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Structure of microtubule decorated with PRC1

by helical reconstruction, at 18 A resolution

Movie

Orientation:

#1: Surface view with section colored by density value, Surface level: 14, Image by UCSF CHIMERA

#2: Surface view colored by cylindrical radius, Surface level: 14, Image by UCSF CHIMERA

Entry
Summary
Database / IDEM DATA BANK (EMDB) / 5205
TitleStructure of microtubule decorated with PRC1
MapThis is an image of section of microtubule decorated with PRC1
SamplePRC1
AuthorsSubramanian R, Wilson-Kubalek EM, Arthur CP, Bick MJ, Campbell EA, Darst SA, Milligan RA, Kapoor TM
DateDeposition: 2010-06-30, Header release: 2010-09-10, Map release: 2010-09-10, Last update: 2010-10-19
EMDB SitesEMDB @PDBe (EU), EMDB @RCSB (USA)
Structure Visualization
MoviesMovie Page

#1: Surface view with section colored by density value, Surface level: 14, Image by UCSF CHIMERA

#2: Surface view colored by cylindrical radius, Surface level: 14, Image by UCSF CHIMERA

Supplemental images
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Article
Citation - Primary
ArticleCell, Vol. 142, Issue 3, Page 433-43, Year 2010
TitleInsights into antiparallel microtubule crosslinking by PRC1, a conserved nonmotor microtubule binding protein.
AuthorsRadhika Subramanian, Elizabeth M Wilson-Kubalek, Christopher P Arthur, Matthew J Bick, Elizabeth A Campbell, Seth A Darst, Ronald A Milligan, Tarun M Kapoor
KeywordsCell Cycle Proteins (chemistry), Cryoelectron Microscopy, Humans, Microtubules (metabolism), Models, Molecular, PRC1 protein, human, Protein Structure, Tertiary, Spectrin (metabolism, 12634-43-4)
LinksPII: S0092-8674(10)00781-6, DOI: 10.1016/j.cell.2010.07.012, PubMed: 20691902, PMC: PMC2966277
Map
Fileemd_5205.map.gz ( map file in CCP4 format, 11766 KB )
Projections & SlicesSize of images:
AxesZ (Sec.)Y (Row.)X (Col.)
103 pix
5.345 A/pix
= 550.535 A
171 pix
5.345 A/pix
= 913.995 A
171 pix
5.345 A/pix
= 913.995 A

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider package.

Density
Contour Level:2.5 (by author), 14 (movie #1):
Minimum - Maximum: -32 - 50
Average (Standard dev.): 0.847337 (10.7397)
Data TypeImage stored as Reals
Space Group Number1
Map Geometry
Axis orderXYZ
Dimensions171171103
Origin000
Limit170170102
Spacing171171103
Unit CellA: 913.995 A, B: 913.995 A, C: 550.535 A
Alpha=beta=gamma: 90 degrees
Pixel Spacing
XYZ
EMDB info.5.3455.3455.345
CCP4 map header5.3455.3455.345
EM Navigator Movie #13.473.473.47
CCP4 map header info
modeImage stored as Reals
A/pix X/Y/Z5.3455.3455.345
M x/y/z171171103
origin x/y/z0.0000.0000.000
length x/y/z913.995913.995550.535
alpha/beta/gamma90.00090.00090.000
start NX/NY/NZ-99-99-99
NX/NY/NZ200200200
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS171171103
start NC,NX/NR,NY/NS,NZ
NC,NX/NR,NY/NS,NZ
D min/max/mean-32.00050.0000.847
Annotation DetailsThis is an image of section of microtubule decorated with PRC1
Supplement
Images
Images
Sample
NamePRC1
Number of Components2
DetailsDecorated microtubules
Component #1: protein - PRC1
Scientific nameMicrotubule
Common NamePRC1
Scientific Name of Speciesunidentified
NCBI taxonomy32644
Recombinant expressionYes
Experiment
Sample Preparation
Helical ParametersHand: LEFT HANDED
Specimen Statefilament
Vitrification
Cryogen NameETHANE
InstrumentNONE
Imaging
MicroscopeFEI TECNAI F20
Date02-FEB-2010
Electron Gun
Electron SourceFIELD EMISSION GUN
Accelerating Voltage120 kV
Electron Dose10 e/A**2
Illumination ModeFLOOD BEAM
Lens
MagnificationNominal: 35000
Nominal Cs2 mm
Imaging ModeBRIGHT FIELD
Defocus900 nm - 2000 nm
Specimen Holder
HolderEucentric
ModelGATAN LIQUID NITROGEN
Tilt Angle0 degrees - 0 degrees
Temperature93 K ( 93 - 93 K)
Camera
DetectorGENERIC GATAN
Image Acquisition
#1
Number of Digital Images10
Processing
Methodhelical reconstruction
3D reconstruction
AlgorithmFourier Bessel inversion
SoftwarePHOELIX
CTF CorrectionCTF correction of each micrograph
Resolution By Author18 A
Resolution MethodCoherent phase determination
Download
Data from EMDB
Header (meta data in XML format)emd-5205.xml (6.3 KB)
Map dataemd_5205.map.gz (1.6 MB)
Imagesemd_5205_1.png (296.1 KB)
FTP directoryftp://ftp.pdbj.org/pub/emdb/structures/EMD-5205
Movie files
movie #1
.mp4 (H.264/MPEG-4 AVC format), 3.3 MB
.webm (WebM/VP8 format), 5.2 MB
Session file for UCSF-Chimera, 26.1 KB
movie #2
.mp4 (H.264/MPEG-4 AVC format), 3.1 MB
.webm (WebM/VP8 format), 4.6 MB
Session file for UCSF-Chimera, 26.2 KB