryotomography to image the fusion of influenza virus with target membranes at low pH. We visualized structural intermediates of HA and their interactions with membranes during the course of membrane fusion as well as ultrastructural changes in the virus that accompany membrane fusion. Our observations are relevant to a wide range of protein-mediated membrane-fusion processes and demonstrate how dynamic membrane events may be studied by cryomicroscopy. | |||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_4034.map.gz | 90.2 MB | EMDB map data format | |
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Header (meta data) | emd-4034-v30.xml emd-4034.xml | 8.5 KB 8.5 KB | Display Display | EMDB header |
Images | emd_4034.png | 148.9 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-4034 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-4034 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_4034.map.gz / Format: CCP4 / Size: 283.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | tomogram containing 3 contact zones | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 4.3 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Sample components
-Entire : influenza virus
Entire | Name: influenza virus |
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Components |
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-Supramolecule #1: influenza virus
Supramolecule | Name: influenza virus / type: virus / ID: 1 / Parent: 0 / Macromolecule list: all / NCBI-ID: 11309 / Sci species name: influenza virus / Virus type: VIRION / Virus isolate: STRAIN / Virus enveloped: Yes / Virus empty: No |
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Host (natural) | Organism: Homo sapiens (human) |
-Macromolecule #1: influenza virus
Macromolecule | Name: influenza virus / type: other / ID: 1 / Classification: other |
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Sequence | String: XXX |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | electron tomography |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 5 Details: Sample was incubated at pH5 for 1min and then neutralised |
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Vitrification | Cryogen name: ETHANE |
Sectioning | Other: NO SECTIONING |
Fiducial marker | Manufacturer: British Biocell / Diameter: 10 nm |
-Electron microscopy
Microscope | FEI POLARA 300 |
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Electron beam | Acceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy |
Image recording | Film or detector model: TVIPS TEMCAM-F224 (2k x 2k) / Digitization - Dimensions - Width: 2048 pixel / Digitization - Dimensions - Height: 2048 pixel / Average electron dose: 2.0 e/Å2 |
Experimental equipment | Model: Tecnai Polara / Image courtesy: FEI Company |
-Image processing
Final reconstruction | Number images used: 40 |
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