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- EMDB-2630: Structural Studies on the Authentic Mumps Virus Nucleocapsid Show... -

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Basic information

Entry
Database: EMDB / ID: EMD-2630
TitleStructural Studies on the Authentic Mumps Virus Nucleocapsid Showing Uncoiling by the Phosphoprotein
Map dataMumps Nucleocapsid
Sample
  • Sample: Authentic mumps virus nucleocapsid
  • Virus: Mumps virus
Keywordsmumps / virus / nucleocapsid / phosphoprotein / replication
Function / homology
Function and homology information


negative stranded viral RNA transcription / negative stranded viral RNA replication / helical viral capsid / viral nucleocapsid / host cell cytoplasm / ribonucleoprotein complex / structural molecule activity / RNA binding
Similarity search - Function
Paramyxovirus nucleocapsid protein / Paramyxovirus nucleocapsid protein
Similarity search - Domain/homology
Biological speciesMumps virus
Methodhelical reconstruction / cryo EM / Resolution: 18.1 Å
AuthorsCox R / Pickar A / Qiu S / Tsao J / Rodenburg CM / Dokland T / Elson A / He B / Luo M
CitationJournal: Proc Natl Acad Sci U S A / Year: 2014
Title: Structural studies on the authentic mumps virus nucleocapsid showing uncoiling by the phosphoprotein.
Authors: Robert Cox / Adrian Pickar / Shihong Qiu / Jun Tsao / Cynthia Rodenburg / Terje Dokland / Andrew Elson / Biao He / Ming Luo /
Abstract: Mumps virus (MuV) is a highly contagious pathogen, and despite extensive vaccination campaigns, outbreaks continue to occur worldwide. The virus has a negative-sense, single-stranded RNA genome that ...Mumps virus (MuV) is a highly contagious pathogen, and despite extensive vaccination campaigns, outbreaks continue to occur worldwide. The virus has a negative-sense, single-stranded RNA genome that is encapsidated by the nucleocapsid protein (N) to form the nucleocapsid (NC). NC serves as the template for both transcription and replication. In this paper we solved an 18-Å-resolution structure of the authentic MuV NC using cryo-electron microscopy. We also observed the effects of phosphoprotein (P) binding on the MuV NC structure. The N-terminal domain of P (PNTD) has been shown to bind NC and appeared to induce uncoiling of the helical NC. Additionally, we solved a 25-Å-resolution structure of the authentic MuV NC bound with the C-terminal domain of P (PCTD). The location of the encapsidated viral genomic RNA was defined by modeling crystal structures of homologous negative strand RNA virus Ns in NC. Both the N-terminal and C-terminal domains of MuV P bind NC to participate in access to the genomic RNA by the viral RNA-dependent-RNA polymerase. These results provide critical insights on the structure-function of the MuV NC and the structural alterations that occur through its interactions with P.
History
DepositionApr 17, 2014-
Header (metadata) releaseApr 30, 2014-
Map releaseOct 15, 2014-
UpdateOct 29, 2014-
Current statusOct 29, 2014Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.00725
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by cylindrical radius
  • Surface level: 0.00725
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

EMD-2630.png

Downloads & links

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Map

FileDownload / File: emd_2630.map.gz / Format: CCP4 / Size: 29.8 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationMumps Nucleocapsid
Voxel sizeX=Y=Z: 2.29 Å
Density
Contour LevelBy AUTHOR: 0.00725 / Movie #1: 0.00725
Minimum - Maximum-0.00900029 - 0.01535856
Average (Standard dev.)0.00053587 (±0.00291266)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions200200200
Spacing200200200
CellA=B=C: 458.0 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z2.292.292.29
M x/y/z200200200
origin x/y/z0.0000.0000.000
length x/y/z458.000458.000458.000
α/β/γ90.00090.00090.000
start NX/NY/NZ-184-184-183
NX/NY/NZ368368368
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS200200200
D min/max/mean-0.0090.0150.001

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Supplemental data

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Sample components

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Entire : Authentic mumps virus nucleocapsid

EntireName: Authentic mumps virus nucleocapsid
Components
  • Sample: Authentic mumps virus nucleocapsid
  • Virus: Mumps virus

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Supramolecule #1000: Authentic mumps virus nucleocapsid

SupramoleculeName: Authentic mumps virus nucleocapsid / type: sample / ID: 1000 / Oligomeric state: helical / Number unique components: 1

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Supramolecule #1: Mumps virus

SupramoleculeName: Mumps virus / type: virus / ID: 1 / NCBI-ID: 11161 / Sci species name: Mumps virus / Sci species strain: Iowa / Virus type: VIRION / Virus isolate: STRAIN / Virus enveloped: Yes / Virus empty: No
Host (natural)Organism: Homo sapiens (human) / synonym: VERTEBRATES
Host systemOrganism: Chlorocebus aethiops (grivet) / Recombinant cell: vero

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Experimental details

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Structure determination

Methodcryo EM
Processinghelical reconstruction
Aggregation statehelical array

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Sample preparation

VitrificationCryogen name: ETHANE / Instrument: FEI VITROBOT MARK I

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Electron microscopy

MicroscopeFEI TECNAI F20
Electron beamAcceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: OTHER
Sample stageSpecimen holder model: GATAN LIQUID NITROGEN
DateApr 11, 2013
Image recordingCategory: CCD / Film or detector model: GATAN ULTRASCAN 4000 (4k x 4k)
Experimental equipment
Model: Tecnai F20 / Image courtesy: FEI Company

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Image processing

Final reconstructionApplied symmetry - Helical parameters - Δz: 5.32 Å
Applied symmetry - Helical parameters - Δ&Phi: 28.3 °
Applied symmetry - Helical parameters - Axial symmetry: C1 (asymmetric)
Resolution.type: BY AUTHOR / Resolution: 18.1 Å / Resolution method: OTHER / Software - Name: SPARX
DetailsIHRSR using SPARX/EMAN2 package

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