+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-37381 | |||||||||
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Title | Structure of TaHKT2;1 in NaCl at 2.6 Angstroms resolution | |||||||||
Map data | ||||||||||
Sample |
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Keywords | HKT / salt tolerance / ion selectivity / TRANSPORT PROTEIN | |||||||||
Function / homology | Cation transporter / Cation transport protein / metal ion transport / monoatomic cation transmembrane transporter activity / plasma membrane / Uncharacterized protein Function and homology information | |||||||||
Biological species | Arabidopsis thaliana (thale cress) / Triticum aestivum (bread wheat) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.6 Å | |||||||||
Authors | Wang JQ | |||||||||
Funding support | China, 1 items
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Citation | Journal: To Be Published Title: Structure of TaHKT2;1 in NaCl at 2.6 Angstroms resolution Authors: Wang JQ | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_37381.map.gz | 33.1 MB | EMDB map data format | |
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Header (meta data) | emd-37381-v30.xml emd-37381.xml | 13.1 KB 13.1 KB | Display Display | EMDB header |
Images | emd_37381.png | 42 KB | ||
Filedesc metadata | emd-37381.cif.gz | 5.3 KB | ||
Others | emd_37381_half_map_1.map.gz emd_37381_half_map_2.map.gz | 31.2 MB 30.3 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-37381 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-37381 | HTTPS FTP |
-Related structure data
Related structure data | 8w9tMC M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_37381.map.gz / Format: CCP4 / Size: 35.3 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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Voxel size | X=Y=Z: 0.93 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #1
File | emd_37381_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_37381_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
-Entire : Structure of TaHKT2;1 in NaCl at 2.6 Angstroms resolution
Entire | Name: Structure of TaHKT2;1 in NaCl at 2.6 Angstroms resolution |
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Components |
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-Supramolecule #1: Structure of TaHKT2;1 in NaCl at 2.6 Angstroms resolution
Supramolecule | Name: Structure of TaHKT2;1 in NaCl at 2.6 Angstroms resolution type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: Arabidopsis thaliana (thale cress) |
-Macromolecule #1: HKT2
Macromolecule | Name: HKT2 / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO |
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Source (natural) | Organism: Triticum aestivum (bread wheat) |
Molecular weight | Theoretical: 60.20059 KDa |
Recombinant expression | Organism: Homo sapiens (human) |
Sequence | String: MHLFLTLVHS TMDRVKRFYQ DFIHIKLHSF SRISRYVVDS IVFIYRFVAL HVHPFWIQLS YFLAIAILGS VLLISLKPSN PEFSPPYID MLYLSTSALT VSGLSTVKME DLSSSQIVVL TLLMLVGGEI FVSLLGLMLR VNHQDMQDLP SVKISSVPVE L EVLDLANS ...String: MHLFLTLVHS TMDRVKRFYQ DFIHIKLHSF SRISRYVVDS IVFIYRFVAL HVHPFWIQLS YFLAIAILGS VLLISLKPSN PEFSPPYID MLYLSTSALT VSGLSTVKME DLSSSQIVVL TLLMLVGGEI FVSLLGLMLR VNHQDMQDLP SVKISSVPVE L EVLDLANS MALCDESQLE DASHAIPPKK CTELKRSRSV KCLGYVVFGY FAVIHVLGFV LVFLYITHVP TASAPLNKKG IN IVLFSLS VTVASCANAG LVPTNENMVI FSKNSGLLLL LSGQMLAGNT LFPLFLRLLV WFLGKLTKVK ELRLMTKNPE EVH FANLLP RLPTVFLSST VIGIVAAGVT LFCSVDWNSS VFDGLGSYQK TVNAFFMVVN ARHSGENSID CSLMSPAIVV LFIG MMYLP SSATFAPPSG DTKTTNENTK GKGKRGSLVQ NLAFSPLGCN IIFVIVACIT ERRRLRSDPL NFSTLNMIFE VISAY GNVG LSTGYSCSRL HQLHPEIICQ DMPYSFSGWW SDGGKFLLVL VMLYGRLKVF AVSTGKSWKV UniProtKB: Uncharacterized protein |
-Macromolecule #2: SODIUM ION
Macromolecule | Name: SODIUM ION / type: ligand / ID: 2 / Number of copies: 2 |
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Molecular weight | Theoretical: 22.99 Da |
-Macromolecule #3: water
Macromolecule | Name: water / type: ligand / ID: 3 / Number of copies: 4 / Formula: HOH |
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Molecular weight | Theoretical: 18.015 Da |
Chemical component information | ChemComp-HOH: |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 8 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 1.6 µm / Nominal defocus min: 0.8 µm |
Image recording | Film or detector model: GATAN K2 QUANTUM (4k x 4k) / Average electron dose: 52.0 e/Å2 |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Startup model | Type of model: OTHER |
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Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 2.6 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 47658 |