 Entry |
| Summary |
| Database / ID | EM DATA BANK (EMDB) / 5205 |
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| Title | Structure of microtubule decorated with PRC1
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| Map | This is an image of section of microtubule decorated with PRC1 |
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| Sample | PRC1 |
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| Authors | Subramanian R, Wilson-Kubalek EM, Arthur CP, Bick MJ, Campbell EA, Darst SA, Milligan RA, Kapoor TM |
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| Date | Deposition: 2010-06-30, Header release: 2010-09-10, Map release: 2010-09-10, Last update: 2010-10-19 |
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| EMDB Sites |  EMDB @PDBe (EU), EMDB @RCSB (USA) |
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| Structure Visualization |
| Movies |  Movie Page
#1: Surface view with section colored by density value, Surface level: 14, Made by UCSF CHIMERA #2: Surface view colored by cylindrical radius, Surface level: 14, Made by UCSF CHIMERA |
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| Supplemental images |
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| Structure viewers |  Yorodumi,  Launch PeppeR (About PeppeR), Volume viewer (RCSB, PDBe) |
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| Related Structure Data |
| Related Entries |
Cite: data citing same article |
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| Similar strucutres (beta) |
 List of similar structure data  about Omokage system |
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 Article |
| Citation - Primary |
| Article | Cell, Vol. 142, Issue 3, Page 433-43, Year 2010 |
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| Title | Insights into antiparallel microtubule crosslinking by PRC1, a conserved nonmotor microtubule binding protein. |
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| Authors | Radhika Subramanian, Elizabeth M Wilson-Kubalek, Christopher P Arthur, Matthew J Bick, Elizabeth A Campbell, Seth A Darst, Ronald A Milligan, Tarun M Kapoor
Laboratory of Chemistry and Cell Biology, The Rockefeller University, New York, NY 10065, USA. |
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| Keywords |  Cell Cycle Proteins (chemistry), Cryoelectron Microscopy, Humans, Microtubules (metabolism), Models, Molecular, PRC1 protein, human, Protein Structure, Tertiary, Spectrin (metabolism, 12634-43-4) |
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| Links | PII: S0092-8674(10)00781-6, DOI: 10.1016/j.cell.2010.07.012, PubMed: 20691902, PMC: PMC2966277 |
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 Map |
| File |  EMD-5205.map ( map file in CCP4 format, 12049 KB ) |
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| Projections & Slices | Size of images: | Axes |  Z (Sec.) |  Y (Row.) |  X (Col.) |
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| Surface |  |  |  |
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Projections |  |  |  |
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Slices (1/3) |  |  |  |
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Slices (1/2) |  |  |  |
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Slices (2/3) |  |  |  |
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Images are generated by Spider package. |
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| Density |
| Contour Level: | 2.5 (by author), 14 (movie #1): |
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| Minimum - Maximum: | -32 - 50 |
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| Average (Standard dev.): | 0.847337 (10.7397) |
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| Data Type | Image stored as Reals |
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| Space Group Number | 1 |
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| Map Geometry | | Axis Order : | X | Y | Z |
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| Dimensions : | 171 | 171 | 103 |
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| Origin : | 0 | 0 | 0 |
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| Limit : | 170 | 170 | 102 |
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| Spacing : | 171 | 171 | 103 |
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| Unit Cell | A = 913.995 A , B = 913.995 A , C = 550.535 A ,
alpha = 90 degrees , beta = 90 degrees , gamma = 90 degrees |
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| Pixel Spacing | | X | Y | Z |
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| EMDB info. | 5.345 | 5.345 | 5.345 |
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| CCP4 map header | 5.345 | 5.345 | 5.345 |
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| EM Navigator Movie #1 | 3.47 | 3.47 | 3.47 |
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| CCP4 map header info | | mode | Image stored as Reals |
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| A/pix X/Y/Z | 5.345 | 5.345 | 5.345 |
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| M x/y/z | 171 | 171 | 103 |
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| origin x/y/z | 0.000 | 0.000 | 0.000 |
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| length x/y/z | 913.995 | 913.995 | 550.535 |
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| alpha/beta/gamma | 90.000 | 90.000 | 90.000 |
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| start NX/NY/NZ | -99 | -99 | -99 |
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| NX/NY/NZ | 200 | 200 | 200 |
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| MAP C/R/S | 1 | 2 | 3 |
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| start NC/NR/NS | 0 | 0 | 0 |
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| NC/NR/NS | 171 | 171 | 103 |
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| start NC,NX/NR,NY/NS,NZ | | | |
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| NC,NX/NR,NY/NS,NZ | | | |
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| D min/max/mean | -32.000 | 50.000 | 0.847 |
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| Annotation Details | This is an image of section of microtubule decorated with PRC1 |
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