Mammalian mitochondrial translation initiation factor 2
by single particle reconstruction, at 10.8 A resolution

#1: Depositted structure unit, Made by Jmol
#2: Superimposing with EM 3D map: EMDB-1855, Made by UCSF CHIMERA
Entry | |
| Summary | |
| Database / ID | PORTEIN DATA BANK (PDB) / 3izy |
|---|---|
| Title | Mammalian mitochondrial translation initiation factor 2 |
| Descriptor | PROTEIN/RNA Complex |
| Keywords | RNA, RIBOSOMAL PROTEIN, translation initiation factor 2, IF2, E coli |
| Authors | Yassin, A.S., Haque, E, Datta, P.P, Elmore, K, Banavali, N.K., Spremulli, L.L., Agrawal, R.K. |
| Date | Deposition: 2011-01-19, Release: 2011-03-23 |
| PDBj Mine pages | Summary, Structural Details, Experimental Details, Functional Details |
| Other databases | RCSB-PDB, PDBe, CATH, CE, FSSP, SCOP, VAST |
| Structure Visualization | |
| Movies | Movie Page#1: Depositted structure unit, Made by Jmol #2: Superimposing with EM 3D map: EMDB-1855, Made by UCSF CHIMERA |
| Structure viewers | Yorodumi, jV4, Jmol, Biological unit (Images, jV) |
| Related Structure Data | |
| Related Entries |
Cite: data citing same article Fit: target map of fitting |
| Similar strucutres (beta) |
List of similar structure data about Omokage system |
Article | |
| Citation - primary | |
| Article | Proc. Natl. Acad. Sci. U.S.A., Vol. 108, Issue 10, Page 3918-23, Year 2011 |
|---|---|
| Title | Insertion domain within mammalian mitochondrial translation initiation factor 2 serves the role of eubacterial initiation factor 1. |
| Authors | Aymen S Yassin, Md Emdadul Haque, Partha P Datta, Kevin Elmore, Nilesh K Banavali, Linda L Spremulli, Rajendra K Agrawal Wadsworth Center, New York State Department of Health, Empire State Plaza, Albany, NY 12201-0509, USA. |
| Keywords | Amino Acids (chemistry), Animals, Cattle, Cryoelectron Microscopy, Eubacterium (metabolism), Eukaryotic Initiation Factor-1 (chemistry), Eukaryotic Initiation Factor-2 (chemistry), Models, Molecular, Oxidative Phosphorylation, Ribosomes (metabolism) |
| Links | DOI: 10.1073/pnas.1017425108, PubMed: 21368145, PMC: PMC3053986 |
Components | |
| ID 1 : tRNA-Phe | |
| Image | ![]() ![]() |
|---|---|
| Description | tRNA-Phe |
| Type | polymer |
| Formula weight | 24518.730 Da |
| Number of molecules | 1 |
| Source | Method: Isolated from a natural source Common name: cow NCBI taxonomy: ID:9913Organism scientific: Bos taurus |
| Links | Sequence view |
| ID 2 : IF-2(Mt), IF-2Mt, IF2(mt) | |
| Image | ![]() ![]() |
| Description | Translation initiation factor IF-2, mitochondrial |
| Type | polymer |
| Formula weight | 59602.836 Da |
| Number of molecules | 1 |
| Source | Method: Isolated from a natural source Common name: cow NCBI taxonomy: ID:9913Organism scientific: Bos taurus |
| Links | UniProt: P46198, Sequence view |
Sample | |
| Assembly | |
| Aggregation state | PARTICLE |
|---|---|
| Name | Mammalian mitochondrial translation initiation factor 2 interaction with tRNA |
| Experiment | |
| Reconstruction method | SINGLE PARTICLE |
| Specimen type | VITREOUS ICE (CRYO EM) |
Electron Microscopy | |
| Imaging | |
| Microscope | model: Tecnai F20 |
|---|---|
| Electron gun | |
| Electron source | FEG |
| Accelerating voltage | 200 kV |
| Lens | |
| Magnification | calibrated: 50760 X, nominal: 50000 X |
| Detector | |
| Type | Kodak S0163 |
Processing | |
| Single particle entity | |
| Symmetry type | ASYMMETRIC |
|---|---|
| 3D reconstruction | |
| Actual pixel size | 2.76 A/pix |
| CTF correction method | Every Micrograph |
| Method | Back Projection |
| Number of particles | 121742 |
| Resolution | 10.8 A |
| Software | Spider |
| Refine hist | |
| Total atoms | 5802 |
| Nucleic acid atoms | 1622 |
| Protein atoms | 4180 |
Download | |||
| PDB format | |||
| All | pdb3izy.ent.gz pdb3izy.ent (uncompressed file) | ||
|---|---|---|---|
| Header only | pdb3izy.ent.gz | ||
| mmCIF format | |||
| mmCIF | 3izy.cif.gz | ||
| XML format | |||
| All | 3izy.xml.gz | ||
| No-atom | 3izy-noatom.xml.gz | ||
| Ext-atom | 3izy-extatom.xml.gz | ||
| Movie files | |||
| movie #1 |
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| movie #2 |
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