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Yorodumi- EMDB-1175: Structure of epsilon15 bacteriophage reveals genome organization ... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-1175 | |||||||||
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Title | Structure of epsilon15 bacteriophage reveals genome organization and DNA packaging/injection apparatus. | |||||||||
Map data | Density map of Epsilon15 phage | |||||||||
Sample |
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Biological species | Salmonella phage epsilon15 (virus) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 20.0 Å | |||||||||
Authors | Jiang W / Chang J / Jakana J / Weigele P / King J / Chiu W | |||||||||
Citation | Journal: Nature / Year: 2006 Title: Structure of epsilon15 bacteriophage reveals genome organization and DNA packaging/injection apparatus. Authors: Wen Jiang / Juan Chang / Joanita Jakana / Peter Weigele / Jonathan King / Wah Chiu / Abstract: The critical viral components for packaging DNA, recognizing and binding to host cells, and injecting the condensed DNA into the host are organized at a single vertex of many icosahedral viruses. ...The critical viral components for packaging DNA, recognizing and binding to host cells, and injecting the condensed DNA into the host are organized at a single vertex of many icosahedral viruses. These component structures do not share icosahedral symmetry and cannot be resolved using a conventional icosahedral averaging method. Here we report the structure of the entire infectious Salmonella bacteriophage epsilon15 (ref. 1) determined from single-particle cryo-electron microscopy, without icosahedral averaging. This structure displays not only the icosahedral shell of 60 hexamers and 11 pentamers, but also the non-icosahedral components at one pentameric vertex. The densities at this vertex can be identified as the 12-subunit portal complex sandwiched between an internal cylindrical core and an external tail hub connecting to six projecting trimeric tailspikes. The viral genome is packed as coaxial coils in at least three outer layers with approximately 90 terminal nucleotides extending through the protein core and the portal complex and poised for injection. The shell protein from icosahedral reconstruction at higher resolution exhibits a similar fold to that of other double-stranded DNA viruses including herpesvirus, suggesting a common ancestor among these diverse viruses. The image reconstruction approach should be applicable to studying other biological nanomachines with components of mixed symmetries. | |||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_1175.map.gz | 43.7 MB | EMDB map data format | |
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Header (meta data) | emd-1175-v30.xml emd-1175.xml | 11.1 KB 11.1 KB | Display Display | EMDB header |
Images | 1175.gif | 28 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-1175 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-1175 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_1175.map.gz / Format: CCP4 / Size: 89 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | Density map of Epsilon15 phage | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 4.08 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Sample components
-Entire : Bacteriophage Epsilon15
Entire | Name: Bacteriophage Epsilon15 |
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Components |
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-Supramolecule #1000: Bacteriophage Epsilon15
Supramolecule | Name: Bacteriophage Epsilon15 / type: sample / ID: 1000 Details: intact Epsilon15 phage particle with both icosahedral and non-icosahedral components Oligomeric state: icosahedral shell and12 fold portal and 6 fold tail and internal core and dsDNA genome Number unique components: 6 |
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Molecular weight | Theoretical: 500 KDa |
-Supramolecule #1: Salmonella phage epsilon15
Supramolecule | Name: Salmonella phage epsilon15 / type: virus / ID: 1 / NCBI-ID: 215158 / Sci species name: Salmonella phage epsilon15 / Virus type: VIRION / Virus isolate: STRAIN / Virus enveloped: No / Virus empty: No |
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Host (natural) | Organism: Salmonella (bacteria) / synonym: BACTERIA(EUBACTERIA) |
-Macromolecule #1: portal
Macromolecule | Name: portal / type: protein_or_peptide / ID: 1 / Number of copies: 12 / Oligomeric state: 12 / Recombinant expression: No |
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Source (natural) | Organism: Salmonella phage epsilon15 (virus) |
-Macromolecule #2: tailspike
Macromolecule | Name: tailspike / type: protein_or_peptide / ID: 2 / Number of copies: 18 / Oligomeric state: 3 / Recombinant expression: No |
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Source (natural) | Organism: Salmonella phage epsilon15 (virus) |
-Macromolecule #3: tail hub
Macromolecule | Name: tail hub / type: protein_or_peptide / ID: 3 / Recombinant expression: No |
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Source (natural) | Organism: Salmonella phage epsilon15 (virus) |
-Macromolecule #4: core
Macromolecule | Name: core / type: protein_or_peptide / ID: 4 / Recombinant expression: No |
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Source (natural) | Organism: Salmonella phage epsilon15 (virus) |
-Macromolecule #5: dsDNA genome
Macromolecule | Name: dsDNA genome / type: dna / ID: 5 / Classification: DNA / Structure: DOUBLE HELIX / Synthetic?: No |
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Source (natural) | Organism: Salmonella phage epsilon15 (virus) |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.5 / Details: 10 mM Tris pH7.5, 25 mM NaCl and 5 mM MgCl2 |
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Grid | Details: Quantifoil R2/2 grid |
Vitrification | Cryogen name: ETHANE / Instrument: OTHER / Details: Vitrification instrument: Vitrobot |
-Electron microscopy
Microscope | JEOL 2010F |
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Electron beam | Acceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2 mm / Nominal defocus max: 5.0 µm / Nominal defocus min: 1.0 µm / Nominal magnification: 40000 |
Sample stage | Specimen holder: side entry / Specimen holder model: GATAN LIQUID NITROGEN |
Temperature | Average: 100 K |
Details | imaged using JAMES software |
Date | Dec 18, 2004 |
Image recording | Category: CCD / Film or detector model: GENERIC GATAN (4k x 4k) / Digitization - Sampling interval: 15 µm / Number real images: 950 / Average electron dose: 10 e/Å2 / Details: Direct recording on Gatan 4k CCD / Bits/pixel: 12 |
Tilt angle min | 0 |
Tilt angle max | 0 |
-Image processing
CTF correction | Details: per particle |
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Final reconstruction | Applied symmetry - Point group: C1 (asymmetric) / Algorithm: OTHER / Resolution.type: BY AUTHOR / Resolution: 20.0 Å / Resolution method: FSC 0.5 CUT-OFF / Software - Name: SAVR / Number images used: 15000 |