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Yorodumi- EMDB-34396: Structure of a TRP channel-G protein complex, Class2, additional map 1 -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-34396 | |||||||||
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Title | Structure of a TRP channel-G protein complex, Class2, additional map 1 | |||||||||
Map data | ||||||||||
Sample |
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Keywords | TRP / transient receptor potential / METAL TRANSPORT | |||||||||
Biological species | Homo sapiens (human) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.79 Å | |||||||||
Authors | Won J / Jeong H / Lee HH | |||||||||
Funding support | Korea, Republic Of, 1 items
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Citation | Journal: To Be Published Title: Structure of a TRP channel-G protein complex, class2 Authors: Won J / Jeong H / Lee HH | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_34396.map.gz | 117.9 MB | EMDB map data format | |
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Header (meta data) | emd-34396-v30.xml emd-34396.xml | 11.1 KB 11.1 KB | Display Display | EMDB header |
Images | emd_34396.png | 96.7 KB | ||
Filedesc metadata | emd-34396.cif.gz | 3.6 KB | ||
Others | emd_34396_half_map_1.map.gz emd_34396_half_map_2.map.gz | 115.7 MB 115.7 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-34396 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-34396 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_34396.map.gz / Format: CCP4 / Size: 125 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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Voxel size | X=Y=Z: 1.088 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #1
File | emd_34396_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_34396_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
-Entire : Transient receptor potential
Entire | Name: Transient receptor potentialTransient receptor potential channel |
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Components |
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-Supramolecule #1: Transient receptor potential
Supramolecule | Name: Transient receptor potential / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: Homo sapiens (human) |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 8 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | TFS GLACIOS |
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Electron beam | Acceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.0 µm |
Image recording | Film or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 40.0 e/Å2 |
-Image processing
Startup model | Type of model: PDB ENTRY PDB model - PDB ID: |
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Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.79 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 5344 |